A resource for cell line authentication, annotation and quality control

Yu, Mamie; Selvaraj, S.; Liang-Chu, May M.Y.; Aghajani, Sahar; Busse, Matthew; Yuan, Jean; Lee, Genee; Peale, Franklin; Klijn, Christiaan; Bourgon, Richard; Kaminker, Joshua S.; Neve, Richard M.

doi:10.1038/nature14397

Cited by 196 publications

(195 citation statements)

References 30 publications

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“…It has been known for years that many antibodies and small molecule reagents do not-or do not only-detect what it says on the box, yet antibody validation efforts such as antibodypedia are still not routine (Björling & Uhlén, 2008;Baker, 2015). We have known since the 1960s that many cell lines in common use are contaminated or mislabeled (Yu et al, 2015), yet authentication is not a standard publishing requirement. A precondition for reproducibility is to capture as many experimental variables as precisely as possible.…”

Section: Shaken Not Stirredmentioning

confidence: 99%

Reproducibility blues

Pulverer

2015

The EMBO Journal

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Section: Shaken Not Stirredmentioning

confidence: 99%

Reproducibility blues

Pulverer

2015

The EMBO Journal

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“…S3 2 ) (Yu et al, 2015). Mycoplasma was tested after the cells were received and periodically by several of the laboratories using the ATCC assay and all tests were negative.…”

Section: Cell Linesmentioning

confidence: 99%

Toward achieving harmonization in a nanocytotoxicity assay measurement through an interlaboratory comparison study

Elliott

Rösslein

Song

et al. 2017

ALTEX

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SummaryDevelopment of reliable cell-based nanotoxicology assays is important for evaluation of potentially hazardous engineered nanomaterials. Challenges to producing a reliable assay protocol include working with nanoparticle dispersions and living cell lines, and the potential for nano-related interference effects. Here we demonstrate the use of a 96-well plate design with several measurement controls and an interlaboratory comparison study involving five laboratories to characterize the robustness of a nanocytotoxicity MTS cell viability assay based on the A549 cell line. The consensus EC 50 values were 22.1 mg/L (95% confidence intervals 16.9 mg/L to 27.2 mg/L) and 52.6 mg/L (44.1 mg/L to 62.6 mg/L) for positively charged polystyrene nanoparticles for the serum-free and serum conditions, respectively, and 49.7 µmol/L (47.5 µmol/L to 51.5 µmol/L) and 77.0 µmol/L (54.3 µmol/L to 99.4 µmol/L) for positive chemical control cadmium sulfate for the serum-free and serum conditions, respectively. Results from the measurement controls can be used to evaluate the sources of variability and their relative magnitudes within and between laboratories. This information revealed steps of the protocol that may need to be modified to improve the overall robustness and precision. The results suggest that protocol details such as cell line ID, media exchange, cell handling, and nanoparticle dispersion are critical to ensure protocol robustness and comparability of nanocytotoxicity assay results. The combination of system control measurements and interlaboratory comparison data yielded insights that would not have been available by either approach by itself.

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“…Single nucleotide polymorphisms (SNP) analysis is a less-widely adopted but equally accurate method to DNA profile human biosamples (8,11). There are a number of advantages and disadvantages to using each method of which users should be aware (12).…”

Section: Cell Line Authenticationmentioning

confidence: 99%

“…These resources provide an excellent centralized knowledge base for cell line information however, redundancies are still present. To help to address this, a database of highly curated information including cell line annotation using a controlled vocabulary was recently developed for more than 3,000 cell lines (8).…”

Section: Cell Line Nomenclaturementioning

confidence: 99%