A Reversed-Phase Thin-Layer Chromatography/Scanning Densitometric Method for the Analysis of Gardenia Yellow in Food Using Crocetin as an Indicator

Ozeki, Naoko; Oka, Hisao; Ito, Yûko; Ueno, Eiji; Goto, Tomomi; Hayashi, Tomoko; Itakura, Yûko; Ito, Toru; Maruyama, Takashi; Turuta, Masukiyo; Miyazawa, Takahiko; Matsumoto, Hiroshi

doi:10.1081/jlc-100106953

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…[7] As shown in Table 1, the average Ra/Rs values were 1.00, 1.01, and 1.02 with the coefficients of variation of 3.2%, 4.8%, and 8.1% for spots 1, 2, and 3, respectively. The obtained R f values of the spots were then compared.…”

Section: Reproducibility Of the R F Value By Reverse-phase Tlcmentioning

confidence: 88%

A Reversed‐Phase Thin‐Layer Chromatography/Scanning Densitometric Method for the Qualitative Analysis of Carthamus Yellow in Foods

Watanabe¹,

Ito

Oka

et al. 2005

Journal of Liquid Chromatography & Related Technologies

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A technique for the analysis of carthamus yellow using reversed-phase TLC and scanning densitometry is described. The technique involves the following three steps: (1) clean-up of the color with a C18 and a DEA cartridge; (2) separation of the colors on the reversed-phase C18-TLC using 2-butanone : methanol : 5% sodium sulfate : 5% acetic acid (3 : 2 : 5 : 5) as the solvent system; and (3) measurement of visible absorption spectrum of the color using scanning densitometry without isolation of the color. In order to investigate the capability of the present method, 35 commercial foods were analyzed, and their chromatographic behavior and spectra were observed. The obtained separation and the spectra were not affected by coexisting substances in the foods and the spots always gave the same R f values and spectra as the standards with good reproducibility. The present method is considered to be useful for the rapid analysis of carthamus yellow in foods.

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Section: Reproducibility Of the R F Value By Reverse-phase Tlcmentioning

confidence: 88%

A Reversed‐Phase Thin‐Layer Chromatography/Scanning Densitometric Method for the Qualitative Analysis of Carthamus Yellow in Foods

Watanabe¹,

Ito

Oka

et al. 2005

Journal of Liquid Chromatography & Related Technologies

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“…Hiện nay, trên thế giới, có một số nghiên cứu đã được công bố về phương pháp xác định crocin và crocetin trên một số nền mẫu như: nhuỵ hoa nghệ tây (saffron), quả dành dành (chi tử), mì tôm, nước giải khát…sử dụng các kỹ thuật như: sắc ký bản mỏng TLC [3], điện di mao quản CE [4], quang phổ (UV), sắc ký lỏng hiệu năng cao (HPLC) [5][6][7][8][9][10]... Trong đó, phương pháp sắc ký lỏng (HLPC) là kỹ thuật được nhiều nghiên cứu sử dụng do tính phổ biến, độ nhạy, độ chọn lọc và độ chính xác cao, đồng thời quá trình xử lý mẫu trong các nền mẫu thực phẩm tương đối đơn giản. Do đó, nghiên cứu này lựa chọn phương pháp sắc ký lỏng hiệu năng cao HPLC-PDA để xác định đồng thời crocin và crocetin trong thực phẩm.…”

Section: Hình 1 Công Thức Cấu Tạo Của Crocin (A) Và Crocetin (B)unclassified

Simultaneous determination of crocin and crocetin in food samples by highperformance liquid chromatography (HPLC-PDA)

Do Thi Hong

2023

VJFC

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Crocin and crocetin are two bioactive compounds found in saffron and gardenia. In the food industry, due to their natural yellow or orange color, they are used as food colouring. The study was carried out to optimize the high-performance liquid chromatography combined with a photodiode detector (HPLC-PDA) to simultaneously determine the crocin and crocetin content in food. Analytical samples were ultrasonicated with ACN:H2O (1:1, v/v) solvent for 30 minutes. The sample solution was determined by HPLC – PDA with the following conditions: Sunfire C18 column (250 mm x 4.6 mm; 5 µm) mobile phase consisting of 0.1% formic acid and ACN using gradient program. The method has good specificity, the standard curves of crocin and crocetin have correlation coefficients R2 > 0.999, respectively, repeatability and recovery meet AOAC requirements. The limit of detection (LOD) for  crocin and crocetin were 0.5 mg/kg and 0.25 mg/kg, respectively. The method has been applied to simultaneously determine crocin and crocetin in 15 real samples and results in 13 out of 15 samples detecting crocin content, 11 out of 15 samples detecting crocetin content, and in 2 out out of 15 samples. neither crocin nor crocetin was detected. The gardenia powder group contained the highest content of crocin and crocetin (23.5 g/100g – 24.4 g/100g) and dried lychee samples - sun-dried or dried gardenia contains the second highest concentrations of crocin and crocetin in the food group.

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“…By making use of these properties, it is widely used for the coloring of juice, candy, jelly, chewing gum, fruit wine, etc. [1] In our previous studies, [5][6][7][8] we have already established simple, rapid, and reliable methods for the analysis of various natural colors, including annatto, gardenia yellow, carotene, turmeric oleoresin, lac, cochineal, and paprika using reversed-phase thin-layer chromatography=scanning densitometry. Therefore, in this study, we decided to develop a simple, reliable, and rapid analysis method for the red cabbage color in foods using reversed-phase thin-layer chromatography=scanning densitometry, and we describe the techniques for the separation and identification of this color in foods.…”

Section: Introductionmentioning

confidence: 99%

A Reversed-Phase Thin-Layer Chromatography/Scanning Densitometric Method for the Analysis of Red Cabbage Color in Food

Itakura¹,

Ozeki²,

Oka³

et al. 2002

Journal of Liquid Chromatography & Related Technologies

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A technique for the analysis of red cabbage color using reversed-phase TLC and scanning densitometry is described.The technique involves the following three steps: 1) clean up of the color with a C18 cartridge, 2) separation of the colors on the reversed-phase C18-TLC using acetonitrile-0.2 mol=L trifluoroacetic acid (1 : 2) as the solvent system, and 3) measurement of visible absorption spectra of the color using scanning densitometry without isolation of the color. In order to investigate the capability of the present method, 45 commercial foods were analyzed, and their chromatographic behaviors and spectra were observed. The obtained separation and the spectra were not affected by coexisting substances, including grape skin color, elderberry color, perilla color, and cochineal color in the foods, and the spots always gave the same Rf values and spectra as the standards with good reproducibility. The present method is considered to be useful for the rapid analysis of red cabbage color in foods.

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A Reversed-Phase Thin-Layer Chromatography/Scanning Densitometric Method for the Analysis of Gardenia Yellow in Food Using Crocetin as an Indicator

Cited by 11 publications

References 9 publications

A Reversed‐Phase Thin‐Layer Chromatography/Scanning Densitometric Method for the Qualitative Analysis of Carthamus Yellow in Foods

A Reversed‐Phase Thin‐Layer Chromatography/Scanning Densitometric Method for the Qualitative Analysis of Carthamus Yellow in Foods

Simultaneous determination of crocin and crocetin in food samples by highperformance liquid chromatography (HPLC-PDA)

A Reversed-Phase Thin-Layer Chromatography/Scanning Densitometric Method for the Analysis of Red Cabbage Color in Food

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