A review of non-coding RNA related to NF-κB signaling pathway in the pathogenesis of osteoarthritis

Feng, Tao; Wu, Qiaofeng

doi:10.1016/j.intimp.2022.108607

Cited by 18 publications

(17 citation statements)

References 73 publications

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“…In mammals, the NF-κB family consists of five distinct members (RelA (p65), RelB, C-Rel, NF κ B1 (P105/P50), and NF κ B2 (P100/P52)) ( Bonizzi and Karin, 2004 ). The expression of related genes triggered by non-coding RNA (NC RNAs) can widely activate the NF-κB signaling pathway and induce joint damage, leading to occurrence and development of OA ( Feng and Wu, 2022 ). There is increasing evidence that the NF-κB family plays a central role in the pro-inflammatory stress response of chondrocytes and controls their differentiation processes ( Marcu et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Zhuifeng tougu capsules inhibit the TLR4/MyD88/NF-κB signaling pathway and alleviate knee osteoarthritis: In vitro and in vivo experiments

et al. 2022

Front. Pharmacol.

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Background: Knee osteoarthritis (KOA), a chronic degenerative disease, is mainly characterized by destruction of articular cartilage and inflammatory reactions. At present, there is a lack of economical and effective clinical treatment. Zhuifeng Tougu (ZFTG) capsules have been clinically approved for treatment of OA as they relieve joint pain and inflammatory manifestations. However, the mechanism of ZFTG in KOA remains unknown.Purpose: This study aimed to investigate the effect of ZFTG on the TLR4/ MyD88/NF-κB signaling pathway and its therapeutic effect on rabbits with KOA. Study design:In vivo, we established a rabbit KOA model using the modified Videman method. In vitro, we treated chondrocytes with IL-1β to induce a proinflammatory phenotype and then intervened with different concentrations of ZFTG. Levels of IL-1β, IL-6, TNF-α, and IFN-γ were assessed with histological observations and ELISA data. The effect of ZFTG on the viability of chondrocytes was detected using a Cell Counting Kit-8 and flow cytometry. The protein and mRNA expressions of TLR2, TLR4, MyD88, and NF-κB were detected using Western blot and RT-qPCR and immunofluorescence observation of NF-κB p65 protein expression, respectively, to investigate the mechanism of ZFTG in inhibiting inflammatory injury of rabbit articular chondrocytes and alleviating cartilage degeneration.Results: The TLR4/MyD88/NF-κB signaling pathway in rabbits with KOA was inhibited, and the levels of IL-1β, IL-6, TNF-α, and IFN-γ in blood and cell were significantly downregulated, consistent with histological results. Both the protein and mRNA expressions of TLR2, TLR4, MyD88, NF-κB, and NF-κB p65 proteins in that nucleus decreased in the ZFTG groups. Moreover, ZFTG

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Section: Discussionmentioning

confidence: 99%

Zhuifeng tougu capsules inhibit the TLR4/MyD88/NF-κB signaling pathway and alleviate knee osteoarthritis: In vitro and in vivo experiments

et al. 2022

Front. Pharmacol.

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“…Cartilage inflammation caused by the NF-κB signaling pathway is the main factor aggravating the pathology of OA ( Feng and Wu, 2022 ). Under normal circumstances, p65 binds to its inhibitory protein IκBα and inactivates the NF-κB pathway.…”

Section: Discussionmentioning

confidence: 99%

Ginkgolide C slows the progression of osteoarthritis by activating Nrf2/HO-1 and blocking the NF-κB pathway

Jia

Zhao³

et al. 2022

Front. Pharmacol.

