A resettable dynamic microarray device

Iwai, Kosuke; Tan, Wei-Heong; Ishihara, Hirotaka; Takeuchi, Shoji

doi:10.1007/s10544-011-9578-7

Cited by 31 publications

(23 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our designs used hydrodynamic traps to hold islets as they have previously been used to shelter both single cells and larger objects (e.g., bubbles) [15][16][17][18][19][20][21][22][23] …”

Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Highly efficient adenoviral transduction of pancreatic islets using a microfluidic device

et al. 2016

View full text Add to dashboard Cite

Tissues are challenging to genetically manipulate due to limited penetration of viral particles resulting in low transduction efficiency. We are particularly interested in expressing genetically-encoded sensors in ex vivo pancreatic islets to measure glucose-stimulated metabolism, however poor viral penetration biases these measurements to only a subset of cells at the periphery. To increase mass transfer of viral particles, we designed a microfluidic device that holds islets in parallel hydrodynamic traps connected by an expanding by-pass channel. We modeled viral particle flow into the tissue using fluorescently-labelled gold nanoparticles of varying sizes and showed a penetration threshold of only ∼5 nm. To increase this threshold, we used EDTA to transiently reduce cell-cell adhesion and expand intercellular space. Ultimately, a combination of media flow and ETDA treatment significantly increased adenoviral transduction to the core of the islet. As proof-of-principle, we used this protocol to transduce an ER-targeted redox sensitive sensor (eroGFP), and revealed significantly greater ER redox capacity at core islet cells. Overall, these data demonstrate a robust method to enhance transduction efficiency of islets, and potentially other tissues, by using a combination of microfluidic flow and transient tissue expansion.

show abstract

“…Our designs used hydrodynamic traps to hold islets as they have previously been used to shelter both single cells and larger objects (e.g., bubbles) [15][16][17][18][19][20][21][22][23] …”

Section: Microfluidic Device Fabricationmentioning

confidence: 99%

Highly efficient adenoviral transduction of pancreatic islets using a microfluidic device

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The herringbone structure, designed and further improved herein, was tested in conjunction with an array of trapping structures with a goal to enhance trapping efficiency [8,9]. The following equation represents trapping efficiency Eq.…”

Section: Application Of the Device To Cell Trappingmentioning

confidence: 99%

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Jung

Hyun

Choi

et al. 2017

Micro and Nano Syst Lett

View full text Add to dashboard Cite

Controlling cells' movement is an important technique in biological analysis that is performed within a microfluidic system. Many external forces are utilized for manipulation of cells, including their position in the channel. These forces can effectively control cells in a desired manner. Most of techniques used to manipulate cells require sophisticated set-ups and equipment to generate desired effect. The exception to this is the use of hydrodynamic force. In this study, a series of continuously varying herringbone structures is proposed for positioning cells in a microfluidic channel using hydrodynamic force. This structure was experimentally developed by changing parameters, such as the length of the herringbone's apex, the length of the herringbone's base and the ratio of the height of the flat channel to the height of the herringbone structure. Results of this study, have demonstrated that the length of the herringbone's apex and the ratio of the heights of the flat channel and the herringbone structure were crucial parameters influencing positioning of cells at 100 μl/h flow rate. The final design was fixed at 170 and 80 μm for the length of herringbone's apex and the length of herringbone's base, respectively. The average position of cells in this device was 34 μm away from the side wall in a 200 μm wide channel. Finally, to substantiate a practical application of the herringbone structure for positioning, cells were randomly introduced into a microfluidic device, containing an array of trapping structures together with a series of herringbone structures along the channel. The cells were moved toward the trapping structure by the herringbone structure and the trapping efficiency was increased. Therefore, it is anticipated that this device will be utilized to continuously control cells' position without application of external forces.

show abstract

“…The red (solid) and blue (empty) arrows indicate the flow direction during the processes for loading a bead (i.e., trapping, releasing, and loading modes) and dispensing a bead-encapsulating droplet (i.e., dispensing mode), respectively. In the trapping mode, beads migrate laterally against the flow direction along the micropillars to follow the trapping stream, which enters the loading channel, leading to an effective inflow into the bead trap from the main channel23. On the basis of the dynamic change in hydraulic resistance, the valve containing a bead prevents the trapping of additional beads20.…”

Section: Methodsmentioning

confidence: 99%

Deterministic bead-in-droplet ejection utilizing an integrated plug-in bead dispenser for single bead–based applications

Kim

Choi

Lee

et al. 2017

Sci Rep

View full text Add to dashboard Cite

This paper presents a deterministic bead-in-droplet ejection (BIDE) technique that regulates the precise distribution of microbeads in an ejected droplet. The deterministic BIDE was realized through the effective integration of a microfluidic single-particle handling technique with a liquid dispensing system. The integrated bead dispenser facilitates the transfer of the desired number of beads into a dispensing volume and the on-demand ejection of bead-encapsulated droplets. Single bead–encapsulated droplets were ejected every 3 s without any failure. Multiple-bead dispensing with deterministic control of the number of beads was demonstrated to emphasize the originality and quality of the proposed dispensing technique. The dispenser was mounted using a plug-socket type connection, and the dispensing process was completely automated using a programmed sequence without any microscopic observation. To demonstrate a potential application of the technique, bead-based streptavidin–biotin binding assay in an evaporating droplet was conducted using ultralow numbers of beads. The results evidenced the number of beads in the droplet crucially influences the reliability of the assay. Therefore, the proposed deterministic bead-in-droplet technology can be utilized to deliver desired beads onto a reaction site, particularly to reliably and efficiently enrich and detect target biomolecules.

show abstract

A resettable dynamic microarray device

Cited by 31 publications

References 23 publications

Highly efficient adenoviral transduction of pancreatic islets using a microfluidic device

Highly efficient adenoviral transduction of pancreatic islets using a microfluidic device

Manipulation of cells’ position across a microfluidic channel using a series of continuously varying herringbone structures

Deterministic bead-in-droplet ejection utilizing an integrated plug-in bead dispenser for single bead–based applications

Contact Info

Product

Resources

About