Near infrared spectroscopy was used in discrimination of intact bovine teeth in terms of animal sex, diet, tooth type and place of origin. Discriminant Analysis (DA) models were developed and tested using a stratified random 70:30 data split to calibration and test sets. Of the discriminant techniques of Soft Independent Modelling of Class Analogy (SIMCA), Partial Least Squares – DA, Artificial Neural Networks (ANN) and Support Vector Machine (SVM), SVM and PLS-DA models performed best in most instances, with pretreatment choice impacting technique success. For test set prediction of animal sex, an accuracy of 95% was achieved using a PLS-DA model, and similar performance achieved using a SVM model. SVM, ANN and SIMCA models predicted three categories of tooth type (deciduous, permanent unerupted and erupted) with similar accuracies (89, 79 and 82%, respectively), however, permanent unerupted teeth were conflated with permanent erupted teeth. Prediction accuracy for ANN, SVM and PLS-DA models were also similar for discrimination of teeth from animals on a grain versus grass diet (PLS-DA 82%). There was considerable variation between models in prediction of geographic origin. Of the seven locations, two were discriminated with true positive rate around 70% using SVM and ANN models. Potential applications of this technology are discussed.