A restorer‐of‐fertility‐like pentatricopeptide repeat protein promotes cytoplasmic male sterility in <i>Arabidopsis thaliana</i>

Durand, Simon; Ricou, Anthony; Simon, Matthieu; Dehaene, Noémie; Budar, Françoise; Camilleri, Christine

doi:10.1111/tpj.15045

Cited by 13 publications

(20 citation statements)

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“…The location of the Rf loci found in previous cases of CMS involving the Sha accession partially overlap with those reported in our study (Simon et al, 2016;Durand et al, 2020). The degree of fertility described for CMS found in the Sha x Mr-0 interaction, resulted from a complex interplay between nuclear segregation distorter (pollen killer) loci and several Rf loci (Simon et al, 2016).…”

Section: Identification Of Rf Locisupporting

confidence: 81%

“…The degree of fertility described for CMS found in the Sha x Mr-0 interaction, resulted from a complex interplay between nuclear segregation distorter (pollen killer) loci and several Rf loci (Simon et al, 2016). The three strongest of these Rf loci (named L1, L3 and L5) were also found in the analysis of the Sha x Cvi-0 CMS (Durand et al, 2020).…”

Section: Identification Of Rf Locimentioning

confidence: 94%

“…The number of involved Rf loci underlying CMS-Rf interactions varies between different types of CMS (Li et al, 1998;Wang et al, 2006;Kumar et al, 2012;Durand et al, 2020). These interactions often involve regulation of mitochondrial encoded CMS transcripts via pentatricopeptide repeat (PPR) proteins (Gaborieau et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Exploring the unexplored : Unravelling the cyto-nuclear interactions in Arabidopsis thaliana to improve photosynthesis

Theeuwen

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In the previously described case of the PsbA-mediated resistance to atrazine in A. thaliana, it was found that the selection on this PsbA mutation was sufficiently strong, to ensure that the entire nuclear genome could hitchhike along with the PsbA mutation across the British Railroads (Flood et al., 2016a). This demonstrates how variation in the organellar genomes, can impose a selective pressure on nuclear genomes. Over time, such selection pressures can result in phenotypic variation that is dependent on specific alleles at the organelle side as well as on the nuclear side. A phenotypic effect that is only visible when specific alleles on both the organellar and nuclear genome are combined, is the result of variation within cyto-nuclear interactions. Analysis of mapping populations based on reciprocal hybrids revealed that indeed the phenotypic differences observed are not solely conferred by organellar genetic variation, but also from interactions with nuclear genetic variation (

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Section: Identification Of Rf Locisupporting

confidence: 81%

Section: Identification Of Rf Locimentioning

confidence: 94%

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Exploring the unexplored : Unravelling the cyto-nuclear interactions in Arabidopsis thaliana to improve photosynthesis

Theeuwen

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“…Observations were made using a Zeiss Axio Imager2 microscope and photographs were taken using an AxioCam MRm (Zeiss) camera. Propidium iodide and Alexander staining (Alexander 1969) of pollen were performed as described in Durand et al (2021).…”

Section: Cytological Analysesmentioning

confidence: 99%

APOK3, a pollen killer antidote in Arabidopsis thaliana

et al. 2022

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The principles of heredity state that the two alleles carried by a heterozygote are equally transmitted to the progeny. However, genomic regions that escape this rule have been reported in many organisms. It is notably the case of genetic loci referred to as gamete killers, where one allele enhances its transmission by causing the death of the gametes that do not carry it. Gamete killers are of great interest, particularly to understand mechanisms of evolution and speciation. Although being common in plants, only a few, all in rice, have so far been deciphered to the causal genes. Here, we studied a pollen killer found in hybrids between two accessions of Arabidopsis thaliana. Exploring natural variation, we observed this pollen killer in many crosses within the species. Genetic analyses revealed that three genetically linked elements are necessary for pollen killer activity. Using mutants, we showed that this pollen killer works according to a poison-antidote model, where the poison kills pollen grains not producing the antidote. We identified the gene encoding the antidote, a chimeric protein addressed to mitochondria. De novo genomic sequencing in twelve natural variants with different behaviors regarding the pollen killer revealed a hyper variable locus, with important structural variations particularly in killer genotypes, where the antidote gene recently underwent duplications. Our results strongly suggest that the gene has newly evolved within A. thaliana. Finally, we identified in the protein sequence polymorphisms related to its antidote activity.

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“…For example, MutS HOMOLOG1 (MSH1) affects fertility reversion from cytoplasmic male sterility in Brassica juncea ( Zhao et al, 2021b ). The RFL24 protein in Arabidopsis thaliana is a mitochondrial-targeted pentatricopeptide repeat that can inhibit mitochondrial genes related to male gametes to cause cytoplasmic male sterility ( Durand et al, 2021 ). A recent study found that Rf1 and Rf3 candidates in hybrid wheat can bind to the mitochondrial orf279 transcript and induce its cleavage to prevent the expression of cytoplasmic male sterility, thereby restoring normal pollen production in transgenic wheat plants ( Geyer et al, 2016 ; Melonek et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.

Pei

Xie

Wang

et al. 2022

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Background Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1,645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions We screened 1,645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.

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A restorer‐of‐fertility‐like pentatricopeptide repeat protein promotes cytoplasmic male sterility in Arabidopsis thaliana

Cited by 13 publications

References 63 publications

Exploring the unexplored : Unravelling the cyto-nuclear interactions in Arabidopsis thaliana to improve photosynthesis

Exploring the unexplored : Unravelling the cyto-nuclear interactions in Arabidopsis thaliana to improve photosynthesis

APOK3, a pollen killer antidote in Arabidopsis thaliana

Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.

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