A review of CRISPR Cas9 for ASCVD: treatment strategies and could target PSCK9 gene using CRISPR cas9 prevent the patient from atherosclerotic vascular disease?

Abstract

Background: Targeting PCSK9 by maintaining hemodynamic shear stress stability has been shown in several studies to reduce LDL-C, arterial plaque formation, and PCSK9 expression in atherosclerotic cardiovascular disease. Genome editing with CRISPR-associated regularly interspersed short palindromic repeats (CRISPR/Cas9) have therapeutic potential for atherosclerotic cardiovascular disease. This study aims to evaluate the role of CRISPR/Cas9 in targeting PCSK9 as an effective therapeutic and long-term effect on atherosclerotic vascular disease. Methods: The method used in this study summarizes the article by looking for keywords that have been determined in the title and abstract. The authors used official guidelines from Science Direct, PubMed, the American College of Cardiology, Google Scholar, and PERKI to select full-text articles published within the last decade, prioritizing searches within the last five years. Results: CRISPR/Cas9 deletion of PCSK9 in mouse models reduces LDL-C, Plaque accumulation in the arteries, and PCSK9 expression. Furthermore, CRISPR/Cas9 deletion in PCSK9 saves the stability of Hemodyanimc shear stress to control the PCSK9 expression that causes Atherosclerotic cardiovascular disease. Conclusion: PCSK9 targeting by CRISPR/Cas9 deletion effectively reduces LDL-C, plaque buildup in the arteries, and PCSK9 expression. However, more research is needed to determine its side effects and safety.

CRISPR-Cas9 Genomic Editing as an Innovation in the Management of Sickle Cell Disease: A Systematic Review

“…2(2) 44-56, 2023 the subject matter (considering the fact this technology is novel) and its stage of development is still in the clinical trials. A similar method of literature search as described by Suwito, et al, 2023was used (Suwito, 2023. The literature search was done from the following databases over a period of 2 weeks: PubMed (mostly used), Google Scholar, and African Journals Online (AJOL) using the following terms: CRISPR-Cas9, genomic editing, gene editing, sickle cell disease, hemoglobinopathies, sickle cell anemia, genetic therapy, systematic review, new therapy/novel intervention for sickle cell disease cure/treatment.…”

Application of CRISPR-Cas9 genome editing technology in various fields: A review

“…The controversy surrounding CRISPR-Cas9 has spurred broader discussions on the ethical, legal, and social implications (ELSI) of gene editing technologies [ 63 ]. These discussions encompass topics such as equitable access to CRISPR-based therapies, potential uses for enhancement rather than therapeutic purposes, and the importance of public engagement and involvement in decision-making processes [ 64 , 65 ]. International conferences and expert panels have been convened to address these ELSI concerns and provide guidelines for responsible and ethical use of CRISPR-Cas9 technology.…”