A Reservoir of Pluripotent Phloem Cells Safeguards the Linear Developmental Trajectory of Protophloem Sieve Elements

Gujas, Bojan; Kastanaki, Elizabeth; Sturchler, Alessandra; Cruz, Tânia; Ruiz-Sola, M. Águila; Dreos, René; Eicke, Simona; Truernit, Elisabeth; Rodríguez-Villalón, Antía

doi:10.1016/j.cub.2019.12.043

Cited by 44 publications

(60 citation statements)

References 54 publications

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“…As mentioned before, CLV2 and CRN are required for CLE peptide perception, which is probably due to that CLV2 and CRN interact with BAM3 and enhance the expression of BAM3 in the developing protophloem (Hazak et al 2017). RPK2 is specifically expressed in the phloem pole pericycle and companion cells, but not in the protophloem to perceive CLE45 peptide, which restricts protophloem sieve element (PSE) identity to the PSE position (Gujas et al 2020) (Fig. 3A).…”

Section: Rlk-mediated Signaling Promotes the Differentiation Of The Pmentioning

confidence: 88%

“…CLE signaling in controlling protophloem differentiation and proximal root meristem maintenance is complex. Many receptors, including BAM3, CLV2/CRN, RPK2, and CIK2/CLERK, are involved in the perception of root-active CLE peptides (Depuydt et al 2013;Hazak et al 2017;Ren et al 2019;Gujas et al 2020) (Fig. 3A).…”

Section: Rlk-mediated Signaling Promotes the Differentiation Of The Pmentioning

confidence: 99%

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Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

Zhu

Gou

2020

aBIOTECH

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Generation of the root greatly benefits higher plants living on land. Continuous root growth and development are achieved by the root apical meristem, which acts as a reservoir of stem cells. The stem cells, on the one hand, constantly renew themselves through cell division. On the other hand, they differentiate into functional cells to form diverse tissues of the root. The balance between the maintenance and consumption of the root apical meristem is governed by cell-to-cell communications. Receptor-like protein kinases (RLKs), a group of signaling molecules localized on the cell surface, have been implicated in sensing multiple endogenous and environmental signals for plant development and stress adaptation. Over the past two decades, various RLKs and their ligands have been revealed to participate in regulating root meristem homeostasis. In this review, we focus on the recent studies about RLK-mediated signaling in regulating the maintenance and consumption of the root apical meristem.

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Section: Rlk-mediated Signaling Promotes the Differentiation Of The Pmentioning

confidence: 88%

Section: Rlk-mediated Signaling Promotes the Differentiation Of The Pmentioning

confidence: 99%

Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

Zhu

Gou

2020

aBIOTECH

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“…Arabidopsis ecotype Columbia-0 was used as a wild-type control in all cases with the exception of the experiments related to pin1-3, pin1-5 and and clv3-7, in which Ler was used as a control, respectively. Seeds of pin1-3, pin1-5, pin1,3,6,4,7,8 and PIN1 CVP2::NLS-3xVENUS, CVL1::NLS-3xVENUS, CVP2::GUS and CVL1::GUS have been previously described (Rodriguez-Villalon et al 2014;Rodriguez-Villalon et al 2015;Gujas et al 2020;Carland and Nelson 2009) . Likewise, rpk2, cvp2 cvl1, amiRPK2 cvp2 cvl1 and ops were reported elsewhere (Gujas et al 2020).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Seeds of pin1-3, pin1-5, pin1,3,6,4,7,8 and PIN1 CVP2::NLS-3xVENUS, CVL1::NLS-3xVENUS, CVP2::GUS and CVL1::GUS have been previously described (Rodriguez-Villalon et al 2014;Rodriguez-Villalon et al 2015;Gujas et al 2020;Carland and Nelson 2009) . Likewise, rpk2, cvp2 cvl1, amiRPK2 cvp2 cvl1 and ops were reported elsewhere (Gujas et al 2020). MP::MP-GFP, SHR::SHR-GFP, DR5::NLS-VENUS, PIN1::PIN1::GFP, AUX1::AUX1-YFP and OPS::OPS-GFP translational fusion reporter lines in distinct genotypes were obtained by crossing these lines with the indicated loss-of-function mutants or have been previously published (Rodriguez-Villalon et al 2015).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…was amplified using the following primers: pBAM3_attB4_F: GGGG ACA ACT TTG TAT AGA AAA GTT GCC CTGCTTCCCTAGTTTATCTAATAAATCTGATG and pBAM3_attB1r_R: GGGG AC TGC TTT TTT GTA CAA ACT TGG TGTAACATCAGAAAAATAAAAACAAAAATTTGTCC and fused to NLS-3xVENUS construct as previously described (Gujas et al, 2020). Transgenic plants were generated using floral-dip transformation techniques as previously described (Gujas et al 2017).…”

