A review of cardiac troponin I detection by surface enhanced Raman spectroscopy: Under the spotlight of point-of-care testing

Saviñon-Flores, Anel I.; Saviñon-Flores, Fernanda; Trejo, G.; Méndez, Erika; Ţălu, Ștefan; González‐Fuentes, Miguel A.; Méndez‐Albores, Alia

doi:10.3389/fchem.2022.1017305

Cited by 17 publications

(13 citation statements)

References 242 publications

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“…UA and cTn are typical biomarkers for gout and AMI, respectively. [42][43][44][45][46][47] However, the goldstandard methods used in clinic cannot be used to give rapid results in the home. Together with a homemade centrifugal device and hand-held Raman spectrometer, the 3D-graphene/Al-AAO substrate was used to create a home detection system to rapidly detect the amount of UA and cTn in the blood of a patient.…”

Section: Resultsmentioning

confidence: 99%

A synergistic biomimetic optical structure for household health monitoring

Zhang

Ding

et al. 2023

Preprint

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A breakthrough in the performance of bionic optical structures will only be achieved if we can obtain an in-depth understanding of the synergy mechanisms operating in natural optical structures and find ways to imitate them. In this work, inspired by feline eyes, an optical structure that takes advantage of a synergistic effect that occurs between resonant and reflective structures was designed. The reflective structure consists of anodic aluminum oxide with an aluminized inner layer (Al-AAO), and the resonant structure consists of three-dimensional (3D) graphene inside, and on the surface of the Al-AAO. The synergistic effect between the reflective and resonant components leads to a Raman enhancement factor (EF) of 1.16 × 107 which is much greater than that achieved using the reflective/resonant cavities on their own. A 2-3 order of magnitude increase in sensitivity could thus be achieved when used to detect model compounds. More importantly, the optical device was further used to develop a highly-sensitive household health monitoring system. The system uses simple apparatus (homemade centrifugal device and hand-held Raman spectrometer) and rapidly produces results (detection time＜3 min). It can thus be used to give early warning of acute diseases with high risk (e.g., acute myocardial infarction). The 3D-graphene/Al-AAO substrates were also found to have good reusability and storability (9% and 7% reduction in EF after washing 30 times and 8 weeks of storage, respectively). They thus reduce detection costs (to ~$1), making them much cheaper to use than the current gold-standard methods (e.g., ~$16 for gout detection).

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Section: Resultsmentioning

confidence: 99%

A synergistic biomimetic optical structure for household health monitoring

Zhang

Ding

et al. 2023

Preprint

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“…An important advantage of modern highly sensitive troponin immunotests is the ability to detect cardiac troponin molecules in noninvasively obtained biological material (oral fluid, urine, sweat) [ 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ]. When receiving these biomaterials, a number of advantages can be noted: the process is non-invasive, atraumatic, and painless, no specially trained medical personnel is required, and the preliminary diagnostics can be tested at home (test strips).…”

Section: Characteristics and Benefits Of Using New Highly Sensitive L...mentioning

confidence: 99%

Gender Specificities of Cardiac Troponin Serum Levels: From Formation Mechanisms to the Diagnostic Role in Case of Acute Coronary Syndrome

Chaulin

2023

Life

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Cardiac troponins T and I are the main (most sensitive and specific) laboratory indicators of myocardial cell damage. A combination of laboratory signs of myocardial cell damage (elevated levels of cardiac troponins T and I) with clinical (severe chest pain spreading to the left side of the human body) and functional (rise or depression of the ST segment, negative T wave or emergence of the Q wave according to electrocardiography and/or decrease in the contractility of myocardial areas exposed to ischemia according to echocardiography) signs of myocardial ischemia is indicative of the ischemic damage to cardiomyocytes, which is characteristic of the development of acute coronary syndrome (ACS). Today, with early diagnostic algorithms for ACS, doctors rely on the threshold levels of cardiac troponins (99th percentile) and on the dynamic changes in the serum levels over several hours (one, two, or three) from the moment of admission to the emergency department. That said, some recently approved highly sensitive methods for determining troponins T and I show variations in 99th percentile reference levels, depending on gender. To date, there are conflicting data on the role of gender specificities in the serum levels of cardiac troponins T and I in the diagnostics of ACS, and the specific mechanisms for the formation of gender differences in the serum levels of cardiac troponins T and I are unknown. The purpose of this article is to analyze the role of gender specificities in cardiac troponins T and I in the diagnostics of ACS, and to suggest the most likely mechanisms for the formation of differences in the serum levels of cardiac troponins in men and women.

