A reversible near-infrared pH probes for optical measurements of pH in complete water system and living cells

Yu, Huan; Sun, Mingtai; Kui, Zhang; Zhu, Houjuan; Liu, Zhengjie; Zhang, Yajun; Zhao, Jun; Wu, Lijun; Zhang, Zhongping; Wang, Suhua

doi:10.1016/j.snb.2015.05.029

Cited by 16 publications

(8 citation statements)

References 39 publications

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“…The sensor was more emissive at pH 7.0 than that at 7.8; this property has been used to monitor slight intracellular pH changes in HL-7702 cells. Sensor 11 , which consisted of a Cy5 fluorophore and a nitrogen-involved proton regulator, was reported by Wang’s group . This compound fluoresced around 660 nm, and the emission showed small but noticeable red shifts to 623 nm when the pH value decreased from 7.0 to 5.0.…”

Section: Chemosensors Based On Cyaninesmentioning

confidence: 99%

Recent Development of Chemosensors Based on Cyanine Platforms

et al. 2016

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The cyanine platforms including cyanine, hemicyanine, and squaraine are good candidates for developing chemosensors because of their excellent photophysical properties, outstanding biocompatibility, and low toxicity to living systems. A huge amount of research work involving chemosensors based on the cyanine platforms has emerged in recent years. This review focuses on the development from 2000 to 2015, in which cyanine, hemicyanine, and squaraine sensors will be separately summarized. In each section, a systematization according to the type of detection mechanism is established. The basic principles about the design of the chemosensors and their applications as bioimaging agents are clearly discussed. In addition, we emphasize the advances that have been made in improving the detection performance through incorporation of the chemosensors into nanoparticles.

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Section: Chemosensors Based On Cyaninesmentioning

confidence: 99%

Recent Development of Chemosensors Based on Cyanine Platforms

et al. 2016

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“…In the past few years, there has been extensive research on fluorescent dyes for use in optical chemical sensors. New types of indicator dyes have been reported with absorption and emission maxima in the red or NIR spectal range (Yu et al 2015). This is advantageous in biological applications due to the lower spectral interference of biomolecules in the NIR region.…”

Section: Developments Of Optical Sensorsmentioning

confidence: 99%

Novel probes for pH and dissolved oxygen measurements in cultivations from millilitre to benchtop scale

Demuth

Varonier

Jossen

et al. 2016

Appl Microbiol Biotechnol

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pH value and the concentration of dissolved oxygen (DO) are key parameters to monitor and control cell growth in cultivation studies. Reliable, robust and accurate methods to measure these parameters in cultivation systems in real time guarantee high product yield and quality. This mini-review summarises the current state of the art of pH and DO sensors that are applied to bioprocesses from millilitre to benchtop scale by means of a short introduction on measuring principles and selected applications. Special emphasis is placed on single-use bioreactors, which have been increasingly employed in bioprocess development and production in recent years. Working principles, applications and the particular requirements of sensors in these cultivation systems are given. In such processes, optical sensors for pH and DO are often preferred to electrochemical probes, as they allow semi-invasive measurements and can be miniaturised to micrometre scale or lower. In addition, selected measuring principles of novel sensing technologies for pH and DO are discussed. These include solid-state sensors and miniaturised devices that are not yet commercially available, but show promising characteristics for possible use in bioprocesses in the near future.

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“…Fluorescence imaging is frequently used for real-time pH monitoring in biological systems due to its rapid response time, high sensitivity, non-destructive nature, operational simplicity, and high-speed spatial capabilities [ 4 ]. Recently, near-infrared pH fluorescent probes were developed to take advantage of near-infrared imaging unique features such as minimum photobleaching, deep tissue penetration, suppressed photodamage to cells and tissues, and low biological luminescence background [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Most of these near-infrared fluorescent probes that measure pH levels are based on fluorescence changes in a single near-infrared wavelength [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ,…”

Section: Introductionmentioning

confidence: 99%

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Zhang

Xia

et al. 2018

Molecules

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A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

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A reversible near-infrared pH probes for optical measurements of pH in complete water system and living cells

Cited by 16 publications

References 39 publications

Recent Development of Chemosensors Based on Cyanine Platforms

Recent Development of Chemosensors Based on Cyanine Platforms

Novel probes for pH and dissolved oxygen measurements in cultivations from millilitre to benchtop scale

A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

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