A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

Hong, Rui; Sun, Hongyu; Li, Dujuan; Yang, Weihuang; Fan, Kai; Liu, Chaoran; Dong, Linxi; Wang, Gaofeng

doi:10.3390/life12030342

Cited by 43 publications

(23 citation statements)

References 121 publications

(172 reference statements)

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“…The diffraction peaks at 2θ = 7.59 and 8.72° reflect the (110) and (002) planes of UiO-66, where 2θ = 12.26, 14.97, 17.28, 22.42, 25.94, and 30.93° represent UiO- 66 (022), (113), (004), (115), (224), and (046) planes. This is consistent with the typical XRD pattern of UiO-66. , Compared to the XRD spectrum of UiO-66, the XRD spectra of Co-UiO-66 are not significantly different, suggesting that the coverage of the Co layer has little effect on the crystal structure of UiO-66.…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

Zhang

et al. 2023

Anal. Chem.

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The ability to efficiently detect trace disease biomarkers in whole blood remains an enormous challenge.Researchers have paid more attention to the homogeneous electrochemical ratio biosensor due to its self-calibration capability and improved detection accuracy. However, proportional homogeneous electrochemical sensing is difficult to achieve and typically requires functional modification of the electrode or the preparation of complex materials. Herein, a dual-signal ratiometric aptamer homogeneous electrochemical microswimming detection device with active capture capability and one-step detection of human epidermal growth factor receptor-2 (HER2) is proposed. The homogeneous electrochemical biosensor is fabricated based on a functionalized nanocomposite double-stranded DNA({singlestranded DNA-ferrocene (Fc)-aptamer})@Co-UiO-66 with catalase properties and adsorptive properties to electroactive toluidine blue (TB) molecules. Encapsulation of Co-UiO-66 material with dsDNA (ssDNA-Fc-Apt) containing HER2 aptamer as a gate switch inhibited its ability to adsorb TB molecules. This functionalized Co-UiO-66 material can catalyze hydrogen peroxide. Using hydrogen peroxide as a fuel, it breaks down to release oxygen bubbles, creating a propulsion force that drives dsDNA(ssDNA-Fc-Apt)@Co-UiO-66 target HER2 through whole blood. When the surface dsDNA (ssDNA-Fc-Apt)@Co-UiO-66 recognizes HER2, a strand displacement reaction occurs, and the ssDNA-Fc is released into solution. The HER2 aptamer is coiled because it targets HER2, and the ability to adsorb TB molecules is restored due to the exposed surface of Co-UiO-66. A certain negative voltage is applied to the ITO electrode, and due to the electrostatic attraction, the TB molecules and ssDNA-Fc are adsorbed and enriched on the surface of the electrode by electrostatic attraction, which produces two strong and oppositely changing electrochemical signals, and the electrochemical signals depend on the HER2 concentration. It can sensitively detect HER2 biomarkers in only 40 min with the detection range of 0.0001−10 ng/mL and detection limits as low as 10 fg/mL. The electrochemical microswimmer for the detection of trace disease biomarkers involves a one-step process of capture, signal change, and detection.

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Section: Resultssupporting

confidence: 84%

“…This is consistent with the typical XRD pattern of UiO-66. 30,31 Compared to the XRD spectrum of UiO-66, the XRD spectra of Co-UiO-66 are not significantly different, suggesting that the coverage of the Co layer has little effect on the crystal structure of UiO-66.…”

Section: Resultsmentioning

confidence: 98%

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

Zhang

et al. 2023

Anal. Chem.

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“…The device modifies polypeptide-doped PEDOT onto the electrode surface, which can detect CA15-3 in serum without being affected by biofouling and has high sensitivity and long-term stability. The linear range is 0.01~1000 U/mL, and the detection limit is 3.34 U/mL [ 86 ].…”

Section: Application Of Biosensor In Detection Of Tumor Biomarkersmentioning

confidence: 99%

Recent Progress in Biosensors for Detection of Tumor Biomarkers

Jiang

Xue

et al. 2022

Molecules

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Cancer is a leading cause of death worldwide, with an increasing mortality rate over the past years. The early detection of cancer contributes to early diagnosis and subsequent treatment. How to detect early cancer has become one of the hot research directions of cancer. Tumor biomarkers, biochemical parameters for reflecting cancer occurrence and progression have caused much attention in cancer early detection. Due to high sensitivity, convenience and low cost, biosensors have been largely developed to detect tumor biomarkers. This review describes the application of various biosensors in detecting tumor markers. Firstly, several typical tumor makers, such as neuron-specific enolase (NSE), carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), squamous cell carcinoma antigen (SCCA), carbohydrate, antigen19-9 (CA19-9) and tumor suppressor p53 (TP53), which may be helpful for early cancer detection in the clinic, are briefly described. Then, various biosensors, mainly focusing on electrochemical biosensors, optical biosensors, photoelectrochemical biosensors, piezoelectric biosensors and aptamer sensors, are discussed. Specifically, the operation principles of biosensors, nanomaterials used in biosensors and the application of biosensors in tumor marker detection have been comprehensively reviewed and provided. Lastly, the challenges and prospects for developing effective biosensors for early cancer diagnosis are discussed.

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“…Liver cancers are associated with elevated mortality rates across the world, [1][2][3] and while significant advancements have been made in surgical techniques, chemotherapy and other treatment approaches, the 5-year survival rate remains far from satisfactory. [4][5][6] Moreover, liver cancer is the most common type of cancer in China. Specifically, cancer recurrence at the intermediate or advanced stage occurs in approx.…”

Section: Introductionmentioning

confidence: 99%