A review of synthetic biology tools in Yarrowia lipolytica

Cao, Linshan; Li, Jiajie; Yang, Zihan; Hu, Xiao; Wang, Pengchao

doi:10.1007/s11274-023-03557-9

Cited by 7 publications

(2 citation statements)

References 138 publications

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“…With the development of synthetic biology, a variety of promoters were characterized in model yeast S . cerevisiae [ [21] , [22] , [23] ] or other non-conventional yeast such as Yarrowia lipolytica [ 24 , 25 ], Pichia pastoris [ 26 , 27 ]. However, there are limited promoters other than methanol inducible promoters that have been characterized in O. polymorph [ 28 ], which is incompatible for pathway optimization under non-methanol medium.…”

Section: Introductionmentioning

confidence: 99%

Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha

Ye,

Gao,

et al. 2024

Synthetic and Systems Biotechnology

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Section: Introductionmentioning

confidence: 99%

Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha

Ye,

Gao,

et al. 2024

Synthetic and Systems Biotechnology

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“…Y. lipolytica demonstrates exceptional growth potential in high-density industrial fermenter environments (4), rendering it highly suitable for various industrial applications. Moreover, the development of genetic tools specific to Y. lipolytica has experienced significant progress in recent years (5), although not reaching the same level of advancement as its relatively closely related counterpart, Saccharomyces cerevisiae . The status of Y. lipolytica as an industrial workhorse is further reinforced by its Generally Recognized as Safe (GRAS) designation by the American Food and Drug Administration (FDA) (GRN No.…”

Section: Introductionmentioning

confidence: 99%

Impact of central carbon metabolism bypasses on the production of beta-carotene inYarrowia lipolytica

Markuš,

Soldat,

Magdevska

et al. 2023

Preprint

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Yarrowia lipolyticais an oleaginous yeast with ever growing popularity in the metabolic engineering circles. It is well known for its ability to accommodate a high carbon flux through acetyl-CoA and is being extensively studied for production of chemicals derived from it.We investigated the effects of modifying the upstream metabolism leading to acetyl-CoA on beta-carotene production, including its titer, yield, and content. We examined the pyruvate and the phosphoketolase bypass, both of which are stoichiometrically favorable for the production of acetyl-CoA and beta-carotene. Additionally, we examined a set of genes involved in the carnitine shuttle. We constructed a set of parental strains derived from theY. lipolyticaYB-392 wild-type strain, each with a different capacity for beta-carotene production, and introduced genes for the metabolic bypasses in each of the constructed parental strains. Subsequently, we subjected these constructed strains to a series of fermentation experiments.We discovered that altering the upstream metabolism in most cases led to a decrease in performance for production of beta-carotene. Most notably, a set of genes used for the pyruvate bypass (YlPDC2,YlALD5, andYlACS1) and the phosphoketolase bypass (LmXPKandCkPTA) resulted in the reduction of more than 30%.Our findings contribute to our understanding ofY. lipolytica’s metabolic capacity and suggest that production of beta-carotene is most likely not limited solely by the acetyl-CoA supply. We also highlight a complex nature of engineeringY. lipolytica, as most of the results from studies using a different strain background did not align with our findings.

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Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies

Li,

Ni,

et al. 2024

World J Microbiol Biotechnol

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A review of synthetic biology tools in Yarrowia lipolytica

Cited by 7 publications

References 138 publications

Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha

Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha

Impact of central carbon metabolism bypasses on the production of beta-carotene inYarrowia lipolytica

Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies

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