The synthesis of high-quality chirality-pure single-walled carbon nanotubes (SWCNTs) is vital for their applications. It is of high importance to modernize the synthesis processes to decrease the synthesis temperature and improve the quality and yield of SWCNTs. This review is dedicated to the chirality-selective synthesis, sorting of SWCNTs, and applications of chirality-pure SWCNTs. The review begins with a description of growth mechanisms of carbon nanotubes. Then, we discuss the synthesis methods of semiconducting and metallic conductivity-type and single-chirality SWCNTs, such as the epitaxial growth method of SWCNT (“cloning”) using nanocarbon seeds, the growth method using nanocarbon segments obtained by organic synthesis, and the catalyst-mediated chemical vapor deposition synthesis. Then, we discuss the separation methods of SWCNTs by conductivity type, such as electrophoresis (dielectrophoresis), density gradient ultracentrifugation (DGC), low-speed DGC, ultrahigh DGC, chromatography, two-phase separation, selective solubilization, and selective reaction methods and techniques for single-chirality separation of SWCNTs, including density gradient centrifugation, two-phase separation, and chromatography methods. Finally, the applications of separated SWCNTs, such as field-effect transistors (FETs), sensors, light emitters and photodetectors, transparent electrodes, photovoltaics (solar cells), batteries, bioimaging, and other applications, are presented.