A review of fraction collection technology for supercritical fluid chromatography

Kaplitz, Alexander S.; Berger, Terry A.; Berger, Blair K.; Schug, Kevin A.

doi:10.1016/j.trac.2022.116588

Cited by 13 publications

(5 citation statements)

References 62 publications

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“…In recent years, there have been at least three excellent reviews highlighting the advantages of SFC for the analysis and purification of NPs (Hartmann and Ganzera 2015), including plants used in traditional Chinese medicine (Eisath et al 2018;Huang et al 2018). Last but not least, SFC is also considered today as the best strategy for chiral separation at the analytical scale, and above all preparative scale, using polysaccharide-based stationary phases (De Klerck et al 2012;Kaplitz et al 2022;West 2019).…”

Section: Green and Sustainable Preparative Lcmentioning

confidence: 99%

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

Queiroz,

Guillarme,

Wolfender

2024

Phytochem Rev

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The isolation of pure compounds from extracts represents a key step common to all investigations of natural product (NP) research. Isolation methods have gone through a remarkable evolution. Current approaches combine powerful metabolite profiling methods for compounds annotation with omics mining results and/or bioassay for bioactive NPs/biomarkers priorisation. Targeted isolation of prioritized NPs is performed using high-resolution chromatographic methods that closely match those used for analytical profiling. Considerable progress has been made by the introduction of innovative stationary phases providing remarkable selectivity for efficient NPs isolation. Today, efficient separation conditions determined at the analytical scale using high- or ultra-high-performance liquid chromatography can be optimized via HPLC modelling software and efficiently transferred to the semi-preparative scale by chromatographic calculation. This ensures similar selectivity at both the analytical and preparative scales and provides a precise separation prediction. High-resolution conditions at the preparative scale can notably be granted using optimized sample preparation and dry load sample introduction. Monitoring by ultraviolet, mass spectrometry, and or universal systems such as evaporative light scattering detectors and nuclear magnetic resonance allows to precisely guide the isolation or trigger the collection of specific NPs with different structural scaffolds. Such approaches can be applied at different scales depending on the amounts of NPs to be isolated. This review will showcase recent research to highlight both the potential and constraints of using these cutting-edge technologies for the isolation of plant and microorganism metabolites. Several strategies involving their application will be examined and critically discussed. Graphical abstract

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Section: Green and Sustainable Preparative Lcmentioning

confidence: 99%

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

Queiroz,

Guillarme,

Wolfender

2024

Phytochem Rev

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“…Significant progress is also being made in supercritical fluid chromatography (SFC)-MS purification . This technique has been used for the successful purification of synthesized compound libraries .…”

Section: Scalementioning

confidence: 99%

“…Significant progress is also being made in supercritical fluid chromatography (SFC)-MS purification. 9 This technique has been used for the successful purification of synthesized compound libraries. 10 and prep-SFC has found comparable success rates; 11 however, for most applications prep-LCMS, alone, is easier to setup and maintain.…”

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confidence: 99%

High-Throughput Purification in Drug Discovery: Scaling New Heights of Productivity

Jones

Goodyear

2023

ACS Med. Chem. Lett.

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With the “low hanging fruit” of early drug discovery gone, pharmaceutical companies are increasingly turning to developing high-throughput synthetic platforms capable of greatly shortening the design–make–test cycle of new drugs. Purification has long been considered the bottleneck of this procedure; however, new technologies and systems are now being integrated into these high-throughput synthetic workflows, providing compounds of high purity capable of being used directly in biological screening.

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“…In this vein, proteomics becomes an indispensable tool for studying carcinogenesis and other pathological processes. [1][2][3][4] Owing to their convenience and efficiency, various separation methods have been introduced for protein enrichment, including high performance liquid chromatography (HPLC), 5 capillary electrophoresis, 6 supercritical CO 2 fractionation technology, [7][8][9][10][11] etc.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resin composites for the enrichment of proteins, peptides and phosphopeptides

Wang¹,

Pan²,

Yan³

et al. 2023

Anal. Methods

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A review of fraction collection technology for supercritical fluid chromatography

Cited by 13 publications

References 62 publications

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

High-Throughput Purification in Drug Discovery: Scaling New Heights of Productivity

Magnetic resin composites for the enrichment of proteins, peptides and phosphopeptides

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