1The concentrations of six urinary monohydroxyl metabolites (OH-PAHs) of polycyclic 2 aromatic hydrocarbons, namely 1-hydroxynaphthalene, 1-hydroxyacenaphthene, 2-3 hydroxyfluorene, 1-hydroxyphenanthrene, 1-hydroxypyrene (1OHPy), and 3-4 hydroxybenzo[a]pyrene, were assessed in the post-shift urine of wildland firefighters involved 5 in fire combat activities at six Portuguese fire corporations, and compared with those of non-6 exposed subjects. Overall, median levels of urinary individual and total OH-PAHs (ΣOH-7 PAHs) suggest an increased exposure to polycyclic aromatic hydrocarbons during firefighting 8 activities with ΣOH-PAH levels in exposed firefighters 1.7 to 35 times higher than in non-9 exposed ones. Urinary 1-hydroxynaphthalene and/or 1-hydroxyacenapthene were the 10 predominant compounds, representing 63-98% of ΣOH-PAHs, followed by 2-11 hydroxyfluorene (1-17%), 1-hydroxyphenanthrene (1-13%), and 1OHPy (0.3-10%). A similar 12 profile was observed when gender discrimination was considered. Participation in fire combat 13 activities promoted an increase of the distribution percentage of 1-hydroxynaphthalene and 1-14 hydroxyacenaphthene, while contributions of 1-hydroxyphenanthrene and 1OHPy decreased. 15The detected urinary 1OHPy concentrations (1.73×10 -2 -0.152 µmol/mol creatinine in exposed 16 subjects versus 1.21×10 -2 -5.44×10 -2 µmol/mol creatinine in non-exposed individuals) were 17 lower than the benchmark level (0.5 µmol/mol creatinine) proposed by the American 18Conference of Governmental Industrial Hygienists. This compound, considered the biomarker 19 of exposure to PAHs, was the less abundant one from the six analyzed biomarkers. Thus the 20 inclusion of other metabolites, in addition to 1OHPy, in future studies is suggested to better 21 estimate firefighters' occupational exposure to PAHs. Moreover, strong to moderate 22 Spearman correlations were observed between individual compounds and ΣOH-PAHs 23 corroborating the prevalence of an emission source. 24 25