A review of high throughput technology for the screening of natural products

Mishra, K.P.; Ganju, Lilly; Sairam, M.; Banerjee, P. K.; Sawhney, R. C.

doi:10.1016/j.biopha.2007.06.012

Cited by 117 publications

(76 citation statements)

References 21 publications

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“…The search for new drugs of potential clinical interest directed to specific pathologically relevant targets is usually conducted through the analysis of the effect of hundreds to thousands of potential compounds, under many different possible conditions, in tests carried out under automated or semi-automated regimes (43)(44)(45). Our fluorescent plate reader assay has demonstrated its utility to evaluate surfactant inhibition effects such as those produced by serum proteins like albumin (8,46).…”

Section: Discussionmentioning

confidence: 99%

High-throughput evaluation of pulmonary surfactant adsorption and surface film formation

Ravasio

Cruz

Pérez‐Gil

et al. 2008

Journal of Lipid Research

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The assessment of new therapeutic strategies to cure surfactant-associated lung disorders would greatly benefit from assay systems allowing routine evaluations of surfactant functions. We present a method to measure surfactant adsorption kinetics into interfacial air-liquid interfaces based on fluorescence microplate readers. The principle of measurement is simple, robust, and reproducible: Wells of a microtiter plate contain an aqueous solution of a light-absorbing agent. Fluorescence is excited and collected from the top of the wells so that fluorescently labeled surfactant injected into the bulk can be detected only once adsorbed into the air-liquid interface. Mass transfer from the bulk to the interface is achieved by orbital shaking implemented in the plate reader instrument. The method has been tested and validated by using phospholipids or surfactants of different origins, by using albumin as surfactant inhibitor, and by comparison of results with Wilhelmy balance measurements. The method is suited for implementation in high-throughput screening routines for conditions affecting, or improving, surfactant film formation. In contrast to surface tension measurements, our method gives a direct readout of the amount of surfactant adsorbing into the interface, including the functionally important amount of material firmly associating with the interfacial film.-Ravasio, A., A. Cruz, J. Pérez-Gil, and T. Haller. High-throughput evaluation of pulmonary surfactant adsorption and surface film formation. J. Lipid Res. 2008Res. . 49: 2479Res. -2488

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Section: Discussionmentioning

confidence: 99%

High-throughput evaluation of pulmonary surfactant adsorption and surface film formation

Ravasio

Cruz

Pérez‐Gil

et al. 2008

Journal of Lipid Research

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“…Bioactive properties of various plants are connected with the presence of phenolic compounds, especially flavonoids (Mishra et al, 2008). The biological, pharmacological and medicinal properties of this group of compounds have been extensively reviewed (Marchand, 2002) and related to their antioxidant properties by preventing UV induced oxygen free radical generation and lipid peroxidation (Kaur and Saraf, 2011).…”

Section: Introductionmentioning

confidence: 99%

Exploring the antioxidant potential of Helichrysum stoechas (L.) Moench phenolic compounds for cosmetic applications: Chemical characterization, microencapsulation and incorporation into a moisturizer

Barroso

Barros

Dueñas

et al. 2014

Industrial Crops and Products

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The present work explores the antioxidant potential of Helichrysum stoechas (L.) Moench phenolic compounds for cosmetic applications involving the following steps: chemical characterization, microencapsulation and incorporation into a moisturizer.Eighteen different phenolic compounds were identified in flowering aerial parts (decoction and hydroalcoholic extract), being 3,5-O-dicaffeoylquinic acid and myricetin O-acetylhexoside the most abundant phenolic acid and flavonoid, respectively.Comparatively to the decoction form, the hydroalcoholic extract presented both higher antioxidant activity and higher phenolic content, being its lyophilized form chosen to

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“…[25][26][27][28] These libraries include subsections of crude extracts, pre-fractionated extracts and purified natural products. 29 A research group in Ireland has developed a two-dimensional chromatographic strategy that includes an automated HPLC-MS fractionation protocol to generate purified marine natural product libraries that are accurately characterized by mass during production to expedite dereplication of known compounds and identification of novel chemotypes.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting microbial natural product libraries from the marine environment for drug discovery

Ashforth

Ren

et al. 2010

J Antibiot

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Marine microorganisms are fascinating resources due to their production of novel natural products with antimicrobial activities. Increases in both the number of new chemical entities found and the substantiation of indigenous marine actinobacteria present a fundamental difficulty in the future discovery of novel antimicrobials, namely dereplication of those compounds already discovered. This review will share our experience on the taxonomic-based construction of a highly diversified and low redundant marine microbial natural product library for high-throughput antibiotic screening. We anticipate that libraries such as these can drive the drug discovery process now and in the future.

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A review of high throughput technology for the screening of natural products

Cited by 117 publications

References 21 publications

High-throughput evaluation of pulmonary surfactant adsorption and surface film formation

High-throughput evaluation of pulmonary surfactant adsorption and surface film formation

Exploring the antioxidant potential of Helichrysum stoechas (L.) Moench phenolic compounds for cosmetic applications: Chemical characterization, microencapsulation and incorporation into a moisturizer

Bioprospecting microbial natural product libraries from the marine environment for drug discovery

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