A review of algorithmic approaches for cell culture media optimization

Abstract

Cell culture media composition and culture conditions play a crucial role in product yield, quality and cost of production. Culture media optimization is the technique of improving media composition and culture conditions to achieve desired product outcomes. To achieve this, there have been many algorithmic methods proposed and used for culture media optimization in the literature. To help readers evaluate and decide on a method that best suits their specific application, we carried out a systematic review of the different methods from an algorithmic perspective that classifies, explains and compares the available methods. We also examine the trends and new developments in the area. This review provides recommendations to researchers regarding the suitable media optimization algorithm for their applications and we hope to also promote the development of new cell culture media optimization methods that are better suited to existing and upcoming challenges in this biotechnology field, which will be essential for more efficient production of various cell culture products.

Optimization of an Alternative Culture Medium for Phycocyanin Production from Arthrospira platensis under Laboratory Conditions

“…The coefficients with a positive sign in the adjusted model indicate that the variable can improve the response. On the contrary, a negative sign in the regression equation implies the variable’s capacity to reduce the response [ 34 ]. Equations (3) and (4) indicated that an increase in urea concentration negatively affects the growth of microalgal biomass.…”

Morphological, Physiological and Biochemical Characterization of Pseudoxanthomonas Species and Its Optimal Growth Kinetics

“…The composition of the culture medium is a significant parameter, and the components and their concentrations affect the growth of microorganisms and the accumulation of metabolites. Therefore, to achieve cost-effectiveness, it is necessary to optimize the composition and concentration of the medium based on a standardized medium to obtain the most suitable conditions for microorganism growth (Batista and Fernandes 2015;Zhou et al 2023).…”

Plant Growth Regulation in Cell and Tissue Culture In Vitro

“…New approaches using data-driven optimization of culture medium composition also show very relevant results [ 1 ], but it is shown in this communication that the directed use of current knowledge on classical plant physiology can result in comparatively rapid elucidation and marked improvement of culture techniques. Moreover, many of the presented results and observations are also based upon the practical experience of the authors, gained during more than 30 years of research in this field.…”