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“…More recently in 2018, the acronym has been extended to REASSURED, which considers real-time connectivity, ease of specimen collection, affordable, sensitive, specific, user-friendly, rapid, robust, equipment free and environmentally friendly, and deliverable to end-users. 2,3 The diagnostic device development criteria are focused on finding a balance between accuracy, affordability, and accessibility to provide care across a variety of healthcare system settings. 3 Cardiovascular disease continues to be the leading cause of death across the globe 4 ; however, innovative POC platforms have the potential to improve the detection and diagnosis of these diseases in both underserved and developed healthcare settings.…”

Section: Introductionmentioning

confidence: 99%

“…5,13,14 Clinical laboratory tests for cTnI are categorized by their sensitivity, ranging from conventional (0-0.04 ng/mL) to ultra-high sensitivity (<0.014 ng/mL), and typically have a turnaround time of one day or more; whereas commercial POC tests are designed to be rapid, on the order of 20 min. 2,3,[15][16][17] However, many current POC platforms are limited due to their reduced detection sensitivity and precision when compared to clinical alternatives. 2,6,18 The technologies enabling POC detection of cardiac biomarkers have continued to improve detection sensitivity and usability.…”

Section: Introductionmentioning

confidence: 99%

“…2,3,[15][16][17] However, many current POC platforms are limited due to their reduced detection sensitivity and precision when compared to clinical alternatives. 2,6,18 The technologies enabling POC detection of cardiac biomarkers have continued to improve detection sensitivity and usability. Various immunoassay platforms have been developed to include an array of signal transduction methods ranging from fluorescence, electrochemistry, surface plasmon resonance, and surface-enhanced Raman scattering (SERS).…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Clinical Utility of Raman Spectroscopy for Point-of-Care Cardiovascular Disease Biomarker Detection

Soliman

Mabbott

et al. 2023

Appl Spectrosc

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A variety of innovative point-of-care (POC) solutions using Raman systems have been explored. However, the vast effort is in assay development, while studies of the characteristics required for Raman spectrometers to function in POC applications are lacking. In this study, we tested and compared the performance of eight commercial Raman spectrometers ranging in size from benchtop Raman microscopes to portable and handheld Raman spectrometers using paper fluidic cartridges, including their ability to detect cardiac troponin I and heart fatty acid binding protein, both of which are well-established biomarkers for evaluating cardiovascular health. Each spectrometer was evaluated in terms of excitation wavelength, laser characteristics, and ease of use to investigate POC utility. We found that the Raman spectrometers equipped with 780 and 785 nm laser sources exhibited a reduced background signal and provided higher sensitivity compared to those with 633 and 638 nm laser sources. Furthermore, the spectrometer equipped with the single acquisition line readout functionality showed improved performance when compared to the point scan spectrometers and allowed measurements to be made faster and easier. The portable and handheld spectrometers also showed similar detection sensitivity to the gold standard instrument. Lastly, we reduced the laser power for the spectrometer with single acquisition line readout capability to explore the system performance at a laser power that change the classification from a Class 3B laser device to a Class 3R device and found that it showed comparable performance. Overall, these findings show that portable Raman spectrometers have the potential to be used in POC settings with accuracy comparable to laboratory-grade instruments, are relatively low-cost, provide fast signal readout, are easy to use, and can facilitate access for underserved communities.

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A review of cardiac troponin I detection by surface enhanced Raman spectroscopy: Under the spotlight of point-of-care testing

Cited by 17 publications

References 242 publications

A synergistic biomimetic optical structure for household health monitoring

A synergistic biomimetic optical structure for household health monitoring

Gender Specificities of Cardiac Troponin Serum Levels: From Formation Mechanisms to the Diagnostic Role in Case of Acute Coronary Syndrome

Exploring the Clinical Utility of Raman Spectroscopy for Point-of-Care Cardiovascular Disease Biomarker Detection

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