A Review of 3-Dimensional Skin Bioprinting Techniques: Applications, Approaches, and Trends

Ishack, Stephanie; Lipner, Shari R.

doi:10.1097/dss.0000000000002378

Cited by 25 publications

(35 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Apart from the use of stem cells, even from burned and debrided skin 205 , future perspectives in the field of TESSs for wound healing are focused on the development of more similar models of artificial skin where 3D bioprinting 211 , designing stem cell niches 212 or incorporation of immune cells 10 , 179 , 180 will play an important role.…”

Section: Discussionmentioning

confidence: 99%

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

et al. 2021

View full text Add to dashboard Cite

Wound healing is an important function of skin; however, after significant skin injury (burns) or in certain dermatological pathologies (chronic wounds), this important process can be deregulated or lost, resulting in severe complications. To avoid these, studies have focused on developing tissue-engineered skin substitutes (TESSs), which attempt to replace and regenerate the damaged skin. Autologous cultured epithelial substitutes (CESs) constituted of keratinocytes, allogeneic cultured dermal substitutes (CDSs) composed of biomaterials and fibroblasts and autologous composite skin substitutes (CSSs) comprised of biomaterials, keratinocytes and fibroblasts, have been the most studied clinical TESSs, reporting positive results for different pathological conditions. However, researchers’ purpose is to develop TESSs that resemble in a better way the human skin and its wound healing process. For this reason, they have also evaluated at preclinical level the incorporation of other human cell types such as melanocytes, Merkel and Langerhans cells, skin stem cells (SSCs), induced pluripotent stem cells (iPSCs) or mesenchymal stem cells (MSCs). Among these, MSCs have been also reported in clinical studies with hopeful results. Future perspectives in the field of human-TESSs are focused on improving in vivo animal models, incorporating immune cells, designing specific niches inside the biomaterials to increase stem cell potential and developing three-dimensional bioprinting strategies, with the final purpose of increasing patient’s health care. In this review we summarize the use of different human cell populations for preclinical and clinical TESSs under research, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

et al. 2021

View full text Add to dashboard Cite

show abstract

“…[49] Full-thickness complex human skin models generated by a 3D cell-printing process may offer another feasible strategy to reflect the complexity of native human skin. [56,57] This complexity is simulated in the skin model by incorporation of an epidermis and vascularized dermal and hypodermal compartments. [58] Of note, such vascularized skin models represent a further step forward to simulate the in vivo situation.…”

Section: D S K In Model S a S Helpful Tool S In Per Sonalized Med mentioning

confidence: 99%

“…In this context, 3D bioprinting using specific bioink offers the possibility to generate biomimetic skin constructs in a fast and reproducible way because the automated process facilitates the deposition of specific cell types at desired positions. [56][57][58] It is intriguing to speculate that the combination of 3D bioprinting with patient-based bioink (including individual microbiota compositions) will allow for the construction of an in vitro copy of an individual diseased skin state. In the light of these recent developments, it will be exciting to see how future research will unravel personalized models to study individual pathophysiological factors contributing to different skin diseases.…”

Section: D S K In Model S a S Helpful Tool S In Per Sonalized Med mentioning

confidence: 99%

Skin microbiota analysis in human 3D skin models—“Free your mice”

Emmert

Rademacher

Gläser

et al. 2020

Experimental Dermatology

View full text Add to dashboard Cite

There is rapid development in the generation of various human skin models. These encompass simple 2D models and more sophisticated 3D models [1,2] including immunocompetent skin models. [3] Human skin models offer one major advantage that should favour their future use: They are human! This simple statement may have important implications when investigating human skin-microbiota interactions. Many mouse models are in use to analyse skin physiology and biology, and it is beyond question that such mouse models help to address specific in vivo issues. However, mouse skin differs from human skin in many ways. In addition to differences in the anatomical structure (mouse skin is thinner due to fewer keratinocytes layers and contains more hair follicles), there are marked differences in gene expression. Gerber et al discovered that there is only 30% identity between the top human-and mouse skin-associated genes. The authors conclude that this huge diversity may explain why data generated in mouse models often fail to translate into humans. [4] There are also important differences in the composition of barrier-related structure proteins building the epidermal differentiation complex. [5] Moreover, human skin harbours different subsets of immune cells and produces different cytokines as compared to mouse skin. [6] Similarly, there are also distinct differences between skin-derived mouse and human antimicrobial peptides (AMPs), for example RNase 7 or SKALP (skin-derived antileukoprotease). This may have crucial consequences because AMPs are an important component of innate cutaneous defense, and there is increasing evidence that AMPs shape the microbiota and the microbiota in turn modulate AMP expression. [7,8] For example, RNase 7 is a major human skin-derived AMP playing an important role in human cutaneous innate defense by controlling the growth of various microbes.

show abstract

“…3D printing can be used to create intricate architectures to aid with these shortages. 3D printing is an integrated approach to robotic fabrication, using computer-aided design (CAD) systems to deposit layers of biomaterials (within external anatomy, within internal anatomy and replacement parts for devices) [5][6][7][8][9][10]. The success of a medical device is not only dependent on the type of biomaterial used for its fabrication but also on the structural integrity and quality (defect free) of the printing parts.…”

mentioning

confidence: 99%

Use of 3D Printing to Support COVID-19 Medical Supply Shortages: A Review

Ishack

Lipner

2021

J. 3D Print. Med.

Self Cite

View full text Add to dashboard Cite

The novel coronavirus, COVID-19, created a pandemic with significant mortality and morbidity which poses challenges for patients and healthcare workers. The global spread of COVID-19 has resulted in shortages of personal protective equipment (PPE) leaving frontline health workers unprotected and overwhelming the healthcare system. 3D printing is well suited to address shortages of masks, face shields, testing kits and ventilators. In this article, we review 3D printing and suggest potential applications for creating PPE for healthcare workers treating COVID-19 patients. A comprehensive literature review was conducted using PubMed with keywords “Coronavirus disease 2019”, “COVID-19”, “severe acute respiratory syndrome coronavirus 2”, “SARS-CoV-2”, “supply shortages”, “N95 respirator masks”, “personal protective equipment”, “PPE”, “ventilators”, “three-dimensional model”, “three-dimensional printing” “3D printing” and “ventilator”. A summary of important studies relevant to the development of 3D printed clinical applications for COVID-19 is presented. 3D technology has great potential to revolutionize healthcare through accessibility, affordably and personalization.

show abstract

A Review of 3-Dimensional Skin Bioprinting Techniques: Applications, Approaches, and Trends

Cited by 25 publications

References 45 publications

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

Skin microbiota analysis in human 3D skin models—“Free your mice”

Use of 3D Printing to Support COVID-19 Medical Supply Shortages: A Review

Contact Info

Product

Resources

About