A review on antibacterial silk fibroin-based biomaterials: current state and prospects

Ghalei, Sama; Handa, Hitesh

doi:10.1016/j.mtchem.2021.100673

Cited by 61 publications

(42 citation statements)

References 205 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deconvolution was performed using Pseudo-Voigt functions wi tions identified and the area percentage of the component band obtained, as l 1. The d spacings were assigned according to the previous literature [13,19,4 The results confirmed that both SF and SSF had prevalent silk II structu fested by the area percentage (relative proportion). For SSF, the relative prop II structure reached 71.3%.…”

Section: Structure Characteristicssupporting

confidence: 52%

“…Silk fibers from the Bombyx mori silkworm are composed mainly of sericin and silk fibroin (SF). SF, as a natural fibrous protein, is a promising biomaterial for drug delivery matrices, coatings for cell culture, and scaffolds for tissue engineering due to its excellent biocompatibility, robust mechanical properties, and intrinsic biodegradability [1][2][3][4][5][6][7][8][9][10][11]. These properties can be attributed to the unique structure of the protein itself.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical Modification of Silk Fibroin through Serine Amino Acid Residues

et al. 2022

View full text Add to dashboard Cite

Silk fibroin (SF) is a natural protein polymer and promising biomaterial. Chemical modifications have attracted growing interest in expanding SF applications. However, the majority of amino acid residues in SF are non-reactive and most of the reactive ones are in the crystalline region. Herein, a modification was conducted to investigate the possibility of direct modification on the surface of natural SF by a reagent with a mild reactivity, the type and quantity of the residues involved in the reactions, and the structural changes upon modification. Infrared spectrum, 1H NMR, titration and amino acid analyses, X-ray diffraction, and hemolysis test were used to analyze the materials. The results showed that sulfonic acid groups were grafted onto SF and the reaction occurred mainly at serine residues through hydroxyl groups. In total, 0.0958 mmol/g of residues participated in the modification with a modification efficiency of 7.6%. Moreover, the crystallinity and the content of β-sheet structure in SF increased upon modification. The modified material had good blood-compatibility. In conclusion, surface modification on native SF through serine residues was practicable and had the advantage of increased β-sheet structure. This will provide an alternative way for the modification of fibroin for the desired application in the biomedical field.

show abstract

Section: Structure Characteristicssupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Chemical Modification of Silk Fibroin through Serine Amino Acid Residues

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Currently, there are various processing forms for SF: scaffolds, sponges, or films, etc. [ 35 , 36 , 37 , 38 , 39 , 40 ]. It has been shown that the biocompatibility feature is influenced by the purification method and the purification process.…”

Section: Sf Key Propertiesmentioning

confidence: 99%

The Contribution of Silk Fibroin in Biomedical Engineering

Lujerdean

Baci

Cucu

et al. 2022

Insects

View full text Add to dashboard Cite

Silk fibroin (SF) is a natural protein (biopolymer) extracted from the cocoons of Bombyx mori L. (silkworm). It has many properties of interest in the field of biotechnology, the most important being biodegradability, biocompatibility and robust mechanical strength with high tensile strength. SF is usually dissolved in water-based solvents and can be easily reconstructed into a variety of material formats, including films, mats, hydrogels, and sponges, by various fabrication techniques (spin coating, electrospinning, freeze-drying, and physical or chemical crosslinking). Furthermore, SF is a feasible material used in many biomedical applications, including tissue engineering (3D scaffolds, wounds dressing), cancer therapy (mimicking the tumor microenvironment), controlled drug delivery (SF-based complexes), and bone, eye and skin regeneration. In this review, we describe the structure, composition, general properties, and structure–properties relationship of SF. In addition, the main methods used for ecological extraction and processing of SF that make it a green material are discussed. Lastly, technological advances in the use of SF-based materials are addressed, especially in healthcare applications such as tissue engineering and cancer therapeutics.

show abstract

“…With the gradual development and application of the efficacy of fibroin in silk, silk has been favored in biomedicine as a valuable natural protein fiber raw material [ 6 ]. Like natural silk fibers, the main components of cocoon waste and waste fabrics are silk fibroin and sericin, which can be used as raw materials for the development of silk-based products [ 7 ]. Therefore, recycled silk waste still has a considerable recycling value left to be explored and broad application prospects in the field of medical hydrogels ( Scheme 1 A).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery

et al. 2022

View full text Add to dashboard Cite

In recent years, hydrogels have been widely used as drug carriers, especially in the area of protein delivery. The natural silk fibroin produced from cocoons of the Bombyx mori silkworm possesses excellent biocompatibility, significant bioactivity, and biodegradability. Therefore, silk fibroin-based hydrogels are arousing widespread interest in biomedical research. In this study, a process for extracting natural silk fibroin from raw silk textile yarns was established, and three aqueous solutions of silk fibroin with different molecular weight distributions were successfully prepared by controlling the degumming time. Silk fibroin was dispersed in the aqueous solution as “spherical” aggregate particles, and the smaller particles continuously accumulated into large particles. Finally, a silk fibroin hydrogel network was formed. A rheological analysis showed that as the concentration of the silk fibroin hydrogel increased its storage modulus increased significantly. The degradation behavior of silk fibroin hydrogel in different media verified its excellent stability, and the prepared silk fibroin hydrogel had good biocompatibility and an excellent drug-loading capacity. After the protein model drug BSA was loaded, the cumulative drug release within 12 h reached 80%. We hope that these investigations will promote the potential utilities of silk fibroin hydrogels in clinical medicine.

show abstract

A review on antibacterial silk fibroin-based biomaterials: current state and prospects

Cited by 61 publications

References 205 publications

Chemical Modification of Silk Fibroin through Serine Amino Acid Residues

Chemical Modification of Silk Fibroin through Serine Amino Acid Residues

The Contribution of Silk Fibroin in Biomedical Engineering

Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery

Contact Info

Product

Resources

About