A Review on Biological Synthesis of the Biodegradable Polymers Polyhydroxyalkanoates and the Development of Multiple Applications

Bryan, Dalton; Bhagabati, Purabi; Micco, Jessica De; Padamati, Ramesh Babu; O’Connor, Kevin E.

doi:10.3390/catal12030319

Cited by 99 publications

(54 citation statements)

References 304 publications

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“…The existence of a wide range of PHA-producing microorganisms capable of using various C-substrates for growth is the basis for obtaining various types of PHA with different valuable properties for various applications [ 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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Synthesis of P(3HB-co-3HV-co-4HV) copolymers by the wild-type strain Cupriavidus necator B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA,DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (Cx 38–49%) molecular weight characteristics Mn (45–87 kDa), and Mw (201–248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB-co-3HV-co-4HV) copolymers using Cupriavidus necator B-10646.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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“…Hfx. mediterranei , in particular, is able to synthesize the PHBV copolymer ( Han et al, 2015 ) which, compared to the PHB homopolymer, exhibits improved physical properties, which are function of the HV content ( Dalton et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater

et al. 2022

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The use of the halophile microorganism Haloferax mediterranei, able to synthesize poly(hydroxybutyrate-hydroxyvalerate) (PHBV), is considered as a promising tool for the industrial production of bioplastic through bioprocessing. A consistent supplementation of the growth substrate in carbohydrates and minerals is overall necessary to allow its PHBV production. In this work, wasted bread was used as substrate for bioplastic production by microbial fermentation. Instead of the consistent and expensive minerals supplement required for Hfx. mediterranei DSM1411 growth, microfiltered seawater was added to the wasted bread-derived substrate. The suitable ratio of wasted bread homogenate and seawater, corresponding to 40:60, was selected. The addition of proteases and amylase to the bread homogenate promoted the microbial growth but it did not correspond to the increase of bioplastic production by the microorganism, that reach, under the experimental conditions, 1.53 g/L. An extraction procedure of the PHBV from cells, based on repeated washing with water, followed or not by a purification through ethanol precipitation, was applied instead of the conventional extraction with chloroform. Yield of PHBV obtained using the different extraction methods were 21.6 ± 3.6 (standard extraction/purification procedure with CHCl3:H2O mixture), 24.8 ± 3.0 (water-based extraction), and 19.8 ± 3.3 mg PHAs/g of wasted bread (water-based extraction followed by ethanol purification). Slightly higher hydroxyvalerate content (12.95 vs 10.78%, w/w) was found in PHBV obtained through the water-based extraction compared to the conventional one, moreover, the former was characterized by purity of 100% (w/w). Results demonstrated the suitability of wasted bread, supplemented with seawater, to be used as substrate for bioplastic production through fermentation. Results moreover demonstrated that a solvent-free extraction, exclusively based on osmotic shock, could be used to recover the bioplastic from cells.

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“…The capacity to breakdown low-density polyethylene (LDPE) used in widely available plastic carry bags was nearly 8% [ 19 ]. Since synthetic polymers are regarded as practically inert, efforts to isolate specific bacteria capable of exploiting them were discussed in this review [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

et al. 2022

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Biodegradation is necessary for water-soluble or water-immiscible polymers because they eventually enter streams which can neither be recycled nor incinerated. It is important to consider the microbial degradation of natural and synthetic polymers in order to understand what is necessary for biodegradation and the mechanisms involved. Low/high-density polyethylene is a vital cause of environmental pollution. It occurs by choking the sewer line through mishandling, thus posing an everlasting ecological threat. Environmental pollution due to the unscrupulous consumption of synthetic polymers derived from petroleum has an adverse impact on the environment since the majority of plastics do not degrade, and the further incineration of synthetic plastics generates CO2 and dioxin. This requires understanding the interactions between materials and microorganisms and the biochemical changes involved. Widespread studies on the biodegradation of plastics have been carried out in order to overcome the environmental problems associated with synthetic plastic waste. Awareness of the waste problem and its impact on the environment has awakened new interest in the area of degradable polymers through microbes viz., bacteria, fungi, and actinomycetes. The microbial degradation of plastics is caused by certain enzymatic activities that lead to a chain cleavage of polymers into oligomers and monomers. This review focuses on the biodegradation rate of plastics by fungal and bacterial communities and the mode of action of biodegradation.

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A Review on Biological Synthesis of the Biodegradable Polymers Polyhydroxyalkanoates and the Development of Multiple Applications

Cited by 99 publications

References 304 publications

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Exploitation of wasted bread as substrate for polyhydroxyalkanoates production through the use of Haloferax mediterranei and seawater

Novel Approach in Biodegradation of Synthetic Thermoplastic Polymers: An Overview

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