The visual search performance of radiologists is systematically challenged due to the use of flat-panel displays when viewing multi-slice images. Three-dimensional visualization of information is expected to facilitate visual search for radiologists. However, as the differences in user performance, depending on radiologist experience, have not yet been assessed for real three-dimensional images, it is difficult to estimate the usability of new visualization systems for radiologists with different amounts of professional search experience. In this study, we aimed to determine how three-dimensional visual search is affected by radiologist experience and the physical properties of stimuli. Radiologists, residents, and medical students accomplished visual search tasks with varying set sizes and target-distractor similarities. Search items were presented on multiple display planes and image manipulation was conducted using a computer keyboard. We compared the search outcome and strategy among three groups of participants. All three groups submitted a similar number of correct responses depending on target-distractor similarity. However, in contrast to radiologists and residents, it took a considerably longer time for students to find the targets. The major differences were evident in search strategy when comparing the image manipulations of radiologists with those of residents and students. Our results demonstrated that experienced radiologists’ search performance benefited from seeing the entire three-dimensional view of an image, whereas residents and students focused instead on reducing the amount of information and searched in a selective manner. These findings should be taken into account when planning the implementation of new workstations with three-dimensional visualization in radiology.