A review of DNA profiling success for laser microdissected forensic casework samples

Vintiner, Sue; Veth, Johanna; Bright, Jo‐Anne

doi:10.1080/00450618.2018.1510030

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…It should also be noted that inspection of the sperm morphology (head and tail shape or length, which may be a requirement in some laboratory protocols) via bright field or phase contrast microscopy is not readily achievable due to the interference from pores on the membrane slide. Despite these limitations, LMD may be particularly useful in challenging cases whereby small numbers of sperm are present in samples containing large numbers of epithelial cells (Vintiner, Veth, & Bright, 2018). Following staining of the sperm with the Sperm Hy‐Liter Express kit, this technique has been reported to successfully generate high quality genotyping results with only 50 laser microdissected sperm (Costa, Correia‐de‐Sá, Porto, & Cainé, 2017).…”

Section: Isolation Of Male Dna In Sexual Assault Cases—alternatives Amentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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The extraction of DNA is a fairly standard procedure routinely undertaken in biological academic and research settings. In a forensic DNA context, DNA extraction is the crucial first step toward the generation of DNA profiles used to support convictions or exonerations in the criminal justice system. However, it is often complicated by the additional challenge of yielding sufficient quantities of clean DNA that is free from environmental inhibitors, from a wide spectrum of sample types which may contain limited quantities of biological material. While improvements in methods and technology over the decades have sped up and simplified the process of DNA extraction, several issues remain to be fully addressed—such as the incomplete separation of spermic DNA from sperm‐epithelial cell mixtures, increasing demands for ever‐faster DNA results, or even on‐scene DNA processing, and processing of difficult samples such as skeletal remains or samples contaminated by Chemical, Biological, or Radiological (CBR) agents. These challenges are often compounded by high work volumes and limited quantities of starting material. As such, this review focuses on recent trends and developments in four areas of forensic DNA extraction: (a) analysis of sexual assault evidence which often represents a significant portion of a forensic DNA laboratory's casework, (b) use of portable rapid DNA instruments which has gained much traction among law enforcement in recent times, (c) DNA extraction from difficult and CBR‐contaminated samples which, albeit rare, would be essential in a DNA laboratory's toolbox in the event of an incident, and (d) bypassing DNA extraction altogether. This article is categorized under: Forensic Biology > Forensic DNA Technologies

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Section: Isolation Of Male Dna In Sexual Assault Cases—alternatives Amentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

View full text Add to dashboard Cite

show abstract

“… 58 LCM (sometimes referred to as LCD) utilizes a light microscope and UV laser to cut out the specific cell or cells of interest. Subsequent studies have demonstrated further success with LCM 59 , 60 , 61 , 62 even utilizing LCM in conjunction with chromosome specific probes. 63 , 64 PG approaches have also been applied to laser captured cells resulting in cell population subsamples (10x 20-cell subsamples) with various weight ratios resulting in the recovery of more genotype information as opposed to standard DNA extractions.…”

Section: Cell Capturementioning

confidence: 99%