A Review of Various Techniques of Mouse Liver Transplantation

Chen, J.; Gong, Weihua; Ge, Fan; Huang, Tao; Wu, Dang; Liang, Tingbo

doi:10.1016/j.transproceed.2013.03.035

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…The rat model of OLT and P-OLT is superior in regard to its technical feasibility and concerning the enormous amount of literature available when compared to the mouse models [117]. Supported by its feasibility and the favorable properties detailed above, it can be concluded that rat OLT (and P-OLT) is an indispensable model for transplantation research.…”

Section: Place Of Rat Olt and P-olt Models In Transplantation Researchmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Section: Place Of Rat Olt and P-olt Models In Transplantation Researchmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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“…(7,9) The portal clamping time was constantly within 15 minutes, with a median at 12.5 6 2.0 minutes (mean, 12.2 minutes). Such a time is significantly below the previously published ones, where the portal clamping is 16-17 minutes, (7,10,11) with the best series at 14.5 6 1.5 minutes. (12) The difference was explained by a faster QL-assisted portal cuff insertion, whereas the suprahepatic vena cava anastomosis time was similar to previous studies (7.0 6 1.2 minutes versus 8-10 minutes).…”

Section: Ql Reduces Portal Clamping Time and Allows For A Wider Portamentioning

confidence: 59%

“…This allows for the use of a wider cuff, with all the associated hemodynamic benefits. Compared with previous reports, where 20 G stents were used for the portal anastomosis, we were able to fit an 18 G cuff, which corresponds to a 96%‐99% gain in surface (depending on the Venflon brand used).…”

Section: Resultsmentioning

confidence: 78%

“…(12) The difference was explained by a faster QL-assisted portal cuff insertion, whereas the suprahepatic vena cava anastomosis time was similar to previous studies (7.0 6 1.2 minutes versus 8-10 minutes). (11) As already shown for rat liver transplantation, the QL provides the support for an optimal opening of the recipient vessels during the phase of implantation. (4) This allows for the use of a wider cuff, with all the associated hemodynamic benefits.…”

Section: Ql Reduces Portal Clamping Time and Allows For A Wider Portamentioning

confidence: 86%

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Efficient nonarterialized mouse liver transplantation using 3‐dimensional–printed instruments

et al. 2016

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Because of the wide availability of genetically modified animals, mouse orthotopic liver transplantation is often preferred over rat liver transplantation. We present a simplified mouse liver transplantation technique and compare transplantation outcomes with versus without hepatic artery anastomosis. Instruments for liver implantation were designed and printed with a 3-dimensional (3D) printer. The suprahepatic vena cava anastomosis was performed with a 10-0 running suture. The vena porta and infrahepatic vena cava were joined on extraluminal cuffs, using the 3D-printed device for spatial alignment and stabilization. The hepatic artery was reconstructed in half of the recipients using intraluminal stents. Liver function tests (3, 7, and 28 days) and histology (7 and 28 days) were assessed after transplantation. We performed 22 consecutive syngeneic C57BL/6 mouse orthotopic liver transplantations. The median portal clamping time was 12.5 ± 1.5 minutes. The survival rate at 4 weeks was 100% for both arterialized and nonarterialized recipients (n = 7, 4 recipients of each group being killed for early histology at day 7). Liver function tests at 3, 7, and 28 days were similar between arterialized versus nonarterialized groups. Liver parenchyma demonstrated only irrelevant abnormalities in both groups. The proposed device allows for a shorter clamping time compared with the published literature. Using this technique, the artery does not need to be anastomosed, with no impact on graft and recipient outcomes. The device is available for 3D printing. Liver Transplantation 22 1688-1696 2016 AASLD.

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“…In our study, we tried to use a methodology with potential clinical use in a reproducible ex vivo model of I/R, avoiding the complexity and the possible interference in the results related to the high mortality rate of the transplanted animal models [ 34 ]. We attempted to closely reproduce in the animal model the reality of the human liver transplantation and tried to understand the influence of adding BBR in the preservation solution, and this relates to mitochondrial function, bioenergetics and the impact in the liver histology.…”

Section: Discussionmentioning

confidence: 99%

Addition of Berberine to Preservation Solution in an Animal Model of Ex Vivo Liver Transplant Preserves Mitochondrial Function and Bioenergetics from the Damage Induced by Ischemia/Reperfusion

Martins

Rolo

Furtado

et al. 2018

IJMS

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Liver transplantation is a therapeutic regimen to treat patients with non-malignant end-stage liver diseases and malignant tumors of hepatic origin. The ischemia/reperfusion (I/R) injury in liver transplantation is associated with disruption of mitochondrial function in the hepatic parenchyma. Several studies have been conducted in animal models to identify pharmacological therapeutic strategies to minimize the injury induced by the cold/warm I/R in liver transplantation. Most of these studies were conducted in unrealistic conditions without the potential to be translated to clinical usage. Berberine (BBR) is a pharmacological compound with a potential protective effect of the mitochondrial function in the context of I/R. For the future clinical application of these pharmacological strategies, it is essential that a close resemblance exists between the methodology used in the animals models and real life. In this study, we have demonstrated that the addition of BBR to the preservation solution in an I/R setting preserves mitochondrial function and bioenergetics, protecting the liver from the deleterious effects caused by I/R. As such, BBR has the potential to be used as a pharmacological therapeutic strategy.

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A Review of Various Techniques of Mouse Liver Transplantation

Cited by 13 publications

References 25 publications

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Efficient nonarterialized mouse liver transplantation using 3‐dimensional–printed instruments

Addition of Berberine to Preservation Solution in an Animal Model of Ex Vivo Liver Transplant Preserves Mitochondrial Function and Bioenergetics from the Damage Induced by Ischemia/Reperfusion

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