A review on efforts for improvement in medicinally important chemical constituents inAconitum through biotechnological interventions

Tiwari, Shrikant; Acharya, Puja; Solanki, Bharat; Sharma, Anish Kumar; Rawat, Sandeep

doi:10.1007/s13205-023-03578-z

Cited by 5 publications

(2 citation statements)

References 189 publications

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“…To date, a total of 84 alkaloids have been obtained from TBC [ 6 ]. Diterpenoid alkaloids, such as aconitine and 3-acetylaconitine, are the characteristic compounds in most of the Aconitum species [ 7 ]. These phytochemicals have been shown to exhibit a wide range of pharmacological properties, such as dispelling cold, alleviating pain, and inhibiting bacteria, as well as treating injuries, rheumatic lumbar pain, and chilblains [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.

Sun,

et al. 2024

Plants

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The classification system for the genus Aconitum is highly complex. It is also the subject of ongoing debate. Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz. are perennial herbs of the genus Aconitum. Dried roots of these two plants are used in traditional Chinese medicine. In this study, morphological observations and ISSR molecular markers were employed to discriminate between A. flavum and A. pendulum, with the objective of gaining insights into the interspecies classification of Aconitum. The pubescence on the inflorescence of A. flavum was found to be appressed, while that on the inflorescence of A. pendulum was spread. UPGMA (unweighted pair-group method with arithmetic average) cluster analysis, PCoA (principal coordinates analysis), and Bayesian structural analysis divided the 199 individuals (99 individuals from DWM population and 100 individuals from QHL population) into two main branches, which is consistent with the observations of the morphology of pubescence on the inflorescence. These analyses indicated that A. flavum and A. pendulum are distinct species. No diagnostic bands were found between the two species. Two primer combinations (UBC808 and UBC853) were ultimately selected for species identification of A. flavum and A. pendulum. This study revealed high levels of genetic diversity in both A. flavum (He = 0.254, I = 0.395, PPB = 95.85%) and A. pendulum (He = 0.291, I = 0.445, PPB = 94.58%). We may say, therefore, that ISSR molecular markers are useful for distinguishing A. flavum and A. pendulum, and they are also suitable for revealing genetic diversity and population structure.

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Section: Introductionmentioning

confidence: 99%

Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.

Sun,

et al. 2024

Plants

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“…We recently had the pleasure of reading an informative review article titled "A Review on Efforts for Improvement In Medicinally Important Chemical Constituents In Aconitum Through Biotechnological Interventions" by Tiwari et al 1 , which appeared in 3 Biotech (https://www.springer.com/journal/13205). The research described in the article highlights the medicinal value and pharmacological activities of aconitine and other structurally similar compounds, as demonstrated previously 2,3,4,5 .…”

Section: Introductionmentioning

confidence: 99%

Effects of aconitine on membrane currents and action potentials in neonatal rat ventricular myocytes and its impact on electrocardiographic changes

Chou,

Cheung So

2024

MRAJ

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This study presents an analysis of the electrophysiological effects of aconitine, a toxic diterpenoid alkaloid derived from Aconitum plants, on neonatal rat ventricular myocytes (NRVMs). The research investigates the potential impact of aconitine on various ion currents and cardiac action potentials, shedding light on its arrhythmogenic properties. Whole-cell patch-clamp experiments were conducted to assess the effects of aconitine on delayed-rectifier K+ currents (IK(DR)) and inwardly rectifying K+ currents (IK(IR) in NRVMs. The findings indicate that aconitine exposure led to the inhibition of (IK(DR)) and (IK(IR)), suggesting its potential influence on cardiac repolarization and excitability. Notably, aconitine induced transient inward current (ITI) and early after-depolarizations (EADs) in a concentration- dependent manner, both of which have implications for cardiac arrhythmias. Moreover, the study examined the electrocardiogram changes in Sprague-Dawley rats upon aconitine injection, revealing a prolonged QT interval and the emergence of polymorphic ventricular tachycardia (VTs), indicative of arrhythmic effects. The study emphasizes the importance of understanding the electrophysiological impact of aconitine and similar compounds, considering their potential therapeutic applications and associated toxicities.

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Effect of Aconitum diphtheria on the Proliferation, Apoptosis, and Inflammatory Response of Rheumatoid Arthritis Fibroblast‐Like Synoviocytes

Meng,

Cai,

Cong

et al. 2024

Cell Biochemistry & Function

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Rheumatoid arthritis (RA), a highly disabling autoimmune disease, is characterized by joint damage and synovial hyperplasia. This paper was designed to explore the effect and mechanisms of Aconitum diphtheria on the proliferation and apoptosis of RA fibroblast‐like synoviocytes (RA‐FLS). First, RA‐FLS was treated with different doses of A. diphtheria extracts. Then, an inverted biological microscope was adopted to observe the RA‐FLS morphology. Subsequently, terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end labeling staining, Cell Counting Kit‐8 and western blot were utilized to analyze the apoptosis, proliferation, and nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) signaling pathway‐related proteins in RA‐FLS, respectively. The experimental results disclosed that, after treatment of RA‐FLS with A. diphtheria extracts, the cells became round and the intercellular space was increased. Besides, the RA‐FLS proliferation was effectively inhibited by A. diphtheria extracts, while the apoptosis was promoted. Additionally, A. diphtheria extracts could effectively downregulate the expression levels of p‐NF‐κB (p50), NF‐κB (p50), p‐NF‐κB (p65), and NF‐κB (p65). Collectively, A. diphtheria can effectively inhibit RA‐FLS proliferation and promote apoptosis in a dose‐dependent manner. Similarly, A. diphtheria is able to downregulate p‐NF‐κB and NF‐κB protein expression, but there is no dose–response relationship.

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A review on efforts for improvement in medicinally important chemical constituents inAconitum through biotechnological interventions

Cited by 5 publications

References 189 publications

Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.

Species Identification and Genetic Diversity Analysis of Medicinal Plants Aconitum pendulum Busch and Aconitum flavum Hand.-Mazz.

Effects of aconitine on membrane currents and action potentials in neonatal rat ventricular myocytes and its impact on electrocardiographic changes

Effect of Aconitum diphtheria on the Proliferation, Apoptosis, and Inflammatory Response of Rheumatoid Arthritis Fibroblast‐Like Synoviocytes

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