A review of the practical application of micro-aeration and oxygenation for hydrogen sulfide management in anaerobic digesters

Muller, Christopher; Guevarra, Karla; Summers, Amanda; Pierce, Laurie; Shahbaz, Parisa; Zemke, Peter E.; Woodland, Karina; Hollingsworth, Vicky; Nakhla, George; Bell, Katherine Y.; Bronstad, Embrey

doi:10.1016/j.psep.2022.07.009

Cited by 17 publications

(9 citation statements)

References 66 publications

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“…63 Selenocysteine could also be beneficial for microaeration strategies to enhance hydrolysis and methane production. 41,42 In this enrichment, the increased relative abundance of Methanobacterium and Methanobrevibacter corresponded to an escalating selenocysteine ratio, indicating that selenocysteine incorporation confers a competitive advantage (Figure 4). Interestingly, the oxygen-sensitive class I methanogens (Methanobacterium and Methanobrevibacter) dominated the H-ORP environments, while the L-ORP environment had increased abundance of the more oxygen-tolerant class II methanogens (Methanosarcina and Methanoculleus).…”

Section: ■ Results and Discussionmentioning

confidence: 95%

“…Selenocysteine incorporation could be beneficial in anaerobic digestion processes, in which operational conditions often lead to inadvertent exposure to oxygen, which impacts efficiency . Selenocysteine could also be beneficial for microaeration strategies to enhance hydrolysis and methane production. , …”

Section: Resultsmentioning

confidence: 99%

“…Deciphering the response of methanogens to varying redox environments and the role of selenocysteine will aid in advancing our understanding of biogeochemical cycles and enhance the design of more robust and efficient digestion and biogas production processes. , Therefore, this study presents a proteomic analysis of methanogenic cultures cultivated in the presence of the reducing agent 9,10-anthraquinone-2,7-disulfonate disodium (AQDS) and without AQDS. While the oxidation–reduction potential (ORP) was monitored with probes, proteomic analysis was utilized to examine the oxidative stress response and discern selenium-associated changes in the proteome.…”

Section: Introductionmentioning

confidence: 99%

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The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Kleikamp,

Palacios,

Kofoed

et al. 2024

Environ. Sci. Technol.

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Methanogenesis is a critical process in the carbon cycle that is applied industrially in anaerobic digestion and biogas production. While naturally occurring in diverse environments, methanogenesis requires anaerobic and reduced conditions, although varying degrees of oxygen tolerance have been described. Microaeration is suggested as the next step to increase methane production and improve hydrolysis in digestion processes; therefore, a deeper understanding of the methanogenic response to oxygen stress is needed. To explore the drivers of oxygen tolerance in methanogenesis, two parallel enrichments were performed under the addition of H2/CO2 in an environment without reducing agents and in a redox-buffered environment by adding redox mediator 9,10-anthraquinone-2,7-disulfonate disodium. The cellular response to oxidative conditions is mapped using proteomic analysis. The resulting community showed remarkable tolerance to high-redox environments and was unperturbed in its methane production. Next to the expression of pathways to mitigate reactive oxygen species, the higher redox potential environment showed an increased presence of selenocysteine and selenium-associated pathways. By including sulfur-to-selenium mass shifts in a proteomic database search, we provide the first evidence of the dynamic and large-scale incorporation of selenocysteine as a response to oxidative stress in hydrogenotrophic methanogenesis and the presence of a dynamic selenoproteome.

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Section: ■ Results and Discussionmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Kleikamp,

Palacios,

Kofoed

et al. 2024

Environ. Sci. Technol.

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“…The addition of air into an anaerobic digester is used in this technique [ 53 ]. The method enhances sulfide oxidation by introducing oxygen in the reactor containing sludge under micro-aerobic conditions [ 54 ]. This method uses oxygen as an electron acceptor during sulfide oxidation, in which excess sulfide is further transformed into elemental sulfur, as shown in equation (1) .…”

Section: Physicochemical Proceduresmentioning

confidence: 99%

Approaches to mitigation of hydrogen sulfide during anaerobic digestion process – A review

Mutegoa,

Sahini

2023

Heliyon

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“…Another promising modification of anaerobic digesters is microaeration of the digestate to encourage the growth of aerobic and facultatively anaerobic microorganisms, particularly bacteria and fungi, to improve the digestion of complex polymers such as lignans, lignin, and β-linked polysaccharides (e.g., cellulose) that are normally resistant to degradation in anaerobic environments [ 15 , 16 ]. Microaeration can also reduce the concentration of H 2 S in biogas [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Microbiome Diversity of Anaerobic Digesters Is Enhanced by Microaeration and Low Frequency Sound

Loughrin,

Parekh,

Agga

et al. 2023

Microorganisms

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Biogas is produced by a consortium of bacteria and archaea. We studied how the microbiome of poultry litter digestate was affected by time and treatments that enhanced biogas production. The microbiome was analyzed at six, 23, and 42 weeks of incubation. Starting at week seven, the digesters underwent four treatments: control, microaeration with 6 mL air L−1 digestate per day, treatment with a 1000 Hz sine wave, or treatment with the sound wave and microaeration. Both microaeration and sound enhanced biogas production relative to the control, while their combination was not as effective as microaeration alone. At week six, over 80% of the microbiome of the four digesters was composed of the three phyla Actinobacteria, Proteobacteria, and Firmicutes, with less than 10% Euryarchaeota and Bacteroidetes. At week 23, the digester microbiomes were more diverse with the phyla Spirochaetes, Synergistetes, and Verrucomicrobia increasing in proportion and the abundance of Actinobacteria decreasing. At week 42, Firmicutes, Bacteroidetes, Euryarchaeota, and Actinobacteria were the most dominant phyla, comprising 27.8%, 21.4%, 17.6%, and 12.3% of the microbiome. Other than the relative proportions of Firmicutes being increased and proportions of Bacteroidetes being decreased by the treatments, no systematic shifts in the microbiomes were observed due to treatment. Rather, microbial diversity was enhanced relative to the control. Given that both air and sound treatment increased biogas production, it is likely that they improved poultry litter breakdown to promote microbial growth.

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A review of the practical application of micro-aeration and oxygenation for hydrogen sulfide management in anaerobic digesters

Cited by 17 publications

References 66 publications

The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

The Selenoproteome as a Dynamic Response Mechanism to Oxidative Stress in Hydrogenotrophic Methanogenic Communities

Approaches to mitigation of hydrogen sulfide during anaerobic digestion process – A review

Microbiome Diversity of Anaerobic Digesters Is Enhanced by Microaeration and Low Frequency Sound

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