A review on factors affecting the Agrobacterium-mediated genetic transformation in ornamental monocotyledonous geophytes

Koetle, M.J.; Finnie, J.F.; Balázs, Ervin; Staden, J. Van

doi:10.1016/j.sajb.2015.02.001

Cited by 32 publications

(14 citation statements)

References 77 publications

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“…It seems that these two regeneration pathways often occurred in the same callus clumps. Embryogenic callus or somatic embryogenesis is considered to be one of the most efficient regeneration and transformation system for plants (Ceasar and Ignacimuthu 2011;Koetle et al 2015;Parimalan et al 2011). Type III callus, which was compact and whitish ( Figure 1C), was commonly observed in medium supplemented with 2,4-D alone, or in combination with 6-BA and NAA.…”

Section: Callus Induction From Leaf Explantsmentioning

confidence: 99%

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

Deng

Ouyang

et al. 2019

Plant Biotechnology

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Neolamarckia cadamba is a miracle tree species with considerable economic potential uses as a timber wood, woody forage and traditional medicine resource. The present study aimed to establish a highly efficient and robust protocol of plant regeneration for N. cadamba. Greenish callus was induced from very young leaf explants of sterile in vitro plantlets cultured on Murashige and Skoog's (MS) medium supplemented with 3 mg l −1 thidiazuron (TDZ), 0.1 mg l −1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.05 mg l −1 α-naphthaleneacetic acid (NAA). The callus could differentiate into nodular embryogenic structures or adventitious shoots, and these two regeneration pathways often occurred in the same callus clumps. The micro-shoots developed roots in MS supplemented with 0.05 mg l −1 NAA and 0.05 mg l −1 indole-3butyric acid (IBA), while the nodular embryogenic structures germinated directly and developed into plantlets on induction medium contained with 0.5 mg l −1 (or 1 mg l −1) 6-benzyladenine (6-BA) and 0.05 mg l −1 NAA. The rooted plantlets could be successfully acclimatized to a greenhouse with more than 92.0% survival. This regeneration protocol can be used in large scale cultivation needs and may be useful for future genetic modifications of N. cadamba.

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Section: Callus Induction From Leaf Explantsmentioning

confidence: 99%

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

Deng

Ouyang

et al. 2019

Plant Biotechnology

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“…Nevertheless, alternative direction involving the transformation of hop, utilizing either heterologous or homologous gene expression system may provide a promising approach for secondary metabolite engineering [ 25 ]. The genetic transformation technology, which is considered as an extension of conventional plant breeding technologies has been practiced in several plant species for the introduction of desirable agronomic traits from more than three decades and became an established technology to generate precise, rapid and stable modifications in utilized cultivars [ 26 , 27 ]. Over the past years, genetic engineering technology has been used for manipulation of secondary metabolite biosynthesis in different plant species via constitutive overexpression of homologous and heterologous transcription factors [ 28 – 30 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors

et al. 2018

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BackgroundThe hop plant (Humulus lupulus L.) is a valuable source of several secondary metabolites, such as flavonoids, bitter acids, and essential oils. These compounds are widely implicated in the beer brewing industry and are having potential biomedical applications. Several independent breeding programs around the world have been initiated to develop new cultivars with enriched lupulin and secondary metabolite contents but met with limited success due to several constraints. In the present work, a pioneering attempt has been made to overexpress master regulator binary transcription factor complex formed by HlWRKY1 and HlWDR1 using a plant expression vector to enhance the level of prenylflavonoid and bitter acid content in the hop. Subsequently, we performed transcriptional profiling using high-throughput RNA-Seq technology in leaves of resultant transformants and wild-type hop to gain in-depth information about the genome-wide functional changes induced by HlWRKY1 and HlWDR1 overexpression.ResultsThe transgenic WW-lines exhibited an elevated expression of structural and regulatory genes involved in prenylflavonoid and bitter acid biosynthesis pathways. In addition, the comparative transcriptome analysis revealed a total of 522 transcripts involved in 30 pathways, including lipids and amino acids biosynthesis, primary carbon metabolism, phytohormone signaling and stress responses were differentially expressed in WW-transformants. It was apparent from the whole transcriptome sequencing that modulation of primary carbon metabolism and other pathways by HlWRKY1 and HlWDR1 overexpression resulted in enhanced substrate flux towards secondary metabolites pathway. The detailed analyses suggested that none of the pathways or genes, which have a detrimental effect on physiology, growth and development processes, were induced on a genome-wide scale in WW-transgenic lines.ConclusionsTaken together, our results suggest that HlWRKY1 and HlWDR1 simultaneous overexpression positively regulates the prenylflavonoid and bitter acid biosynthesis pathways in the hop and thus these transgenes are presented as prospective candidates for achieving enhanced secondary metabolite content in the hop.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5125-8) contains supplementary material, which is available to authorized users.

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“…Raineri et al 1990 [75] published a paper on the production of the first stable monocot transgenic crop (rice) by Agrobacterium -mediated transformation. Later, Agrobacterium -mediated transformation methods were adopted by various monocotyledonous plants, from cereal crops to ornamental plants [76,77,78]. Similar to monocot plants, in vitro propagation of oil palm via somatic embryogenesis is an efficient approach.…”

Section: Genetic Transformation Of Oil Palmmentioning

confidence: 99%

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

Yarra

Jin

Zhao

et al. 2019

IJMS

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Oil palm (Elaeis guineensis, Jacq.) is a prominent vegetable-oil-yielding crop. Cultivating high-yielding oil palm with improved traits is a pre-requisite to meet the increasing demands of palm oil consumption. However, tissue culture and biotechnological approaches can resolve these concerns. Over the past three decades, significant research has been carried out to develop tissue culture and genetic transformation protocols for oil palm. Somatic embryogenesis is an efficient platform for the micropropagation of oil palm on a large scale. In addition, various genetic transformation techniques, including microprojectile bombardment, Agrobacterium tumefaciens mediated, Polyethylene glycol mediated mediated, and DNA microinjection, have been developed by optimizing various parameters for the efficient genetic transformation of oil palm. This review mainly emphasizes the methods established for in vitro propagation and genetic transformation of oil palm. Finally, we propose the application of the genome editing tool CRISPR/Cas9 to improve the various traits in this oil yielding crop.

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A review on factors affecting the Agrobacterium-mediated genetic transformation in ornamental monocotyledonous geophytes

Cited by 32 publications

References 77 publications

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

High frequency plant regeneration from leaf culture of <i>Neolamarckia cadamba</i>

Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

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