A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria

Umeda, Sumiyo; Stagliano, Gregory W.; Borenstein, Michael; Raffa, Robert B.

doi:10.1016/j.vascn.2004.07.002

Cited by 39 publications

(23 citation statements)

References 13 publications

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“…To find a counterexample to "if linear, then r = 1" it suffices to show that there is something that has r = 1 but it is not linear. Appendix A provides a comprehensive counterexample based on statistical analysis of the various error sum squares and variance components from a linear calibration data set reported in the literature [45]. Before studying Appendix A, some important aspects such as replication, error sum squares and degrees of freedom should be discussed in advance.…”

Section: Linear Calibration Functionmentioning

confidence: 99%

Key aspects of analytical method validation and linearity evaluation

Araujo¹

2009

Journal of Chromatography B

389

223

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Section: Linear Calibration Functionmentioning

confidence: 99%

Key aspects of analytical method validation and linearity evaluation

Araujo¹

2009

Journal of Chromatography B

389

223

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“…A variety of methods have been developed to measure serotonin including spectrophotometry [21,22], LCfluorometry [23,24], enzyme immunoassay, and LCelectrochemical detection [25,26]. LC-mass spectrometric methods is however the method of choice as tandem LC-MS methods provide increased selectivity while reducing the need for dependence on chromatographic separation [27][28][29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

et al. 2011

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Serotonin is a major neurotransmitter and affects various functions both in the brain and in the rest of the body. It has been demonstrated that altered serotinergic function is implicated in various psychiatric disorders including depression and schizophrenia. Serotonin has also been implicated along with dopamine in attention deficithyperkinetic disorder (AD-HKD). This study provides a versatile validated method for the analysis of serotonin, hydroxyindole acetic acid and dopamine in urine using LC-MS/MS. This method was then used to quantify these analytes in a test group of 17 children diagnosed with severe AD-HKD. This group was compared to a matched control group to investigate the possibility that one of these compounds may be a potential biomarker for this condition. The developed method provided good linear calibration curves for the multiplex assay of analytes in urine (0.05-3.27 nmol/L; R 2 ≥0.9977). Acceptable inter-day repeatability was achieved for all analytes with RSD values (n=9) ranging from 1.1% to 9.3% over a concentration range of 0.11-3.27 μmol/L in urine. Excellent limits of detection (LOD) and limits of quantitation (LOQ) were achieved with LODs of 8.8-18.2 nmol/L and the LOQs of 29.4-55.7 nmol/L for analytes in urine. Recoveries were in the ranges of 98-104%, 100-106% and 91-107% for serotonin, 5-HIAA and dopamine, respectively. An appropriate sample clean-up procedure for urine was developed to ensure efficient recovery and reproducibility on analysis. Evaluation of matrix effects was also carried out and the influence of ion suppression on analytical results reported. Confirmatory analysis was carried out on a linear trap quadrupole-Orbitrap mass spectrometer to obtain high mass accuracy data of the target analytes in the clinical samples.

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“…Methamphetamine-induced withdrawal was not affected by a 5-HT 2B/2C agonist (m-CPZ), suggesting that the abstinence response was selective for 5-HT 1A compounds. A major role for 5-HT 1A -like receptors in planarian methamphetamine physical dependence is supported by evidence that planarians display characteristic behavioral responses following acute 5-HT exposure and express 5-HT 1A -like receptors that show partial homology to mammalian 5-HT 1A receptors which contribute to the psychostimulant-induced 5-HT response [3, 32, 34, 36–37, 39]. Four sequences of 5-HT receptors in planarians, designated 5-HTpla1 to 5-HTpla4, have been identified in molecular biology studies, all of which are similar to the human 5-HT 1A receptor.…”

Section: Discussionmentioning

confidence: 99%

“…Rodents develop methamphetamine physical dependence, but the associated withdrawal symptoms are more difficult to quantify than opiate-induced withdrawal phenomena [6]. A simpler species that displays a quantifiable abstinence-induced withdrawal response to psychostimulants is planarians, aquatic flatworms that utilize mammalian-relevant neurotransmitters, such as serotonin (5-HT), dopamine, and glutamate, that mediate addiction [4, 15, 17, 25–31, 39]. Methamphetamine produces alterations in 5-HT function in mammals which underlie its addictive properties [8, 12, 14, 38].…”

Section: Introductionmentioning

confidence: 99%

5-HT1A-like receptor activation inhibits abstinence-induced methamphetamine withdrawal in planarians

Shah

Ayoub

Raffa

2010

Neuroscience Letters

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No pharmacological therapy is approved to treat methamphetamine physical dependence, but it has been hypothesized that serotonin (5-HT)-enhancing drugs might limit the severity of withdrawal symptoms. To test this hypothesis, we used a planarian model of physical dependence that quantifies withdrawal as a reduction in planarian movement. Planarians exposed to methamphetamine (10 µM) for 60 min, and then placed (tested) into drug-free water for 5 min, displayed less movement (i.e., withdrawal) than either methamphetamine-naïve planarians tested in water or methamphetamine-exposed planarians tested in methamphetamine. A concentration-related inhibition of withdrawal was observed when methamphetamine-exposed planarians were placed into a solution containing either methamphetamine and 5-HT (0.1 – 100 µM) or methamphetamine and the 5-HT1A receptor agonist 8-Hydroxy-N,N-dipropyl-2-aminotetralin (8-OH-DPAT) (10, 20 µM). Planarians with prior methamphetamine exposure displayed enhanced withdrawal when tested in a solution of the 5-HT1A receptor antagonist N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]- N-(2-pyridyl)cyclohexanecarboxamide (WAY 100635) (1 µM). Methamphetamine-induced withdrawal was not affected by the 5-HT2B/2C receptor agonist meta-chlorophenylpiperazine (m-CPZ) (0.1 – 20 µM). These results provide pharmacological evidence that serotonin-enhancing drugs inhibit expression of methamphetamine physical dependence in an invertebrate model of withdrawal, possibly through a 5-HT1A-like receptor-dependent mechanism.

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A reverse-phase HPLC and fluorescence detection method for measurement of 5-hydroxytryptamine (serotonin) in Planaria

Cited by 39 publications

References 13 publications

Key aspects of analytical method validation and linearity evaluation

Key aspects of analytical method validation and linearity evaluation

Development of an LC-MS/MS method for the analysis of serotonin and related compounds in urine and the identification of a potential biomarker for attention deficit hyperactivity/hyperkinetic disorder

5-HT1A-like receptor activation inhibits abstinence-induced methamphetamine withdrawal in planarians

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