Section: Cloning and Plant Transformationmentioning

confidence: 99%

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A molecular framework for proximal secondary vein branching in the Arabidopsis thaliana embryo

Kastanaki

Blanco‐Touriñán

Sarazin

et al. 2021

Preprint

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The establishment of a closed vascular network in foliar organs is achieved through the coordinated specification of newly recruited procambial cells, their proliferation and elongation. An important, yet poorly understood component of this process, is secondary vein branching; a mechanism employed in Arabidopsis thaliana cotyledons to extend vascular tissues throughout the organ surface by secondary vein formation. To investigate the underlying molecular mechanism in vein branching, we analyzed at a single-cell level the discontinuous vein network of cotyledon vascular pattern 2 (cvp2) cvp2-like 1 (cvl1). Utilizing live-cell imaging and genetic approaches we uncovered two distinct branching mechanisms during embryogenesis. Similar to wild type, distal veins in cvp2 cvl1 embryos emerged from the bifurcation of cell files contained in the midvein. However, the branching events giving rise to proximal veins are absent in this mutant. Restoration of proximal branching in cvp2 cvl1 cotyledons could be achieved by increasing OCTOPUS dosage as well as by silencing of RECEPTOR LIKE PROTEIN KINASE 2 (RPK2) expression. The RPK2-mediated restriction of proximal branching is auxin and CLE-independent. Our work defines a genetic network conferring plasticity to Arabidopsis embryos to adapt the spatial configuration of vascular tissues to organ growth.

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The brassinosteroid‐responsive protein OCTOPUS is a novel regulator of Arabidopsis thaliana immune signaling

Greenwood,

King,

Melena

et al. 2023

Plant Direct

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Phloem is a critical tissue for transport of photosynthates and extracellular signals in vascular plants. However, it also represents an ideal environment for pathogens seeking access to valuable host nutrients. Although many vascular pathogens induce economically relevant crop damage, there is still little known about the mechanisms by which immune signaling operates through the phloem. An existing phosphoproteomic dataset was mined to identify proteins that were both phosphorylated in response to the defense‐elicitor flagellin (flg22) and expressed in vascular cells. A single candidate, OCTOPUS (OPS), is polarly associated with the plasma membrane of sieve element cells and has been characterized as an inhibitor of brassinosteroid insensitive‐2 in promotion of brassinosteroid‐related phytohormone signaling. The observation that OPS is differentially phosphorylated in response to flg22 led us to the examine whether OPS may also regulate flg22‐induced immune signaling. Two independent alleles of ops exhibited enhanced immunity outputs across multiple signaling branches of PAMP‐triggered immunity (PTI), constitutively and in response to flg22 treatment. Together with our observation that interactions between OPS and brassinosteroid insensitive‐2 were disrupted by induction of salicylic acid and depletion of brassinosteriod, these data support a model whereby OPS modulates brassinolide and immune signaling to control downstream responses. We present OPS as a novel addition to the list of proteins with documented roles in PAMP‐PTI signaling. These results further indicate that immune signaling in the phloem may be a significant and unique component of the host detection and response to pathogens in vascular plants.

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A Reservoir of Pluripotent Phloem Cells Safeguards the Linear Developmental Trajectory of Protophloem Sieve Elements

Cited by 44 publications

References 54 publications

Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

A molecular framework for proximal secondary vein branching in the Arabidopsis thaliana embryo

The brassinosteroid‐responsive protein OCTOPUS is a novel regulator of Arabidopsis thaliana immune signaling

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