A review on analytical methods for natural berberine alkaloids

Wang, Jiahui; Jiang, Yanyan; Wang, Bing; Zhang, Ning

doi:10.1002/jssc.201800952

Cited by 29 publications

(14 citation statements)

References 108 publications

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“…With respect to the hypoglycemic activity, Pearson correlation coefficients of COL, JAT, EPI, COP, PAL, BER were 0.807 ( p < 0.01), 0.765 ( p < 0.01), 0.692 ( p < 0.01), 0.727 ( p < 0.01), 0.772 ( p < 0.01), 0.833 ( p < 0.01), respectively. These results were in agreement with previous pharmacological reports, 12,21–25 demonstrating that the six Coptis alkaloids were all responsible for the curative effects of CR and could be taken as quality control markers for further consistency evaluation.…”

Section: Resultssupporting

confidence: 92%

Consistency evaluation between dispensing granule and traditional decoction from Coptidis Rhizoma by using an integrated quality‐based strategy

Zhang

et al. 2020

Phytochemical Analysis

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Introduction Dispensing granule, an innovative product of traditional Chinese medicine decoction, is widely practiced in clinic. As a prerequisite to support the clinical medication, quality consistency between dispensing granule and traditional decoction need to be evaluated. Furthermore, a generally applicable strategy for consistency evaluation of dispensing granule is needed. Objective In this study, we aimed to propose an integrated quality‐based strategy to assess consistency between dispensing granule and traditional decoction taking Coptidis Rhizoma (CR) as a case study. Methodology For chemical consistency evaluation, efficacy‐related Coptis alkaloids were quantified with high‐performance liquid chromatography (HPLC). The “Mean ± 3SD” of analyte contents in traditional decoction was considered as the criterion of consistency. And, as auxiliary analysis, principal component analysis (PCA) was employed for data visualisation. For biological consistency evaluation, two one‐side t‐tests and 90% confidence intervals of the geometric mean ratio of antibacterial zone diameter and 50% inhibitory concentration (IC50) of α‐glucosidase inhibition were calculated. The scope of 80.00% to 125.00% was taken as in vitro bioequivalence interval. It was considered internally consistent with traditional decoction when the chemical and biological indices of dispensing granule fulfilled the preset criteria simultaneously. Results Eight out of 20 batches of CR dispensing granule were demonstrated consistent with traditional decoction in chemistry and biological activities. Conclusions A generally applicable strategy was recommended that integrates chemical and biological characteristics for consistency evaluation of dispensing granule.

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Section: Resultssupporting

confidence: 92%

Consistency evaluation between dispensing granule and traditional decoction from Coptidis Rhizoma by using an integrated quality‐based strategy

Zhang

et al. 2020

Phytochemical Analysis

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“…Coptidis rhizome is a traditional Chinese herb that has been widely used to treat damp heat and toxicity for over 2000 years . It is also well‐known for its cholesterol‐lowering, blood pressure lowering, antibacterial, antioxidant, anti‐inflammatory and anticancer effects . Coptidis rhizome attracts increasing interest in the scientific community because of its bioactive property.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Alkaloids from Coptidis Rhizoma via Betaine‐Based Deep Eutectic Solvents

Hsieh

Pan

et al. 2020

ChemistrySelect

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To avoid the use of volatile organic compounds, a series of betaine-based deep eutectic solvents (DESs), which are considered as green solvents, were prepared for the efficient extraction of alkaloids from coptidis rhizome. The extraction conditions were optimized via response surface methodology (RSM) to achieve the maximum recovery of total alkaloids. A central composite design with three independent variables which included the concentration of the selected DES, extrac-tion time, and solvent/solid ratio at five levels, was applied while response surface plots were produced with a second order polynomial model. A one-off experiment was performed to examine the reliability of the simulated results obtained from RSM. An optimized yield of 128.43 mg/g was obtained at an extraction time of 29.5 min and a solvent/solid ratio of 35 : 1 with the extraction solvent as 0.6 M betaine-tartaric acid-water DES.

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“…The mass difference between the formulae of the molecular cations of compound 13 and berberrubine was 30 Da (CH 2 O), suggesting that there was one more –OCH 3 group in the structure of compound 13 than in that of berberrubine. By comparing the biosynthetic pathway of isoquinoline alkaloids with compounds reported in the literature, 25,33,34 the –OCH 3 substituent was assigned to the C 13 ‐positon, and compound 13 was identified as 13‐methoxyberberrubine. In a similar way, compounds 15 , 17 and 25 were identified as 13‐methoxyberberine, columbamine and 13‐methylberberine, respectively.…”

Section: Resultsmentioning

confidence: 99%

Systematic characterization of alkaloids in Eomecon chionantha Hance using ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry with a four‐step screening strategy

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Eomecon chionantha Hance (ECH), a traditional folk herb, is commonly used to treat traumatic injuries based on its analgesic and anti‐inflammatory properties. Previous studies have reported that alkaloids are the major bioactive components in ECH. Therefore, identification of alkaloids from ECH contributes to the discovery of its potential active ingredients and quality control in clinic treatments. Methods A four‐step screening strategy was performed as follows. (1) Extracting the accurate masses of ions related to different molecules. (2) Screening different types of compounds using their molecular cations, protonated molecules, diagnostic product ions and fragmentation pathways. (3) Comparing the characteristic product ion formulae to obtain the type and number of substituents. (4) Using the biosynthetic pathways of isoquinoline alkaloids to determine the concentration of alkaloids. Results Ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry (UHPLC/Q‐Exactive Orbitrap MS) analysis combined with the four‐step screening strategy was used to profile the alkaloids in ECH. The structures of 95 alkaloids in ECH were unambiguously identified or reasonably assigned, of which 76 were reported in ECH for the first time. Six types of benzylisoquinoline alkaloids were identified in ECH: six benzyltetrahydroisoquinolines, nine protopines, five N‐methyltetrahydroprotoberberines, six protoberberines, eight benzophenanthridines and sixty‐one dihydrobenzophenanthridines. Conclusions This comprehensive study identified the alkaloids in ECH, thus providing a practical reference for further research. The UHPLC/Q‐Exactive Orbitrap MS method, combined with the four‐step screening strategy, which was developed and successfully applied to identify the alkaloids in ECH, may also be applicable for the efficient screening of other herbal medicines.

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A review on analytical methods for natural berberine alkaloids

Cited by 29 publications

References 108 publications

Consistency evaluation between dispensing granule and traditional decoction from Coptidis Rhizoma by using an integrated quality‐based strategy

Consistency evaluation between dispensing granule and traditional decoction from Coptidis Rhizoma by using an integrated quality‐based strategy

Extraction of Alkaloids from Coptidis Rhizoma via Betaine‐Based Deep Eutectic Solvents

Systematic characterization of alkaloids in Eomecon chionantha Hance using ultrahigh‐performance liquid chromatography–tandem quadrupole Exactive Orbitrap mass spectrometry with a four‐step screening strategy

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