A review of ecological risk assessment methods for amphibians: Comparative assessment of testing methodologies and available data

Johnson, Mark S.; Aubee, Catherine; Salice, Christopher J.; Leigh, Katrina; Liu, Elissa; Pott, Ute; Pillard, David A.

doi:10.1002/ieam.1881

Cited by 35 publications

(22 citation statements)

References 72 publications

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“…In particular, for the 96‐hour acute experiments, we used a GLMM with a binomial distribution and logit link function (R package lme4; Bates, Maechler, Bolker, & Walker, ) to evaluate the effects of solvent treatment on the survival and occurrence of abnormalities. We then determined if solvent treatment influenced interorbital distance and mouth size using GLMM with a Gaussian distribution and log link function, including survival as a fixed effect to account for density effects (Johnson et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Specifically, we used a GLMM with a binomial distribution and logit link function (R package lme4; Bates et al, ) to evaluate solvent effects on the proportion of females to males. For the remaining traits, we included survival as a fixed effect (Johnson et al, ), and used a GLMM with log link (to prevent negative terms in the model; R packages lme4 (Bates et al, ) and lmerTest (Kuznetsova, Brockhoff, & Haubo Bojesen, ) and Gaussian distribution. For GS (considered count data), overdispersion was detected (dispersion value >1; Zuur, Hilbe, & Ieno, ) and was corrected by re‐fitting the model with a Poisson distribution with the glmmPQL function from the MASS package (Venables & Ripley, ) in R (reduced dispersion to 1.01).…”

Section: Methodsmentioning

confidence: 99%

“…We then determined if solvent treatment influenced interorbital distance and mouth size using GLMM with a Gaussian distribution and log link function, including survival as a fixed effect to account for density effects (Johnson et al, 2017).…”

Section: Statistical Analysesmentioning

confidence: 99%

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Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Young

Gavel

Gutierrez-Villagómez

et al. 2019

J of Applied Toxicology

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Carrier solvents are used frequently in toxicity testing to assist hydrophobic chemicals into solution, but such solvents may have toxic effects on test subjects. Amphibians are model organisms in toxicity studies; however, little is known about the direct effects of solvents on native amphibians. Following modifications to standardized guidelines for native species, we used acute 96‐hour exposures to assess the direct effects of three common solvents on survival, differences in morphology and occurrence of abnormalities of northern leopard frog larvae (Lithobates pipiens). The solvents, dimethyl sulfoxide (DMSO), ethanol (ETOH) and acetone (ACE) were used at nominal concentrations ranging from 1 to 100 μL/L. We also conducted a 30‐day exposure to assess the direct chronic effects of DMSO at 1 and 5 μL/L, on larval growth, development and sex differentiation, but found no effects. Acute exposure to solvents also had no effect on the survival of larvae, but we found significant abnormalities in tadpoles acutely exposed to 100 μL/L ACE. Acute exposure to DMSO and ETOH had further concentration‐dependent effects on larval morphological traits. Our study suggests that DMSO and ETOH at ≤20 μL/L may be used as solvents in amphibian ecotoxicological studies, but ACE should be limited to ≤50 μL/L in ecotoxicity studies and perhaps much less (≤10 μL/L) in studies with other amphibians, based on a review of existing literature. We emphasize pilot studies when using solvents on acute and chronic ecotoxicity tests, using native amphibians.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Young

Gavel

Gutierrez-Villagómez

et al. 2019

J of Applied Toxicology

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“…Amphibians appear to be an important test species for HPT axis interactions. Johnson et al (2017) stated that data for long‐term exposures and metamorphosis suggest that amphibians are more sensitive than fish and other vertebrates to compounds that affect the HPT axis, citing Kerby et al (2010) and Veldhoen et al (2006). Veldhoen et al (2006) found that triclosan altered thyroid hormone–associated gene expression, increased hindlimb development, and decreased total body weight.…”

Section: Biological Read‐across For Eatsmentioning

confidence: 99%

Critical Review of Read‐Across Potential in Testing for Endocrine‐Related Effects in Vertebrate Ecological Receptors

McArdle

Freeman

Staveley

et al. 2020

Enviro Toxic and Chemistry

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Recent regulatory testing programs have been designed to evaluate whether a chemical has the potential to interact with the endocrine system and could cause adverse effects. Some endocrine pathways are highly conserved among vertebrates, providing a potential to extrapolate data generated for one vertebrate taxonomic group to others (i.e., biological read‐across). To assess the potential for biological read‐across, we reviewed tools and approaches that support species extrapolation for fish, amphibians, birds, and reptiles. For each of the estrogen, androgen, thyroid, and steroidogenesis (EATS) pathways, we considered the pathway conservation across species and the responses of endocrine‐sensitive endpoints. The available data show a high degree of confidence in the conservation of the hypothalamus–pituitary–gonadal axis between fish and mammals and the hypothalamus–pituitary–thyroid axis between amphibians and mammals. Comparatively, there is less empirical evidence for the conservation of other EATS pathways between other taxonomic groups, but this may be due to limited data. Although more information on sensitive pathways and endpoints would be useful, current developments in the use of molecular target sequencing similarity tools and thoughtful application of the adverse outcome pathway concept show promise for further advancement of read‐across approaches for testing EATS pathways in vertebrate ecological receptors. Environ Toxicol Chem 2020;39:739–753. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

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“…Because of their varied habitat, life‐histories, and vulnerability to multiple environmental stressors, assessment of chemical risk to amphibians should be placed in a larger, ecologically relevant context (Johnson et al ). Amphibian life cycle and ontogeny are distinct from other taxonomic classes and vary widely between orders.…”

Section: Introductionmentioning

confidence: 99%

Guidance for Developing Amphibian Population Models for Ecological Risk Assessment

Awkerman

Raimondo

Schmolke

et al. 2019

Integr Envir Assess & Manag

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Despite widespread acceptance of the utility of population modeling and advocacy of this approach for a more ecologically relevant perspective, it is not routinely incorporated in ecological risk assessments (ERA). A systematic framework for situation‐specific model development is one of the major challenges to broadly adopting population models in ERA. As risk assessors confront the multitude of species and chemicals requiring evaluation, an adaptable stepwise guide for model parameterization would facilitate this process. Additional guidance on interpretation of model output and evaluating uncertainty would further contribute to establishing consensus on good modeling practices. We build on previous work that created a framework and decision guide for developing population models for ERA by focusing on data types, model structure, and extrinsic stressors relevant to anuran amphibians. Anurans have a unique life cycle with varying habitat requirements and high phenotypic plasticity. These species belong to the amphibian class, which is facing global population decline in large part due to anthropogenic stressors, including chemicals. We synthesize information from databases and literature relevant to amphibian risks to identify traits that influence exposure likelihood, inherent sensitivity, population vulnerability, and environmental constraints. We link these concerns with relevant population modeling methods and structure in order to evaluate pesticide effects with appropriate scale and parameterization. A standardized population modeling approach, with additional guidance for anuran ERA, offers an example method for quantifying population risks and evaluating long‐term impacts of chemical stressors to populations. Integr Environ Assess Manag 2020;16:223–233. © 2019 SETAC

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A review of ecological risk assessment methods for amphibians: Comparative assessment of testing methodologies and available data

Cited by 35 publications

References 72 publications

Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Assessment of sublethal ecotoxicity of solvents on larvae of a model native amphibian (Lithobates pipiens)

Critical Review of Read‐Across Potential in Testing for Endocrine‐Related Effects in Vertebrate Ecological Receptors

Guidance for Developing Amphibian Population Models for Ecological Risk Assessment

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