In marine, aerospace, automotive, and biomedical engineering, components and structures are made of different materials that have properties in order to meet specific performance requirements. Stainless steel 316 and low alloy steels are the most commonly used materials in the marine industry, where corrosive resistivity and high specific strength are the design requirements. This was the main importance of welding dissimilar metal using automated MIG to increase productivity, improve quality, reliability, and reduce labor costs. This research work aimed to study how different MIG process parameters (welding current, wire feed rate, gas flow rate, and welding speed) affect the mechanical properties of welding two different metals together, namely stainless steel 316 and low alloy steel 4140. To study the significance and contribution of each parameter, analysis of variance (ANOVA) were conducted using Minitab 21 software. The tensile strength and flexural strength experimental results showed that the welding current parameter has a high significant effect in both cases, followed by welding speed. The hardness result shows that the welding speed followed by the welding current has a significant effect on hardness. Weld metal had higher hardness than stainless steel and low alloy steel base metals.