“…Microspheres can reduce side effects and immune rejection, produce efficiently, increase duration and stability of the structure, and are affordable, injectable, and sterilizable. 10 They are numerously used individually in bone reconstruction, 11 or in three-dimensional scaffolds 12 ; moreover, their ability to encapsulate drugs, genes, hormone, etc., increases their effectiveness. Despite the introduction of a variety of techniques for fabrication of these biocompatible vehicles such as spraying, 13 phase separation, 14 emulsion-based methods, 15,16 suspension or dispersion polymerization, 17 extrusion methods, 18 etc., single emulsion technique have been utilized copiously for the fabrication of gelatin microspheres or other carbohydrate proteins because of their dissolvability in aqueous medium.…”