A review on design-build-test-learn cycle to potentiate progress in isoprenoid engineering of photosynthetic microalgae

Li, Xiangyu; Lan, Chengxiang; Hu, Zhangli; Jia, Bin

doi:10.1016/j.biortech.2022.127981

Cited by 8 publications

(3 citation statements)

References 123 publications

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“…In metabolic engineering, degron-induced degradation systems are often employed by adding degrons to target proteins that exhibit high expression levels in host cells to reduce the burden of excessive protein accumulation, with the goal of maximizing production yield. These synthetic circuits alleviate the cellular burden caused by the accumulation of heterologous proteins and address imbalances in mass-energy equilibrium within the cell [28][29][30][31].…”

Section: Biosensors For Controlling Cellular Protein Abundance and Me...mentioning

confidence: 99%

“…Further iteration of chassis development, or perhaps total redesign, is required to improve yield. Engineering biological systems is generally a sophisticated process that requires potentially costly Design-Build-Test-Learn (DBTL) cycles to iteratively seek the optimal system (Figure 1) [28,29]. The test phase, which evaluates the performance of the constructed biological system for example, determining the amount of the desired chemical produced, is often particularly laborious, costly, and time-consuming [30,31].…”

Section: Introductionmentioning

confidence: 99%

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State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering

Chaisupa,

Wright

2023

Preprint

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Genetically encoded biosensors are crucial for enhancing our understanding of how molecules regulate biological systems. Small molecule biosensors, in particular, help us understand the interaction between chemicals and biological processes. They also accelerate metabolic engineering by increasing screening throughput and eliminating the need for sample preparation through traditional chemical analysis. Additionally, they offer significantly higher spatial and temporal resolution in cellular analyte measurements. In this review, we discuss recent progress in in vivo biosensors and control systems—biosensor-based controllers—in metabolic engineering. We also explore specifically protein-based biosensors that utilize less commonly exploited signaling mechanisms, such as protein stability and induced degradation, compared to more prevalent transcription factor and allosteric regulation mechanism. We proposed that these lesser-used mechanisms will be significant for engineering eukaryotic systems and slower-growing prokaryotic systems where protein turnover may facilitate more rapid and reliable measurement and regulation of the current cellular state. Lastly, we emphasize the utilization of cutting-edge and state-of-the-art techniques in the development of protein-based biosensors, achieved through rational design, directed evolution, and collaborative approaches.

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Section: Biosensors For Controlling Cellular Protein Abundance and Me...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering

Chaisupa,

Wright

2023

Preprint

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“…Recently, eukaryotic microalgae have emerged as sustainable alternatives for biotechnological production processes [ 13 , 14 , 15 ]. Light-driven photosynthetic microbes, such as Chlamydomonas reinhardtii can offer the potential for sustainable production processes [ 16 , 17 ]. Algal cells are ideal hosts for the production of heterologous plant terpenoids owing to their shared distant evolutionary ancestry with land plants and their cultivation temperatures similar to those used for plant growth [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Promoting Photosynthetic Production of Dammarenediol-II in Chlamydomonas reinhardtii via Gene Loading and Culture Optimization

Zhao

Lan

et al. 2023

IJMS

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Ginsenosides are major bioactive compounds found in Panax ginseng that exhibit various pharmaceutical properties. Dammarenediol-II, the nucleus of dammarane-type ginsenosides, is a promising candidate for pharmacologically active triterpenes. Dammarenediol-II synthase (DDS) cyclizes 2,3-oxidosqualene to produce dammarenediol-II. Based on the native terpenoids synthetic pathway, a dammarane-type ginsenosides synthetic pathway was established in Chlamydomonas reinhardtii by introducing P. ginseng PgDDS, CYP450 enzyme (PgCYP716A47), or/and Arabidopsis thaliana NADPH-cytochrome P450 reductase gene (AtCPR), which is responsible for producing dammarane-type ginsenosides. To enhance productivity, strategies such as “gene loading” and “culture optimizing” were employed. Multiple copies of transgene expression cassettes were introduced into the genome to increase the expression of the key rate-limiting enzyme gene, PgDDS, significantly improving the titer of dammarenediol-II to approximately 0.2 mg/L. Following the culture optimization in an opt2 medium supplemented with 1.5 mM methyl jasmonate under a light:dark regimen, the titer of dammarenediol-II increased more than 13-fold to approximately 2.6 mg/L. The C. reinhardtii strains engineered in this study constitute a good platform for the further production of ginsenosides in microalgae.

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Omics Approaches for Algal Applications

Shah,

Dixit,

Elsayed

et al. 2023

Value-Added Products From Algae

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A review on design-build-test-learn cycle to potentiate progress in isoprenoid engineering of photosynthetic microalgae

Cited by 8 publications

References 123 publications

State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering

State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering

Promoting Photosynthetic Production of Dammarenediol-II in Chlamydomonas reinhardtii via Gene Loading and Culture Optimization

Omics Approaches for Algal Applications

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