A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

Cuervo‐Arango, J.; Claes, A.; Stout, T.A.E.

doi:10.1016/j.theriogenology.2019.04.010

Cited by 40 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall OPU-ICSI results in this study are comparable to those reported elsewhere [1,45] with 61.2% of the mares producing at least one embryo and with an average of 1.4 (±0.2) blastocysts per session. Cuervo-Arango et al [45] observed that a significantly greater number of blastocysts was obtained per session of OPU-ICSI (1.1 ± 1.4) than with embryo flush (0.64 ± 0.6). These competitive results lead to an increased popularity of OPU-ICSI for breeding valuable horses.…”

Section: Discussionsupporting

confidence: 86%

“…Moderate to strong correlations of AMH concentrations were found within and across breeding seasons for individual mares [41], and the in vitro embryo production capacity of donor mares was shown to be repeatable across OPU-ICSI procedures [45]. In addition, one recent abstract reported the usefulness of determination of AMH concentration as a predictor of oocyte recovery and in vitro production of equine embryo in warmblood mares [60].…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Papas

Govaere

Peere

et al. 2021

Animals

View full text Add to dashboard Cite

Anti-Müllerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In the horse, clinical application of ovum pick-up and intracytoplasmic sperm injection (OPU-ICSI) is increasing, but results depend largely on the individuality of the mare. The aim of this study was to assess AMH as a predictor for the OPU-ICSI outcome in horses. Therefore, 103 mares with a total follicle count above 10 were included in a commercial OPU-ICSI session and serum AMH was determined using ELISA. Overall, the AMH level was significantly correlated with the number of aspirated follicles and the number of recovered oocytes (p < 0.001). Mares with a high AMH level (≥2.5 µg/L) yielded significantly greater numbers of follicles (22.9 ± 1.2), oocytes (13.5 ± 0.8), and blastocysts (2.1 ± 0.4) per OPU-ICSI session compared to mares with medium (1.5–2.5 µg/L) or low AMH levels (<1.5 µg/L), but no significant differences in blastocyst rates were observed. Yet, AMH levels were variable and 58% of the mares with low AMH also produced an embryo. In conclusion, measurement of serum AMH can be used to identify mares with higher chances of producing multiple in vitro embryos, but not as an independent predictor of successful OPU-ICSI in horses.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Papas

Govaere

Peere

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…Scoggin [ 7 ] reported a significant lower embryo mortality rate in mares aged 2–8 than in mares older than 14 years. However [ 8 ], did not find significant differences in embryo mortality rate among age classes. According to age, a lower embryonic mortality in YN (3.2%) than OD (50%) (P < 0.01).…”

Section: Discussionmentioning

confidence: 99%

“…In horse husbandry, the role of advancing age in mares on decreased fertility and pregnancy rate has been investigated [ 7 , 8 , 9 , 10 , 11 ], even though different cut-off ages have been suggested [ 10 ], as well as different criteria for grouping mares in age classes [ 6 , 8 , 9 ]. Foaling rate was also reported to be influenced by mares' age, with predictable lower live-foal rate in mares aged >18 years old than younger mares [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of age and of reproductive status on reproductive indices in horse mares carrying mule pregnancies

Carluccio

Bucci

Fusi

et al. 2020

Heliyon

View full text Add to dashboard Cite

Mules (Equus mulus) are interspecific hybrids derived from crossing horse (Equus caballus) mares with jackasses (Equus asinus). In Italy, the production of mules is very important for the special environmental areas, where they are used as working animals, instead of the forbidden agricultural machines. Although many horse mares carry mule pregnancy successfully at term, low fertility of mares used for producing mules was reported, but limited data about reproductive efficiency in mares carrying mule pregnancy, especially when age and reproductive status are concerned, are available. Therefore, the present study aimed to assess the effect of age and of reproductive status on some reproductive indices of horse mares carrying mule pregnancy, in the final purpose to better clarify factors affecting reproductive performances in the valuable production of mules as working animals under special environmental areas in Italy. The study, performed on 96 draft mares inseminated with Martina Franca donkey semen, showed a satisfactory 89.6% 14 days PO pregnancy rate, lower pregnancy losses (19.8%) than those previously reported for mule pregnancies, leading to satisfactory foaling rates (71.9%), similar to those reported for intraspecific horse pregnancies. A clear, significant detrimental effect of mare's age on pregnancy losses, foaling rate, and on number of estrous cycles needed to achieve pregnancy, was found. Reproductive status also affected the reproductive indices, with significantly higher pregnancy losses and lower foaling rate in barren mares, reopening the question about the possible lower reproduction efficiency in horse mares carrying mule pregnancy. The present study results, although mostly descriptive, add useful information about some factors affecting reproductive performances in the valuable production of mules as working animals under special environmental areas in Italy.

show abstract

“…Thus, the choice of ART in both species is related to maternal aging. In the equine industry, OPU–ICSI–ET is essentially used in older mares, with embryo transfer in younger, healthy recipient mares so as to reduce age-related potential effects [ 198 ].…”

Section: Effects Of Maternal Environment In Equine and Humanmentioning

confidence: 99%

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Benammar

Derisoud

Vialard

et al. 2021

Animals

View full text Add to dashboard Cite

Although there are large differences between horses and humans for reproductive anatomy, follicular dynamics, mono-ovulation, and embryo development kinetics until the blastocyst stage are similar. In contrast to humans, however, horses are seasonal animals and do not have a menstrual cycle. Moreover, horse implantation takes place 30 days later than in humans. In terms of artificial reproduction techniques (ART), oocytes are generally matured in vitro in horses because ovarian stimulation remains inefficient. This allows the collection of oocytes without hormonal treatments. In humans, in vivo matured oocytes are collected after ovarian stimulation. Subsequently, only intra-cytoplasmic sperm injection (ICSI) is performed in horses to produce embryos, whereas both in vitro fertilization and ICSI are applied in humans. Embryos are transferred only as blastocysts in horses. In contrast, four cells to blastocyst stage embryos are transferred in humans. Embryo and oocyte cryopreservation has been mastered in humans, but not completely in horses. Finally, both species share infertility concerns due to ageing and obesity. Thus, reciprocal knowledge could be gained through the comparative study of ART and infertility treatments both in woman and mare, even though the horse could not be used as a single model for human ART.

show abstract

A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

Cited by 40 publications

References 42 publications

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare

Effect of age and of reproductive status on reproductive indices in horse mares carrying mule pregnancies

The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?

Contact Info

Product

Resources

About