A review of attenuation correction techniques for tissue fluorescence

Advanced glycation end products (AGEs) are a complex and heterogeneous group of compounds that have been implicated in diabetes related compli¯cations. Skin auto°uorescence was recently introduced as an alternative tool for skin AGEs accumulation assessment in diabetes. Successful optical diagnosis of diabetes requires a rapid and accurate classi¯cation algorithm. In order to improve the performance of noninvasive and optical diagnosis of type 2 diabetes, support vector machines (SVM) algorithm was implemented for the classi¯cation of skin auto°uorescence from diabetics and control subjects. Cross-validation and grid-optimization methods were employed to calculate the optimal parameters that maximize classi¯cation accuracy. Classi¯cation model was set up according to the training set and then veri¯ed by the testing set. The results show that radical basis function is the best choice in the four common kernels in SVM. Moreover, a diagnostic accuracy of 82.61%, a sensitivity of 69.57%, and a speci¯city of 95.65% for discriminating diabetics from control subjects were achieved using a mixed kernel function, which is based on liner kernel function and radical basis function. In comparison with fasting plasma glucose and HbA 1c test, the classi¯cation method of skin auto°uorescence spectrum based on SVM shows great potential in screening of diabetes.

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“…It can be predicted that the development of the spectral correction technique 11 will promote the classi¯cation accuracy.…”

Section: Discussionmentioning

confidence: 99%

Classification of Skin Autofluorescence Spectrum Using Support Vector Machine in Type 2 Diabetes Screening

Zhang

Zhu

Wang

et al. 2013

J. Innov. Opt. Health Sci.

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“…We have found that subtracting the reflectance from the fluorescence or dividing the two parameters provides similar net NADH changes. Very recently, Bradley and Thorniley (20) published a review article dealing with the various correction techniques for tissue fluorescence. They summarized their review by the following conclusion: "even though research has been conducted into correction techniques for over thirty years, the development of a successful and practical correction technique remains a considerable challenge.…”

Section: Monitoring Devices and Technological Aspectsmentioning

confidence: 99%

Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Mayevsky¹,

Rogatsky²

2007

American Journal of Physiology-Cell Physiology

329

290

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Mayevsky A, Rogatsky GG. Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies. Am J Physiol Cell Physiol 292: C615-C640, 2007. First published August 30, 2006; doi:10.1152/ajpcell.00249.2006.-Normal mitochondrial function is a critical factor in maintaining cellular homeostasis in various organs of the body. Due to the involvement of mitochondrial dysfunction in many pathological states, the real-time in vivo monitoring of the mitochondrial metabolic state is crucially important. This type of monitoring in animal models as well as in patients provides real-time data that can help interpret experimental results or optimize patient treatment. The goals of the present review are the following: 1) to provide an historical overview of NADH fluorescence monitoring and its physiological significance; 2) to present the solid scientific ground underlying NADH fluorescence measurements based on published materials; 3) to provide the reader with basic information on the methodologies used in the past and the current state of the art fluorometers; and 4) to clarify the various factors affecting monitored signals, including artifacts. The large numbers of publications by different groups testify to the valuable information gathered in various experimental conditions. The monitoring of NADH levels in the tissue provides the most important information on the metabolic state of the mitochondria in terms of energy production and intracellular oxygen levels. Although NADH signals are not calibrated in absolute units, their trend monitoring is important for the interpretation of physiological or pathological situations. To understand tissue function better, the multiparametric approach has been developed where NADH serves as the key parameter. The development of new light sources in UV and visible spectra has led to the development of small compact units applicable in clinical conditions for better diagnosis of patients.real-time tissue viability; tissue spectroscopy; patient monitoring UNDERSTANDING THE MITOCHONDRIAL function has been a challenge for many investigators, including cytologists, biochemists, and physiologists, since its discovery more than 120 years ago. In addition to many books regarding the mitochondria, Ernster and Schatz (79) reviewed the history of mitochondrial structure and function studies. In the past two decades, several studies have reported mitochondrial involvement in pathological processes such as stroke (225) or cytoprotection (77). Most of the information on the mitochondrial function has been accumulated from in vitro studies. A relatively small portion of published papers dealt with the monitoring of mitochondrial function in vivo and in real time. Presently, examination of the involvement of the mitochondrial function in many pathological states, such as sepsis, requires monitoring of patients treated in intensive care units. Unfortunately, real-time monitoring of the mitochondrial function in patients has rarely been performed. The current study pres...

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“…We have found that subtracting the reflectance from the fluorescence or dividing fluorescence over reflectance provides similar net NADH changes. In 2006, Bradley and Thorniley 55 reviewed the available techniques for correction of fluorescence signals. They concluded, "even though research has been conducted into correction techniques for over thirty years, the development of a successful and practical correction technique remains a considerable challenge.…”

Section: Monitoring Of Nadh: History Principles and Basic Technologymentioning

confidence: 99%

Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system

et al. 2011

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Abstract. Background: The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients. Methods and Results: The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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A review of attenuation correction techniques for tissue fluorescence

Cited by 119 publications

References 86 publications

Classification of Skin Autofluorescence Spectrum Using Support Vector Machine in Type 2 Diabetes Screening

Classification of Skin Autofluorescence Spectrum Using Support Vector Machine in Type 2 Diabetes Screening

Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system

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