A review of microbial injury and recovery methods in food

Wu, Vivian C.H.

doi:10.1016/j.fm.2008.04.011

Cited by 336 publications

(206 citation statements)

References 82 publications

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“…it can be concluded that PEF processing is not an all or nothing event (Wu, 2008) and 394 that there was some evidence of SLI following the application of PEF. Interestingly, 395 there was no significant difference found between inactivation levels of E. coli 396 following treatment with PEF at 32kVcm -1 and pasteurisation (P>0.05).…”

Section: Effect Of Pef Processing On Microbial Viability 317mentioning

confidence: 93%

“…Microbial 84 inactivation due to PEF treatment is believed to be caused by disruption of the cell 85 membrane; a process known as 'electroporation' (Hamilton and Sale, 1967), which 86 results from recurring application of short pulses of high intensity electric fields 87 believed to be "killed" may only be sub-lethally injured. Microbial injury can be 98 defined as a microorganism that has suffered some form of stress but that has the 99 potential to regain viability and to form a colony under the right conditions (Wu, 2008). 100…”

Section: Introduction 50mentioning

confidence: 99%

“…It is believed that after treatments, by either thermal or non-thermal 106 technologies, there may be one population of microbes which are dead, another 107 population that are viable, and a third population that are sub-lethally injured (Wu, 108 2008). It is of the utmost importance to be able to distinguish between viable cells and 109 impaired cells in order to gain complete food safety (Wu, 2008). Escherichia coli (Walkling-Ribeiro et al, 2008).…”

Section: Introduction 50mentioning

confidence: 99%

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The effect of non-thermal processing technologies on microbial inactivation: An investigation into sub-lethal injury of Escherichia coli and Pseudomonas fluorescens

et al. 2014

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24In recent years, there has been an increased interest in food processing technologies that 25 could lessen the thermal impact on food products. In the present study, thermosonication 26 (TS) and pulsed electric fields (PEF), applied individually or in combination (TS/PEF), 27were investigated to determine their effects on inactivation and sub-lethal injury of 28Pseudomonas fluorescens and Escherichia coli. TS was applied at a low (L) and high 29 (H) wave amplitude (L; 18.6µm, H; 27.9µm, respectively), while PEF was applied at a 30 low and high electrical field strength (L; 29kVcm -1 , H; 32kVcm -1 , respectively). In 31 addition, the inhibitory effects of TS/PEF combined were assessed. For P. fluorescens, 32 when applied individually, TS and PEF resulted in ≤9% and ≤47% inactivation, 33 respectively, with 8% sub-lethal injury following PEF treatment. However, TS/PEF 34 treatment caused ≤48% inactivation and ≤34% sub-lethal injury, respectively. For E. 35 coli, TS caused ≤6% inactivation, and ≤2% sub-lethal injury, while PEF treatment alone 36 caused inactivation and sub-lethal injury of 86% and 29%, respectively. TS/PEF caused 37 a maximum of 66% inactivation, while sub-lethally injuring approximately 26% of the 38 of E. coli population. The present study confirms the ability of TS and PEF to inactivate 39 microorganisms, but shows that some bacteria were not killed, but sub-lethally injured. 40 41

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Section: Effect Of Pef Processing On Microbial Viability 317mentioning

confidence: 93%

Section: Introduction 50mentioning

confidence: 99%

Section: Introduction 50mentioning

confidence: 99%

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The effect of non-thermal processing technologies on microbial inactivation: An investigation into sub-lethal injury of Escherichia coli and Pseudomonas fluorescens

et al. 2014

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“…3A . The other is a novel model proposed here, in 1976;Hurst, 1984;Wu, 2008 . As a non-cultivation technique, the staining method, using two types of staining agents possessing different staining principles, have been applied to detect rapidly injured microbes e.g., Larsson et al, 2008 .…”

Section: Communicationmentioning

confidence: 99%

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

Tsuchido

2017

Biocontrol Sci.

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A novel double subculture method, termed DiVSaL Differential Viabilities between Solid and Liquid media method, for the enumeration of injured cell population of a microorganism, which occurs after some sublethal to lethal treatment, was proposed. In this method injured cells were enumerated as the differential value between viabilities determined with two different techniques, the conventional plate counting using a solid agar medium and the growth delay analysis using a liquid medium. In the former technique, the viable cell number is obtained as colony forming unit CFU formed on an agar medium where sublethally injured cells are as much rescued as possible. In the latter technique, on the other hand, the integrated viability defined by Takano and Tsuchido 1982 is introduced and is calculated from the growth delay of a stressed population, referred to unstressed one. For the growth delay analysis, in this paper, not only the original theoretical model, where the specific growth rate and therefore the defined G 10 value does not change after the exposure to a stress treatment, but also a novel modified theory, where the parameter changes, is proposed. On the theoretical background, this DiVSaL method as a double subculture method can be used to enumerate the injured cells without selection by addition of some inhibitor or by nutritional shortage.

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“…Em vista disto, a marcela tem sido citada em pesquisas de resgate etnográfico como ef etiv a planta medicinal, sendo as inflorescências utilizadas como infusos (chás) para efeitos antidiarreicos, antiespasmódicos, analgésico, antiinflamatória e antimicrobiana (Boscolo & Valle, 2008) e também conf irmadas a ativ idade farmacológica (Vendruscolo et al, 2005). Além disso, Zayachkivska et al (2005) relacionam estes efeitos ao conteúdo de flavonóides presentes em alta concentração na marcela, afirmando inclusive que a forma de extração destes princípios bioativos influenciam na atividade biológica.…”

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Atividade antibacteriana in vitro de inflorescências de Achyrocline satureioides (Lam.) DC. - Asteraceae ("macela", "marcela") sobre agentes bacterianos de interesse em alimentos

Mota

Carvalho

Wiest

2011

Rev. bras. plantas med.

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Dilution test in multiple tube system was used to determine the in vitro antibacterial activity in inflorescences of Achyrocline satureioides (Lam.) DC. -Asteraceae ("macela", "marcela"), expressed as intensity of bacterial inhibition activity (IINIB/bacteriostasis) and intensity of bacterial inactivation activity (IINAB/bactericidie), from ethanol (hydroalcoholic) and water (decoction) extraction forms on standardized inocula of Enterococcus faecalis (ATCC 19433), Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 11229) and Salmonella enteritidis (ATCC 11076). E. faecalis had the highest sensitivity, followed by S. aureus, while S. enteritidis and E. coli were more resistant. Of the extraction forms, the hydroalcoholic one showed intense and selective inhibition and/or inactivation capacity against four bacterial inocula. Decoctions were completely ineffective against the Gram-negative bacteria, whereas Gram-positive bacteria showed only bacteriostasis/inhibition.

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A review of microbial injury and recovery methods in food

Cited by 336 publications

References 82 publications

The effect of non-thermal processing technologies on microbial inactivation: An investigation into sub-lethal injury of Escherichia coli and Pseudomonas fluorescens

The effect of non-thermal processing technologies on microbial inactivation: An investigation into sub-lethal injury of Escherichia coli and Pseudomonas fluorescens

A Novel Double Subculture Method and Its Theory for the Enumeration of Injured Cells in Stressed Microbial Population

Atividade antibacteriana in vitro de inflorescências de Achyrocline satureioides (Lam.) DC. - Asteraceae ("macela", "marcela") sobre agentes bacterianos de interesse em alimentos

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