2000
DOI: 10.1073/pnas.170283697
|View full text |Cite
|
Sign up to set email alerts
|

A retroviral RNA kissing complex containing only two G⋅C base pairs

Abstract: The dimerization of viral RNA through noncovalent interactions at their 5 ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using twodimensional NMR, we determined the structure of the H3 stemloop. Surprisingly, it forms a stable, homodimeric kissing complex thro… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

5
117
0

Year Published

2008
2008
2021
2021

Publication Types

Select...
4
4

Relationship

0
8

Authors

Journals

citations
Cited by 107 publications
(122 citation statements)
references
References 31 publications
5
117
0
Order By: Relevance
“…SHAPE uses a chemical reaction at the RNA 2′-hydroxyl position to measure local nucleotide flexibility (34,35); flexibility can be reduced either by base pairing or by bound protein. We found that the two RNA strands in the dimer are held together by intermolecular base pairs in two palindromic stretches, termed PAL1 and PAL2, and by G-C base-pairing interactions in a highly conserved double stem-loop motif (SL1-SL2) (31,33,(36)(37)(38). These elements are separated by two distinct flexible elements, between PAL1 and SL0 and between PAL2 and SL1, respectively (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…SHAPE uses a chemical reaction at the RNA 2′-hydroxyl position to measure local nucleotide flexibility (34,35); flexibility can be reduced either by base pairing or by bound protein. We found that the two RNA strands in the dimer are held together by intermolecular base pairs in two palindromic stretches, termed PAL1 and PAL2, and by G-C base-pairing interactions in a highly conserved double stem-loop motif (SL1-SL2) (31,33,(36)(37)(38). These elements are separated by two distinct flexible elements, between PAL1 and SL0 and between PAL2 and SL1, respectively (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…NMR data recorded on weakly aligned samples allowed us to obtain a structural model in solution of an RNA-RNA kissing complex with higher accuracy and precision than previously reported kissing complex solution structures (14,20,(22)(23)(24)(25)(26). This structural study revealed the key atomic interactions responsible for the stabilizing role of the GA base pair at the loop-closing position of kissing complexes.…”
mentioning
confidence: 88%
“…First, the number of paired loop residues can vary from two up to seven (22,23,35). In addition, some of the kissing complexes possess nonpaired bases (22,24,25,(47)(48)(49).…”
Section: Curvature Of Kissing Complexes Lacking Unpaired Residuesmentioning
confidence: 99%
See 1 more Smart Citation
“…Psl-3 and Psl-4 are positioned at the same distance from the primer binding site in RD114, as are two psls DIS-1 and DIS-2 of the MLVs. Moreover, two GACGhairpins downstream from DIS-2 are implicated in the dimerization and packaging of MLV-RNA (Mougel and Barklis 1997;De Tapia et al 1998;Fisher and Goff 1998;Kim and Tinoco 2000;Badorrek et al 2006). Such GACGhairpin motifs are also found in RD114 RNA (as well as in BaEV and PcEV), with GACG-sequences at nucleotide positions 336-339 and 371-375, respectively (see Fig.…”
Section: Discussionmentioning
confidence: 99%