Low-intensity noncoherent luminescent radiation stimulates reparative processes in soft tissue wounds of rats. The stimulation is dependent on the frequency of fight pulsation and the luminescence spectrum.Key Words: luminescent radiation; laser radiation; phototherapy, soft tissue wounds Special fiber-optic sources of luminescent fight with a relatively broad spectral range (50-100 nm) have recently been developed [6]. In contrast to monochromatic laser radiation, these sources provide monochromatized radiation or, taking into account the mechanism of its creation and its specific features, luminescent monochromatized noncoherent radiation (LMNR) [7]. LMNR is not subject to the restrictions imposed on the premises and work regime of personnel operating laser phototherapeutic apparatus [8]. In light of the findings confmnmg the absence of a specific influence of lasers on biological objects [7,10], the replacement of laser fight in phototherapeutic procedures [2-4,9] by noncoherent broad-band radiation seems expedient.
MATERIALS AND METHODSThe sources of low-intensity noncoherent radiation were based on two-layer optical fibers with a transparent shell of methylmethacrylate and butylmethacrylate copolymer with a polystyrene core containing the photoluminescent additives phenalemine-439 and phenalemine-160), and on optical fibers made from fluorine-substituted methylmethacrylate with a methylmethacrylate core with rhodamine 6g-chloride. These additives enabled us to obtain three Medical Institute, Nizhnii Novgorod. (Presented by B. A. Korolev, Member of the Russian Academy ol Medical Sciences) different LMNR: red (600-680 nm, spectral maximum 635 urn), orange (600-680 nan, spectral maximum 605 nm), and green (500-580 nm, spectral maximum 540 nm).Experiments were performed on rats of the same fitter after a 10-day quarantine period during which the animals were kept in individual cages under the same conditions. Rats with signs of spontaneous pathology were not included in the study. Wounds were produced with a scalpel on the back (5-era cuts limited by the fascia were made along the body axis) under local Novocain (0.25%) anesthesia. The wound was closed immediately with three silk sutures placed at a distance of 1 cm from each other. The operation field was pretreated with 5% iodine [1].The reparative processes were controlled by determining scar fu'mness after complete epithelization by the method of wound tensiometry [2]. In addition, visual and histological studies of the scars were performed on days 3, 5, 7, and 10 of wound modeling.The treatment sessions with LMNR were started the day after the operation. An optical polymer fiber 0.8 mm in diameter was used. The distance between the distal end of the fiber and the tissue was 1-2 mm; exposure time was 15 rnin. The power density was 20 mW/cm :. The procedure was performed every day during a 5-day period. The ardmals were euthanized on days 5 and 7 by ether overdose. Tissue samples with scars