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Osteoarthritis (OA) is driven by chronic low-grade inflammation and subsequent cartilage degradation. OA is the most prevalent degenerative joint disease worldwide, and its treatment remains a challenge. The aim of this study was to explore the potential effects and mechanism underlying the anti-OA properties of ginkgolide C (GC). Protective effects of GC on hydrogen peroxide (H2O2)-treated rat chondrocytes were evaluated using ELISA, qPCR, western blot analysis, flow cytometry, ROS detection and immunofluorescence in vitro. Ameliorating effects of GC on cartilage degeneration in rats were evaluated through behavioral assays, microcomputed tomography, histopathological analysis, western blot analysis and ELISA in vivo. In vitro, GC treatment inhibited the release of pro-apoptotic factors induced by H2O2 and promoted the release of the anti-apoptotic proteins. In addition, GC decreased the expression of matrix metalloproteinase (MMP3 and MMP13), thrombospondin motifs 4 (ADAMTS4), and inflammatory mediators inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and SOX9 thereby inhibiting extracellular matrix (ECM) degradation. Mechanistically, GC exerts its anti-apoptotic and anti-inflammatory effects by upregulating the oxidative stress signaling Nrf2/HO-1 pathway and preventing p65 from binding to DNA. Similarly, In a rat model with post-traumatic OA (PTOA) induced by anterior cruciate ligament transection (ACLT), GC inhibited joint pain, cartilage destruction, and abnormal bone remodeling of subchondral bone. GC inhibited H2O2-induced chondrocyte apoptosis through Nrf2/HO-1 and NF-κB axis, exerted anti-inflammatory effects, and inhibited cartilage degeneration in rat OA. Our findings advanced the concept that GC may contribute to cartilage metabolism through anti-inflammatory and anti-apoptotic effects, and the identified GC is a potential therapeutic agent for the treatment of OA.

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“…Non-coding RNA (ncRNA) is commonly employed by RNA that does not encode a protein. These ncRNAs include microRNA, snoRNAs, other small regulatory RNAs, and lncRNAs [ 10 , 11 , 12 ]. The latter is a class of ncRNAs typically longer than 200 nucleotides.…”

Section: Long Non-coding Rnas and Potential Therapeutic Targeting In ...mentioning

confidence: 99%

“…LncRNAs are mainly transcribed by RNA polymerase II, typically by a 5′7-methylguanosine cap and a 3′ poly (A) tail. Similar to messenger RNAs, they are involved in the pathogenesis of cancer [ 11 , 13 , 14 , 15 , 16 , 17 , 18 ]. Different classes of lncRNAs are generated from introns, exons, intergenic regions, telomerases, enhancers, or promoters [ 19 ].…”

Section: Long Non-coding Rnas and Potential Therapeutic Targeting In ...mentioning

confidence: 99%

The Regulatory Functions and the Mechanisms of Long Non-Coding RNAs in Cervical Cancer

Yang

Al-Hendy

2022

Cells

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Cervical cancer is one of the leading causes of death in gynecology cancer worldwide. High-risk human papillomaviruses (HPVs) are the major etiological agents for cervical cancer. Still, other factors also contribute to cervical cancer development because these cancers commonly arise decades after initial exposure to HPV. So far, the molecular mechanisms underlying the pathogenesis of cervical cancer are still quite limited, and a knowledge gap needs to be filled to help develop novel strategies that will ultimately facilitate the development of therapies and improve cervical cancer patient outcomes. Long non-coding RNAs (lncRNAs) have been increasingly shown to be involved in gene regulation, and the relevant role of lncRNAs in cervical cancer has recently been investigated. In this review, we summarize the recent progress of ascertaining the biological functions of lncRNAs in cervical cancer, from the perspective of cervical cancer proliferation, invasion, and metastasis. In addition, we provide the current state of knowledge by discussing the molecular mechanisms underlying the regulation and emerging role of lncRNAs in the pathogenesis of cervical cancer. Comprehensive and deeper insights into lncRNA-mediated alterations and interactions in cellular events will help develop novel strategies to treat patients with cervical cancer.

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A review of non-coding RNA related to NF-κB signaling pathway in the pathogenesis of osteoarthritis

Cited by 18 publications

References 73 publications

Zhuifeng tougu capsules inhibit the TLR4/MyD88/NF-κB signaling pathway and alleviate knee osteoarthritis: In vitro and in vivo experiments

Zhuifeng tougu capsules inhibit the TLR4/MyD88/NF-κB signaling pathway and alleviate knee osteoarthritis: In vitro and in vivo experiments

Ginkgolide C slows the progression of osteoarthritis by activating Nrf2/HO-1 and blocking the NF-κB pathway

The Regulatory Functions and the Mechanisms of Long Non-Coding RNAs in Cervical Cancer

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