The purpose of this investigation is twofold: 1. to determine whether the nitrogen in the vegetative parts of the corn plant at pollination is adequate to supply the maturing grain, if not, the contribution of soil nitrogen to the overall nitrogen supply after pollination; 2. to determine to what extent certain nitrogen compounds in the stalks and leaves at pollination contribute to the nitrogen of the maturing grain. The plan was to study corn grown under field conditions on a naturally fertile soil in which there was no deficiency in the nutrient-supplying capacity for the crop. It was also hoped this investigation would disclose new facts about the nitrogen metabolism of the corn plant which might have a bearing on practical aspects of corn protein problems.There have been many investigations concerned with various phases of nitrogen metabolism of plants. The bulk of the recent work has dealt with the effect of changing environmental and nitrogen nutritional conditions on the composition of the plant (30,31,32,33,34,35). In view of the many excellent reviews on the subject (5, 17, 20, 27, 28) no attempt will be made to review the literature here. Methods
PLANTING AND SAMPLINGTo accomplish the aims set forth in the introduction, two corn hybrids, U.S. 13 and Funk's G-80, were planted in a rich dark-colored soil classified as Drummer clay loam. No commercial fertilizer was applied for this crop, although a good crop of second-year mammoth clover was plowed under.The hybrids were planted June 1, 1945, and grown at the rate of three plants per hill. To provide for the sampling technique, each hybrid plot was divided into 10 blocks. Only the even-numbered hills were used for samples. At sampling, one from each block was chosen at random. The plants were examined and those showing too much deviation from the average stage of development were discarded. The remaining plants were used as a composite sample, which consisted of the above-ground parts of 14 to 26 plants.