A review: Construction of aptamer screening methods based on improving the screening rate of key steps

Wei, Xunjiao; Ma, Peisheng; Mahmood, Khan Imran; Zhang, Yin; Wang, Zhouping

doi:10.1016/j.talanta.2022.124003

Cited by 19 publications

(4 citation statements)

References 128 publications

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“…The full length of an aptamer sequence usually includes non-essential bases that do not interact with the target molecule. Aptamer flexible structures with long sequences can increase the cost and structural instability . The aptamer was further sheared and optimized to improve its affinity.…”

Section: Resultsmentioning

confidence: 99%

“…where Y is the absorption ratio (A 670 /A 520 ), X is the concentration of each target, and the cost and structural instability. 43 The aptamer was further sheared and optimized to improve its affinity. Since regions with specific secondary structures (e.g., stem-loop and hairpin structures) facilitated direct binding to the target, the aptamer sequence was truncated according to its predicted secondary structure (Figure 2).…”

Section: Sequence Analysis Of Selectedmentioning

confidence: 99%

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Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Wang

Liu

Shen

et al. 2023

J. Agric. Food Chem.

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In this work, broad-spectrum aptamers for organophosphorus pesticides (OPs) were obtained by alternate target systematic evolution of ligands by exponential enrichment screening. The secondary and tertiary structure analyses of the aptamer inferred that the neck-loop structure formed a G-triplex structure with the target. In addition, optimization of the sheared aptamer resulted in a stronger affinity (K d = 86.74 nM), which was increased by 2 orders of magnitude compared to similar aptamers. A novel electrochemical biosensor was prepared by modifying an aptamer labeled with an electroactive substance (methylene blue) on the surface of nanoporous carbon containing Fe–Co (Fe–Co/NPC). When a target bound to the aptamer, a G-triplex structure was formed close to the electrode surface. The aptamer phosphate backbone labeled with methylene blue enhanced the electron-transfer efficiency and resulted in signal changes. The biosensor exhibited an excellent sensitivity (7.32 fM) and a wide detection range (1 × 10–13 to 1 × 10–3 M) for OPs under optimal conditions, enabling simultaneous detection of multiple OPs in vegetables.

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Section: Resultsmentioning

confidence: 99%

Section: Sequence Analysis Of Selectedmentioning

confidence: 99%

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Wang

Liu

Shen

et al. 2023

J. Agric. Food Chem.

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“…Aptamer is a segment of ssDNA that shows high affinity and specificity for a target, which is SELEX based on artificially constructed oligonucleotide libraries [ 23 , 24 , 25 , 26 , 27 ]. As recognition progenitors, biosensors bind to targets, thereby transforming from free conformations to city-specific three-dimensional structures, such as hairpin structures, stem loops, and G-quadruplexes [ 28 , 29 , 30 , 31 ]. Biosensors bind specifically to the target through hydrogen bonds, hydrophobic interactions, van der Waals forces, etc., and generate recognition signals through signal conversion elements [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

Wang,

Bao,

Liu

et al. 2024

Sensors

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Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price and broad spectrum of bacterial inhibition, etc. However, it is difficult to degrade and is retained in animal-derived food products, which are hazardous to human health. In this study, a simple and efficient method was developed for the detection of OFL residues in meat products. OFL coupled with amino magnetic beads by an amination reaction was used as a stationary phase. Aptamer AWO-06, which showed high affinity and specificity for OFL, was screened using the exponential enrichment (SELEX) technique. A fluorescent biosensor was developed by using AWO-06 as a probe and graphene oxide (GO) as a quencher. The OFL detection results could be obtained within 6 min. The linear range was observed in the range of 10–300 nM of the OFL concentration, and the limit of the detection of the sensor was 0.61 nM. Furthermore, the biosensor was stored at room temperature for more than 2 months, and its performance did not change. The developed biosensor in this study is easy to operate and rapid in response, and it is suitable for on-site detection. This study provided a novel method for the detection of OFL residues in meat products.

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“…Nguyen et al introduced materials into aptamer screening, which can rapidly adsorb ssDNA by π–π forces with the help of graphene oxide . In recent years, the insertion of structural sequences into libraries has been studied to improve the success rate of aptamer screening. − Davis and Szostak confirmed that the aptamers obtained after screening are from structured libraries . The structured design of the library can reduce the workload of screening.…”

Section: Introductionmentioning

confidence: 99%

Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor

Wei

Mahmood

et al. 2023

J. Agric. Food Chem.

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Aflatoxin M1 (AFM1), a secondary metabolite of Aspergillus spp., is highly toxic and widely present in food matrices. Therefore, the detection of AFM1 is of great importance for the protection of food safety. In this study, a five-segment sequence was designed as the initial library. Graphene oxide-SELEX (GO-SELEX) was applied to screen AFM1. After seven rounds of repeated screening, affinity and specificity assays showed that aptamer 9 was the best candidate for AFM1. The dissociation constant (K d) of aptamer 9 was 109.10 ± 6.02 nM. To verify the efficiency and sensitivity aptamer for the detection of AFM1, a colorimetric sensor based on the aptamer was constructed. The biosensor showed good linearity in the range of AFM1 concentration of 0.5–500.0 ng/mL with a detection limit of 0.50 ng/mL. This colorimetric method was successfully used for the detection of AFM1 in milk powder samples. Its detection recovery was 92.8–105.2%. This study was conducted to provide a reference for the detection of AFM1 in food.

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A review: Construction of aptamer screening methods based on improving the screening rate of key steps

Cited by 19 publications

References 128 publications

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor

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A review: Construction of aptamer screening methods based on improving the screening rate of key steps

Cited by 19 publications

References 128 publications

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Novel Broad-Spectrum Aptamer Used to Build Fe–Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

Screening of a High-Affinity Aptamer for Aflatoxin M1 and Development of Its Colorimetric Aptasensor

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Screening of a High-Affinity Aptamer for Aflatoxin M₁ and Development of Its Colorimetric Aptasensor