A Review of Problems and Progress in Research on Viruses and Virus Diseases of Narcissus in Britain

Brunt, A.A.

doi:10.17660/actahortic.1980.110.1

Cited by 14 publications

(5 citation statements)

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“…Unpublished data by Brunt [ 14 ] that "some isolates [of NMV] are associated with flower mottling in cultivars such as Chanter and Spellbinder (A.A. Brunt and S. Phillips, unpublished information )" support our findings that NMV infection is closely linked to colour break in reverse bicolours as both of these cultivars are of that type (J.A. Hunter personal communication ).…”

Section: Discussionsupporting

confidence: 82%

Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Hunter

Fletcher

Davies

et al. 2011

Virol J

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BackgroundDaffodils (Narcissus pseudonarcissus) are one of the world's most popular ornamentals. They also provide a scientific model for studying the carotenoid pigments responsible for their yellow and orange flower colours. In reverse bicolour daffodils, the yellow flower trumpet fades to white with age. The flowers of this type of daffodil are particularly prone to colour break whereby, upon opening, the yellow colour of the perianth is observed to be 'broken' into patches of white. This colour break symptom is characteristic of potyviral infections in other ornamentals such as tulips whose colour break is due to alterations in the presence of anthocyanins. However, reverse bicolour flowers displaying colour break show no other virus-like symptoms such as leaf mottling or plant stunting, leading some to argue that the carotenoid-based colour breaking in reverse bicolour flowers may not be caused by virus infection.ResultsAlthough potyviruses have been reported to cause colour break in other flower species, enzyme-linked-immunoassays with an antibody specific to the potyviral family showed that potyviruses were not responsible for the occurrence of colour break in reverse bicolour daffodils. Colour break in this type of daffodil was clearly associated with the presence of large quantities of rod-shaped viral particles of lengths 502-580 nm in tepals. Sap from flowers displaying colour break caused red necrotic lesions on Gomphrena globosa, suggesting the presence of potexvirus. Red necrotic lesions were not observed in this indicator plant when sap from reverse bicolour flowers not showing colour break was used. The reverse transcriptase polymerase reactions using degenerate primers to carla-, potex- and poty-viruses linked viral RNA with colour break and sequencing of the amplified products indicated that the potexvirus Narcissisus mosaic virus was the predominant virus associated with the occurrence of the colour break.ConclusionsHigh viral counts were associated with the reverse bicolour daffodil flowers that were displaying colour break but otherwise showed no other symptoms of infection. Narcissus mosaic virus was the virus that was clearly linked to the carotenoid-based colour break.

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Section: Discussionsupporting

confidence: 82%

Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Hunter

Fletcher

Davies

et al. 2011

Virol J

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“…3), an aetiology which could otherwise be questioned because of the poor correlation between NLSYV infection and the disease (Text- fig. The name, late season yellows, was first introduced by Brunt (1980) but no detailed description of the disease has been published. Indeed, because NLSYV infected plants remain symptomless for the greater part of the growing season, and may not express symptoms at all in some seasons, the incidence of symptoms may greatly underestimate the incidence of infection.…”

Section: Discussionmentioning

confidence: 99%

“…With the advent of virus-tested narcissus schemes in the UK (Brunt, 1980, Mowat, Dixon & Fordyce, 1986) the need for reliable means of virus identification and detection is vital if the schemes are to be managed successfully. Of the diseases associated with potyvirus infection in narcissus, there is little doubt that late season yellows is the most widespread in commercial sotcks grown in the UK, apparently totally affecting all plants of some, if not all, stocks of several important cultivars.…”

Section: Introductionmentioning

confidence: 99%

Narcissus late season yellows potyvirus: symptoms, properties and serological detection

Mowat

Duncan

Dawson

1988

Annals of Applied Biology

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S U M M A R YNarcissus late season yellows virus (NLSYV) was obtained, apparently unmixed with other viruses, in selected naturally infected narcissus plants. The virus was not transmitted by manual inoculation to any of the 12 herbaceous species tested but was transmitted by Myzuspersicae to virus-tested narcissus plants, which then developed symptoms characteristic of late season yellows. Typically, symptoms develop late in the growing season but their expression is unreliable. NLSYV particles, which measured c. 750 x 12 nm, were purified from fresh leaf extracts of narcissus in 0-3 M sodium citrate containing 10 mM sodium EDTA and 10 mM sodium DIECA by differential centrifugation followed by clarification with diethyl ether, gel filtration and isopycnic banding in caesium sulphate. Using antibodies from antisera prepared to virus particles, NLSYV was detected by ELISA in 14 of the 18 cultivars examined and nine seemed totally infected. Detection of the virus by immunosorbent electron microscopy was as sensitive as by ELISA but neither test detected all infections. The virus was detected by ELISA only in samples collected after flowering and capsid antigen concentration was greatest in the distal region of leaves. The reliability of detection depended on the cultivar. Immuno-electron microscopy tests confirmed the Occurrence of a distinct potyvirus, presumably narcissus degeneration, in narcissus cv. Grand Soleil d'Or, failed to detect any virus other than NLSYV in narcissus plants affected by white streak disease, and showed that NLSYV is related to three of five other potyviruses tested.

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“…Narcissus degeneration virus (NDV), narcissus late season yellows virus (NLSYV) and narcissus yellow stripe virus (NYSV), belonging to the genus Potyvirus of the family Potyviridae , are the most frequently seen viruses infecting narcissus [ 3 , 5 , 6 , 7 ]. NDV and NLSYV were first reported in plants of N. tazetta and N. pseudonarcissus by Brunt et al [ 8 ]. NYSV infection in plants of N. spp.…”

Section: Introductionmentioning

confidence: 99%

Synonymous Codon Usage Analysis of Three Narcissus Potyviruses

Ding

Guo³

et al. 2022

Viruses

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Narcissus degeneration virus (NDV), narcissus late season yellows virus (NLSYV) and narcissus yellow stripe virus (NYSV), which belong to the genus Potyvirus of the family Potyviridae, cause significant losses in the ornamental value and quality of narcissus. Several previous studies have explored the genetic diversity and evolution rate of narcissus viruses, but the analysis of the synonymous codons of the narcissus viruses is still unclear. Herein, the coat protein (CP) of three viruses is used to analyze the viruses’ phylogeny and codon usage pattern. Phylogenetic analysis showed that NYSV, NDV and NLSYV isolates were divided into five, three and five clusters, respectively, and these clusters seemed to reflect the geographic distribution. The effective number of codon (ENC) values indicated a weak codon usage bias in the CP coding region of the three narcissus viruses. ENC-plot and neutrality analysis showed that the codon usage bias of the three narcissus viruses is all mainly influenced by natural selection compared with the mutation pressure. The three narcissus viruses shared the same best optimal codon (CCA) and the synonymous codon prefers to use codons ending with A/U, compared to C/G. Our study shows the codon analysis of different viruses on the same host for the first time, which indicates the importance of the evolutionary-based design to control these viruses.

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A Review of Problems and Progress in Research on Viruses and Virus Diseases of Narcissus in Britain

Cited by 14 publications

References 0 publications

Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Narcissus late season yellows potyvirus: symptoms, properties and serological detection

Synonymous Codon Usage Analysis of Three Narcissus Potyviruses

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