Abstract. FO-DTS (Fiber Optic Distributed Temperature Sensing) technology has been widely developed to quantify exchanges between groundwater and surface water during the last decade. In this study, we propose, for the first time, to combine long-term passive-DTS measurements and active-DTS measurements in order to highlight their respective potential to locate and quantify groundwater discharge into streams. On the one hand, passive-DTS measurements consist in monitoring natural temperature fluctuations to detect and localize groundwater inflows and characterize the temporal pattern of exchanges. Although easy to set up, the quantification of fluxes with this approach often remains difficult since it relies on energy balance models or on the coupling of distributed temperature measurements with additional punctual measurements. On the other hand, active-DTS methods, recently developed in hydrogeology, consist in continuously monitoring temperature changes induced by a heat source along a FO cable. Recent developments showed that this approach, although more complex to set up than passive-DTS measurements, can address the challenge of quantifying groundwater fluxes and their spatial distribution. Yet it has almost never been conducted in streambed sediments. In this study, both methods are combined by deploying FO cables in the streambed sediments of a first- and second-order stream within a small agricultural watershed. A numerical model is used to interpret passive-DTS measurements and highlight the temporal and spatial dynamic of groundwater discharge over the annual hydrological cycle. We underline the difficulties and the limitations of deploying a single FO cable to investigate groundwater discharge and show the impact of uncertainty on sediments thermal properties on the quantification of groundwater inflows. On the opposite, the active-DTS experiment allows estimating the spatial distribution of both the thermal conductivity and the groundwater flux at high resolution with very low uncertainties all along the heated section of FO cable. Our results highlight the added values of conducting active-DTS experiments, eventually combined with passive-DTS measurements, to fully investigate and characterize patterns of groundwater-stream water exchanges at the stream scale. The combination of both methods allows discussing the impact of topography and hydraulic conductivity variations on the variability of groundwater inflows in headwater catchments.