A review of a potential and promising probiotic candidate—
            <i>Akkermansia muciniphila</i>

Cheng, Dan; Xie, Meizhen

doi:10.1111/jam.14911

Cited by 82 publications

(62 citation statements)

References 65 publications

(121 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protective effects of Akk have long been verified to be due to its ability to degrade mucin in the gut and its competitive adhesion in intestinal epithelial cells against pathogenic bacteria, thus facilitating functions of the gut epithelial barrier and body homeostasis. 19 , 37 Increasing studies have suggested that these beneficial effects can also be attributed to surface components or metabolites. A specific outer membrane protein derived from Akk , Amuc_1100, has been shown to improve the gut barrier and metabolism in obese and diabetic mice.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages

Luo¹,

Xia²,

Liu³

et al. 2021

IJN

View full text Add to dashboard Cite

Purpose Prostate cancer (PCa) is one of the most common malignancies in males. Despite the success of immunotherapy in many malignant cancers, strategies are still needed to improve therapeutic efficacy in PCa. This study aimed to investigate the effects of Akkermansia muciniphila -derived extracellular vesicles ( Akk -EVs) on PCa and elucidate the underlying immune-related mechanism. Methods Akk -EVs were isolated by ultracentrifugation and intravenously injected to treat syngeneic PCa-bearing immune-competent mice. Immunophenotypic changes in immune cells, such as cytotoxic T lymphocytes and macrophages, were measured via flow cytometry analysis. Histological examination was used to detect morphological changes in major organs after Akk -EVs treatments. In vitro, flow cytometry was performed to confirm the effects of Akk -EVs on the activation of CD8 + T cells. Quantitative PCR and immunofluorescence staining were carried out to test the impact of Akk -EVs on macrophage polarization. Cell counting kit-8 (CCK-8) analysis, colony formation assays, and scratch wound healing assays were conducted to assess the effects of Akk -EVs-treated macrophages on the proliferation and invasion of PCa cells. CCK-8 assays also confirmed the impact of Akk -EVs on the viability of normal cells. Results Intravenous injection of Akk -EVs in immune-competent mice reduced the tumor burden of PCa without inducing obvious toxicity in normal tissues. This treatment elevated the proportion of granzyme B-positive (GZMB + ) and interferon γ-positive (IFN-γ + ) lymphocytes in CD8 + T cells and caused macrophage recruitment, with increased tumor-killing M1 macrophages and decreased immunosuppressive M2 macrophages. In vitro, Akk -EVs increased the number of GZMB + CD8 + and IFN-γ + CD8 + T cells and M1-like macrophages. In addition, conditioned medium from Akk -EVs-treated macrophages suppressed the proliferation and invasion of prostate cells. Furthermore, the effective dose of Akk -EVs was well-tolerated in normal cells. Conclusion Our study revealed the promising prospects of Akk -EVs as an efficient and biocompatible immunotherapeutic agent for PCa treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages

Luo¹,

Xia²,

Liu³

et al. 2021

IJN

View full text Add to dashboard Cite

show abstract

“…(2) Novel probiotics. A. muciniphila has recently become the new candidate for probiotics to improve a variety of diseases [ 83 – 85 ]. The abundance of A. muciniphila in T2DM patients is significantly reduced [ 86 ].…”

Section: Therapies For T2dm Based On Gut Microbiotamentioning

confidence: 99%

Gut Microbiota and Type 2 Diabetes Mellitus: Association, Mechanism, and Translational Applications

Zhang

Chu

Hao

et al. 2021

Mediators of Inflammation

View full text Add to dashboard Cite

Gut microbiota has attracted widespread attention due to its crucial role in disease pathophysiology, including type 2 diabetes mellitus (T2DM). Metabolites and bacterial components of gut microbiota affect the initiation and progression of T2DM by regulating inflammation, immunity, and metabolism. Short-chain fatty acids, secondary bile acid, imidazole propionate, branched-chain amino acids, and lipopolysaccharide are the main molecules related to T2DM. Many studies have investigated the role of gut microbiota in T2DM, particularly those butyrate-producing bacteria. Increasing evidence has demonstrated that fecal microbiota transplantation and probiotic capsules are useful strategies in preventing diabetes. In this review, we aim to elucidate the complex association between gut microbiota and T2DM inflammation, metabolism, and immune disorders, the underlying mechanisms, and translational applications of gut microbiota. This review will provide novel insight into developing individualized therapy for T2DM patients based on gut microbiota immunometabolism.

show abstract

“…One prominent bacterium that has wide pro-health effects is Akkermansia muciniphila (Akk). Akk is epidemiologically associated with the consumption of health-related food items, leanness, exercise, fitness and healthy aging [7,10,[12][13][14][15][16]. Its transfer into short-lived mouse strains extends longevity, supporting that Akk has an antiaging effect [17].…”

Section: Introductionmentioning

confidence: 99%

Oral administration of Akkermansia muciniphila elevates systemic antiaging and anticancer metabolites

Grajeda‐Iglesias

Durand

Daillère

et al. 2021

Aging

103

View full text Add to dashboard Cite

The presence of Akkermansia muciniphila (Akk) in the human gut is associated with good health, leanness and fitness. Mouse experimentation has demonstrated positive effects for Akk, which counteracts aging, mediates antiobesity and antidiabetic effects, dampens inflammation and improves anticancer immunosurveillance. Clinical trials have confirmed antidiabetic effects for Akk. Here, we investigated the time-dependent effects of oral administration of Akk (which was live or pasteurized) and other bacteria to mice on the metabolome of the ileum, colon, liver and blood plasma. Metabolomics was performed by a combination of chromatographic and mass spectrometric methods, yielding a total of 1.637.227 measurements. Akk had major effects on metabolism, causing an increase in spermidine and other polyamines in the gut and in the liver. Pasteurized Akk (Akk-past) was more efficient than live Akk in elevating the intestinal concentrations of polyamines, short-chain fatty acids, 2-hydroxybutyrate, as well multiple bile acids, which also increased in the circulation. All these metabolites have previously been associated with human health, providing a biochemical basis for the beneficial effects of Akk.

show abstract

A review of a potential and promising probiotic candidate— Akkermansia muciniphila

Abstract: Article type : JAM-Review Article A review of a potential and promising probiotic candidate-Akkermansia muciniphila

Cited by 82 publications

References 65 publications

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8+ T Cells and Macrophages

Gut Microbiota and Type 2 Diabetes Mellitus: Association, Mechanism, and Translational Applications

Oral administration of Akkermansia muciniphila elevates systemic antiaging and anticancer metabolites

Contact Info

Product

Resources

About