A review of proficiency exercises offered by the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) and Moffett Proficiency Testing Laboratory from 2012 to 2018

Nemser, Sarah M.; Lindemann, Samantha; Chen, Yang; López, Salvador Moreno; Pickens, Shannon; Ulaszek, Jodie; Kmet, Matthew; Powers, Christopher M.; Ensley, Steve; Schrunk, Dwayne E.; Rumbeiha, Wilson K.; Tkachenko, Andriy; Guag, Jake; Cerić, Olgica; Jones, Jennifer L.; Reimschuessel, Renate; Reddy, Ravinder

doi:10.1007/s00769-021-01471-x

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…These evaluations act as benchmarks, assessing the efficacy of current protocols and identifying areas that require refinement and adjustment. Insights gained from such assessments not only enable laboratories to proactively address potential shortcomings, but also foster a culture of continuous improvement, where the pursuit of analytical excellence in vitamin analysis becomes a sustained commitment rather than a fleeting goal [115][116][117].…”

Section: Navigating the Challenges Of Consistency And Accuracymentioning

confidence: 99%

Insights into Analytical Precision: Understanding the Factors Influencing Accurate Vitamin A Determination in Various Samples

Shastak,

Pelletier,

Kuntz

2024

Analytica

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The accurate assessment of vitamin A in animal feed and tissues is vital for safeguarding animal well-being and ensuring high-quality nutritional feed. However, challenges in achieving precise results persist, necessitating a comprehensive understanding of the influencing factors. This review delves into the historical progression of analytical techniques, from colorimetric assays and spectrophotometry to advanced chromatographic methods and non-destructive spectroscopic approaches. Factors influencing analytical precision are scrutinized, encompassing sample preparation, storage conditions, interfering substances, and human errors. The crucial role of quality control and standardized protocols in ensuring the reproducibility and reliability of results is emphasized. Moreover, this review highlights the need for tailored analytical approaches to account for the complexities of sample matrices and the significance of cutting-edge technologies, including on-site analysis and data science integration, in enhancing analytical precision. By acknowledging the challenges and prospects in vitamin A analysis, this paper provides insights for optimizing analytical methodologies and promoting animal welfare and product safety.

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Section: Navigating the Challenges Of Consistency And Accuracymentioning

confidence: 99%

Insights into Analytical Precision: Understanding the Factors Influencing Accurate Vitamin A Determination in Various Samples

Shastak,

Pelletier,

Kuntz

2024

Analytica

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“…The ICE was designed to provide a safe and structured way of evaluating the utility of potential methods without grading the performance of participants. 21 The PT was designed to evaluate the performance of participants. 12 For both ICE and PT, the procedures for preparing samples, shipping samples, submitting results, and homogeneity and stability analyses all followed the International Organization for Standardization ISO/IEC 17043:2010 standard.…”

mentioning

confidence: 99%

Validation and interlaboratory comparison of anticoagulant rodenticide analysis in animal livers using ultra-performance liquid chromatography–mass spectrometry

Chen,

Lopez,

Reddy

et al. 2023

J VET Diagn Invest

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Anticoagulant rodenticides (ARs) are used to control rodent populations. Poisoning of non-target species can occur by accidental consumption of commercial formulations used for rodent control. A robust method for determining ARs in animal tissues is important for animal postmortem diagnostic and forensic purposes. We evaluated an ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) method to quantify 8 ARs (brodifacoum, bromadiolone, chlorophacinone, coumachlor, dicoumarol, difethialone, diphacinone, warfarin) in a wide range of animal (bovine, canine, chicken, equine, porcine) liver samples, including incurred samples. We further evaluated UPLC-MS in 2 interlaboratory comparison (ILC) studies; one an ILC exercise (ICE), the other a proficiency test (PT). The limits of detection of UPLC-MS were 0.3–3.1 ng/g, and the limits of quantification were 0.8–9.4 ng/g. The recoveries obtained using UPLC-MS were 90–115%, and relative SDs were 1.2–13% for each of the 8 ARs for the 50, 500, and 2,000 ng/g spiked liver samples. The overall accuracy from the laboratories participating in the 2 ILC studies (4 and 11 laboratories for ICE and PT studies, respectively) were 86–118%, with relative repeatability SDs of 3.7–11%, relative reproducibility SDs of 7.8–31.2%, and Horwitz ratio values of 0.5–1.5. Via the ILC studies, we verified the accuracy of UPLC-MS for AR analysis in liver matrices and demonstrated that ILC can be utilized to evaluate performance characteristics of analytical methods.

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Proficiency test of SARS-CoV-2 Omicron variant detection in diagnostics samples by veterinary diagnostic laboratories

Singh,

Miller,

Nemser

et al. 2024

Accred Qual Assur

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Veterinary diagnostic laboratories (VDLs) play a critical role in screening both human and animal samples for SARS-CoV-2. To evaluate the SARS-CoV-2 detection methods used by VDLs, a proficiency test was performed by the US Food and Drug Administration’s Veterinary Laboratory and Investigation and Response Network in collaboration with two other organizations. Thirty-two sets of 12 blind-coded samples were prepared by fortifying Molecular Transport Medium (MTM) or feline feces with SARS-CoV-2 Omicron variant or non-SARS-CoV-2 equine coronavirus RNA at various concentrations and shipped to 32 participants for blinded (unbiased) analysis. Results were analyzed according to the principles of International Organization for Standardization 16140-2:2016 using two approaches such as establishing the rate of detection (ROD) and the success rate by applying the analysis of binary outcome by logit approach. ROD provided the overall assessment of laboratories performance, whereas the novel logit approach provided an insight to more specific analysis based on the complexity of each sample. The ROD was 83% and 98% for MTM samples at 200 and 20000 genome copies per 100 µL, respectively. Fecal samples were classified as challenging exploratory, and results were not included in the assessment of performance but discussion purposes only. Fecal samples exhibited matrix interference impacting the performance. The ROD was 44% and 89% for fecal samples at 2000 and 20000 genome copies per 100 µL, respectively. The non-COVID coronavirus RNA, which was used to address the specificity, did not interfere with methods used. Establishing the success rate by evaluating the qualitative results (detected/not detected) applying a logit approach revealed that, out of thirty-two participants, twenty-eight had satisfactory results, one participant had unsatisfactory results, and three participants had questionable results for MTM samples. For fecal samples, three participants out of thirty-two did not meet the expectations at higher concentrations. Lower concentrations of fecal samples were excluded from this analysis. Again, the fecal samples were considered as challenge samples and the results were provided to assist participants in their continuous efforts to improve their performance and not to evaluate their performance.

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A review of proficiency exercises offered by the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) and Moffett Proficiency Testing Laboratory from 2012 to 2018

Cited by 3 publications

References 30 publications

Insights into Analytical Precision: Understanding the Factors Influencing Accurate Vitamin A Determination in Various Samples

Insights into Analytical Precision: Understanding the Factors Influencing Accurate Vitamin A Determination in Various Samples

Validation and interlaboratory comparison of anticoagulant rodenticide analysis in animal livers using ultra-performance liquid chromatography–mass spectrometry

Proficiency test of SARS-CoV-2 Omicron variant detection in diagnostics samples by veterinary diagnostic laboratories

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