A review of random amplified polymorphic DNA (RAPD) markers in fish research

Ali, Bahy A.; Huang, Tian-Hua; Qin, Danian; Wang, Xiaomei

doi:10.1007/s11160-005-0815-0

Cited by 57 publications

(34 citation statements)

References 87 publications

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“…But the band-sharing using the different indices such as similarity and heterozygosity are sufficient to distinct some of the sampling locality in the Garhwal and Kumaun region. But within population it is difficult to detect the level of genetic diversity using the RAPD marker in our study and similar in other study which suggested the use of mitochondrial markers (Ali et al, 2004;Cadena et al, 2011). The UPGMA analysis permitted the clustering of different individuals of three Schistura species from Garhwal and Kumaun region with distinguished clade at population level but unable to distinguished individuals of the same population and similar finding were also reported by (Rodrigues et al, 2007).…”

Section: Discussionsupporting

confidence: 82%

Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker

Negi¹,

Joshi²

2015

Asian J. of Biotechnology

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Genetic based study provide emphasis on the population study of geographically isolated population, gene flow among them and evolutionary history of the species. In the present study, we aimed to detect the genetic variations in species of genus Schistura from Uttarakhand. Very little is known for the pattern of distribution and genetic diversity of the species of genus Schistura from the Garhwal and Kumaun region. Out of six primer only four primers were used to generate the fragments patterns from the samples collected. Polymorphism within and between the populations were assayed and total 60 bands were amplified ranging from 1-150 kb. Maximum number of bands was observed in Schistura montanus from the Srinagar, Garhwal and Uttarkashi regions. Total 60 bands were amplified in the all four primer with high percentage of polymorphic loci 50% Schistura montanus from Garhwal region and 28.8% in the S. montanus from the Kumaun region were observed. The level of heterozygosity were found 0.006-0.18 in the all three species. The percentage of polymorphism was 50% within the populations and high heterozygosity the sample of S. montanus when compared with other species. Observed pattern of RAPD markers reveals that the samples from the Garhwal region exhibit high diversity and used four primers are sufficient to distinguish the different population in the both region except the samples of the S. gangiticus of Garhwal region.

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Section: Discussionsupporting

confidence: 82%

Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker

Negi¹,

Joshi²

2015

Asian J. of Biotechnology

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“…Ultimamente, a técnica de RAPD ("Random Amplified Polymorphic DNA") tem sido um eficiente instrumento utilizado em estudos de diversidade genética de peixes (Liu & Cordes, 2004), em razão do seu alto potencial de detecção de polimorfismo (Ali et al, 2004) e por se basear na PCR ("Polymerase Chain Reaction"), que é uma ferramenta simples, rápida e eficiente (Ferreira & Grattapaglia, 1998).…”

Section: Introductionunclassified

Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Jacometo

Barrero

Rodríguez-Rodríguez

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a diversidade genética em quatro estoques de tambaquis (Colossoma macropomum) de diferentes regiões do Brasil, por meio de marcador RAPD. Foram utilizados 10 iniciadores para analisar 116 indivíduos, coletados de pisciculturas nos municípios de Urupá, RO, Teixeirópolis, RO, Neópolis, SE e Sorriso, MT. Foram encontradas diferenças nas frequências de 67 fragmentos, com um fragmento exclusivo em Sorriso e dois em Neópolis. Observaram-se altos valores de polimorfismo (72,92 a 83,33%), diversidade genética de Nei (0,27 a 0,30) e índice de Shannon (0,39 a 0,45). A análise da variância molecular, demonstrou que a maior parte da variação está dentro de cada estoque e não entre os estoques.A identidade e a distância genética entre os agrupamentos variou de 0,93 a 0,98 e 0,02 a 0,07, respectivamente, com menos distância entre os agrupamentos Urupá x Sorriso e entre Teixerópolis x Neópolis. A diferenciação genética variou de baixa a moderada (F st = 0,03 a 0,15) e o número de migrantes por geração foi alto (Nm = 5,96 a 24,3), entre os agrupamentos. Os estoques apresentam alta variabilidade e baixa diferenciação e distância genética entre si.Termos para indexação: Colossoma macropomum, diversidade genética, RAPD. Genetic variability of tambaqui (Teleostei: Characidae) from different regions of BrazilAbstract -The objective of this study was to evaluate the genetic diversity of four broodstocks of tambaqui (Colossoma macropomum) from different regions of Brazil using RAPD markers. Ten primers were used to analyze 116 individuals, collected from fish cultures of three municipalities in Brazil: Urupá, RO; Teixeirópolis, RO; Neópolis, SE; and Sorriso, MT. Differences in the frequencies of 67 fragments were found, with a unique fragment in Sorriso and two in Neópolis. High values of polymorphism (72.92 to 83.33%), Nei's genetic diversity (from 0.27 to 0.30) and Shannon index (from 0.39 to 0.45) were observed. Analysis of molecular variance showed that most of the variation is within each broodstock and not among them. The identity and genetic distance among the groups ranged from 0.93 to 0.98 and from 0.02 to 0.07, respectively, with less distance between clusters Urupá x Sorriso and Teixerópolis x Neópolis. Genetic differentiation ranged from low to moderate (F st = 0.03 to 0.15) and the number of migrants per generation was high (Nm = 5.96 to 24.3) among the groups. Stocks have high variability and low genetic differentiation and distance among them.

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“…The RAPD method is used in fish studies aimed at the genetic monitoring of population stocks (Wasko et al, 2004), the identification of species and broodstocks (Partis and Wells, 1996;Prioli et al, 2002;Ali et al, 2004), structuring analysis of natural populations (Wasko and Galetti Jr., 2002;Almeida et al, 2003;Hatanaka and Galetti Jr., 2003;Leuzzi et al, 2004;Matoso et al, 2004;Sofia et al, 2006) and genetic variability in populations or species not yet studied from the genetic standpoint (Ramella et al, 2006). In other groups, RAPD Genetic variability in Steindachneridion melanodermatum markers have been combined with methodologies of a less arbitrary nature in order to generate better defined DNA patterns, i.e., DNA fingerprinting, for the purpose of molecular characterization (Aggarwal et al, 2008;Demir et al, 2010), for analysis of genetic diversity (Brahmane et al, 2008;Rabello et al, 2008) to provide practical information for selection of parentals in planning breeding strategies (Ahmad et al, 2010), and to determine phylogenetic relationships (Marouelli et al, 2010) besides population differentiation (Klinbunga et al, 2010) between wild and cultivated stocks (Sesli and Yegenoglu, 2010).…”

Section: Discussionmentioning

confidence: 99%

Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation

Matoso¹,

Silva²,

Cortinhas³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F 1 fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens. The results suggest different genetic lineages in sympatry in nature. Heterozygosity and percentage of polymorphic loci were 0.31 and 79% and 0.23 and 62%, respectively, for the two populations of wild specimens and 0.26 and 66%, respectively, for those born in captivity.

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A review of random amplified polymorphic DNA (RAPD) markers in fish research

Cited by 57 publications

References 87 publications

Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker

Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker

Variabilidade genética em tambaquis (Teleostei: Characidae) de diferentes regiões do Brasil

Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation

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