A review on colorimetric assays for DNA virus detection

Abdolhosseini, Mansoreh; Zandsalimi, Farshid; Moghaddam, Fahimeh Salasar; Tavoosidana, Gholamreza

doi:10.1016/j.jviromet.2022.114461

Cited by 12 publications

(5 citation statements)

References 141 publications

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“…Modern-day microfluidic technology has evolved tremendously over the last several decades, serving various functions and spanning multiple industries. Although microfluidic technology has demonstrated its historical significance across many fields, its most relevant function in contemporary society lies in its diagnostic applications [14][15][16][17][18]. Several key properties of contemporary microfluidic devices originated from the industries for which microfluidics were originally designed, including molecular analysis, biodefense, molecular biology, and microelectronics.…”

Section: Past Rna Diagnosticsmentioning

confidence: 99%

“…As a result, diagnostic tests with very small turnaround time and short production times were quickly prioritized for research and development. Qualitative diagnostic tests such as the rapid antigen tests developed throughout the first two years of the pandemic have allowed many people to diagnose themselves and slow down the progression and continued evolution of SARS-CoV-2 variants more readily but did not stop it [17]. As a result, many people in more resource-wealthy countries around the world have had an increased chance of returning to some sense of normalcy in their daily lives [18].…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic

Escobar,

Diab-Liu,

Bosland

et al. 2023

Biosensors

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The global economic and healthcare crises experienced over the past three years, as a result of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has significantly impacted the commonplace habits of humans around the world. SARS-CoV-2, the virus responsible for the coronavirus 2019 (COVID-19) phenomenon, has contributed to the deaths of millions of people around the world. The potential diagnostic applications of microfluidic devices have previously been demonstrated to effectively detect and quasi-quantify several different well-known viruses such as human immunodeficiency virus (HIV), influenza, and SARS-CoV-2. As a result, microfluidics has been further explored as a potential alternative to our currently available rapid tests for highly virulent diseases to better combat and manage future potential outbreaks. The outbreak management during COVID-19 was initially hindered, in part, by the lack of available quantitative rapid tests capable of confirming a person’s active infectiousness status. Therefore, this review will explore the use of microfluidic technology, and more specifically RNA-based virus detection methods, as an integral part of improved diagnostic capabilities and will present methods for carrying the lessons learned from COVID-19 forward, toward improved diagnostic outcomes for future pandemic-level threats. This review will first explore the context of the COVID-19 pandemic and how diagnostic technology was shown to have required even greater advancements to keep pace with the transmission of such a highly infectious virus. Secondly, the historical significance of integrating microfluidic technology in diagnostics and how the different types of genetic-based detection methods may vary in their potential practical applications. Lastly, the review will summarize the past, present, and future potential of RNA-based virus detection/diagnosis and how it might be used to better prepare for a future pandemic.

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Section: Past Rna Diagnosticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic

Escobar,

Diab-Liu,

Bosland

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…These molecules are submitted to a unidirectional flow in a liquid medium that promotes their interaction with capture molecules immobilized on a membrane, which is part of test strip [3]. Colorimetry is the most popular physicochemical detection approach applied in NALFA, usually using gold nanoparticles (AuNPs) as reporter molecules [6]. AuNPs are generally attached to 1) antibodies targeting specific antigens on the extremities of amplicons, or 2) oligonucleotide probes that hybridize with ssDNA amplicon´products [3].…”

Section: Introductionmentioning

confidence: 99%

The potential of tailed amplicons for SARS-CoV-2 detection in Nucleic Acid Lateral Flow Assays

Vindeirinho,

Oliveira,

Pinho

et al. 2024

PLoS ONE

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Nucleic Acid Lateral Flow Assays (NALFAs) are a promising solution for the point-of-care detection of viruses like SARS-CoV-2. However, they show some drawbacks, such as the great dependency on the use of antibodies and the need for post-amplification protocols that enable the preparation of amplicons for effective readings, as well as low sensitivity. Here, we developed amplicons of a specific SARS-CoV-2 gene tailed with single-strand DNA (ssDNA) sequences to hybridize with DNA probes immobilized on the NALFA strips, thus overcoming the aforementioned problems. Results have shown that tailed primers have not compromised the amplification efficiency and allowed the correct detection of the amplicons in the lateral flow strip. This approach has presented a limit of detection (LOD) of 25 RNA copies /reaction mix (1 copy/μL) and the test of cross-reactivity with other related viruses has not shown any cross-reactivity. Twenty clinical samples were evaluated by NALFA and simultaneously compared with the gold standard RT-qPCR protocol, originating equal results. Although the number of clinical specimens tested being relatively small, this indicates a sensitivity and specificity both of 100%. In short, an alternative NALFA was successfully implemented, rendering an accurate route for SARS-CoV-2 diagnosis, compatible with low-resource settings.

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“…The existing methods, such as virus culture, polymerase chain reaction (PCR) [10] , enzyme linked immunosorbent assay (ELISA) [11] and other serological methods [12] , are widely used in clinical practice. These methods are extremely sensitive and specific, however, they require expensive equipment and professional operators and suffer from major drawbacks of being time consuming and labor intensive [13,14] .…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized semiconductor quantum dots for virus detection

Liang¹,

Mao²,

Dai³

et al. 2023

J. Semicond.

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Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid, which must be replicated using the host cell system. It causes large-scale infectious diseases and poses serious threats to the health, social well-being, and economic conditions of millions of people worldwide. Therefore, there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission. Quantum dots (QDs) are typical fluorescence nanomaterials with high quantum yield, broad absorbance range, narrow and size-dependent emission, and good stability. QDs-based nanotechnology has been found to be effective method with rapid response, easy operation, high sensitivity, and good specificity, and has been widely applied for the detection of different viruses. However, until now, no systematic and critical review has been published on this important research area. Hence, in this review, we aim to provide a comprehensive coverage of various QDs-based virus detection methods. The fundamental investigations have been reviewed, including information related to the synthesis and biofunctionalization of QDs, QDs-based viral nucleic acid detection strategies, and QDs-based immunoassays. The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.

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A review on colorimetric assays for DNA virus detection

Cited by 12 publications

References 141 publications

Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic

Microfluidic Device-Based Virus Detection and Quantification in Future Diagnostic Research: Lessons from the COVID-19 Pandemic

The potential of tailed amplicons for SARS-CoV-2 detection in Nucleic Acid Lateral Flow Assays

Biofunctionalized semiconductor quantum dots for virus detection

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