A review on isoprene in human breath

Mochalski, Paweł; King, Julian; Mayhew, Chris A.; Unterkofler, Karl

doi:10.1088/1752-7163/acc964

Cited by 30 publications

(11 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As presented in Figure b, the isoprene content in exhaled breath is less than 50 ppb in the calm state before exercise. After two minutes of exercise, the content of isoprene rises sharply and then gradually decreases as the exercise time increases, which is related to the metabolic process of isoprene in the human body and that further decreases close to the level before exercise. , These results illustrate that the developed mixed potential sensor holds application prospects in dynamic and real-time monitoring of the physiological metabolic status.…”

Section: Resultsmentioning

confidence: 69%

“…King et al proposed a physiological model of dynamic changes in isoprene concentration and predicted and studied the extrahepatic sources of isoprene. 6,7 Miekisch et al recently revealed that the source of isoprene in humans is attributable to muscle metabolic activity rather than hepatic cholesterol biosynthesis, which laid a theoretical foundation for the application of isoprene in biomarkers. 8−10 Therefore, due to the association of isoprene with muscle metabolism, noninvasive detection of isoprene concentrations in exhaled breath can help to optimize the training of professional athletes as well as guide therapeutic sport interventions for amateur or recreational sports enthusiasts, and is further expected to play a role in unraveling the interactions between exercise, musculoskeletal, and organ metabolism.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mixed Potential Type Isoprene Sensor for the Application in Real-Time Monitoring of Biomarker Gases

Jiang,

Li,

et al. 2024

ACS Sens.

View full text Add to dashboard Cite

Monitoring of isoprene in exhaled breath is expected to provide a noninvasive and painless method for dynamic monitoring of physiological and metabolic states during exercise. However, for real-time and portable detection of isoprene, gas sensors have become the best choice for gas detection technology, which are crucial to achieving the goal of anytime, anywhere, human-centered healthcare in the future. Here, we first report a mixed potential type isoprene sensor based on a Gd2Zr2O7 solid electrolyte and a CdSb2O6 sensing electrode, which enables sensitive detection for isoprene with sensitivities of −21.2 mV/ppm and −65.8 mV/decade in the range of 0.05−1 and 1−100 ppm. The sensing behavior of the sensor follows the mixed potential sensing mechanism and was further verified by the electrochemical polarization curves. The significant differentiation between the sensor response to exhaled breath of healthy individuals and simulated breath containing different concentrations of isoprene demonstrates the potential of the sensor for the detection of isoprene in exhaled breath. Simultaneously, monitoring of isoprene during exercise signifies the feasibility of the sensor in dynamic monitoring of physiological indicators, which is not only of great significance for optimizing training and guiding therapeutic exercise intervention in sporting scenarios but also expected to help further reveal the interaction between exercise, muscle, and organ metabolism in medicine. KEYWORDS: isoprene sensor, biomarker, Gd 2 Zr 2 O 7 , CdSb 2 O 6 , mixed potential

show abstract

Section: Resultsmentioning

confidence: 69%

mentioning

confidence: 99%

Mixed Potential Type Isoprene Sensor for the Application in Real-Time Monitoring of Biomarker Gases

Jiang,

Li,

et al. 2024

ACS Sens.

View full text Add to dashboard Cite

show abstract

“…Isoprene is known as a by-product of cholesterol biosynthesis [13]. It is mainly produced in the muscle tissue [51]. It was speculated that isoprene can be biomarker for diabetes, along with the acetone, but it was not confirmed yet, as isoprene biochemical pathway is not fully clear [51].…”

Section: Analytes Selectionmentioning

confidence: 99%

“…It is mainly produced in the muscle tissue [51]. It was speculated that isoprene can be biomarker for diabetes, along with the acetone, but it was not confirmed yet, as isoprene biochemical pathway is not fully clear [51]. However, there is potential to monitor breath isoprene in some CVD treatments that require lowering the cholesterol level [42].…”

Section: Analytes Selectionmentioning

confidence: 99%

Food and lifestyle impact on breath VOCs using portable mass spectrometer—pilot study across European countries

Aleksić,

Simeon,

Vujić

et al. 2023

J. Breath Res.

View full text Add to dashboard Cite

In the modern world, many people are changing old dietary and lifestyle habits to improve the quality of their living – to treat or just prevent possible diseases. The main goal of this pilot study was to assess the food and lifestyle impact on exhaled breath VOCs in various population groups. It was done by employing a recently validated portable membrane-inlet mass spectrometer – MIMS. Thus, the obtained results would also represent the additional confirmation for the employment of the new instrument in the breath analysis. The pilot study involved 151 participants across Europe, including people with overweight, obesity, type 2 diabetes mellitus, cardiovascular disease, people with poor-quality diet and professional athletes. Exhaled breath acetone, ethanol, isoprene, and n-pentane levels were determined in samples before the meal, and 120 min after the meal. Obtained basal ppbv values were mainly in accordance with previously reported, which confirms that MIMS instrument can be used in the breath analysis. Also, for people who have poor-quality diet and professional athletes, these were the first reported findings for the listed breath VOCs. Combining the quantified levels along with the information about the participants' lifestyle habits collected via questionnaire, an assessment of the food and lifestyle impact was obtained. Notable alteration in examined VOC levels upon meal consumption was detected in more than 70 % of all participants, with exception for isoprene, which was affected in about half of participants. Lifestyle parameters impact was examined using ANOVA on ranks test. Statistically significant differences in basal breath VOC levels were observed among all examined population groups. Also, n-pentane and ethanol levels significantly differed in people of different ages, as well as acetone levels in people with different physical activity habits. These findings are promising for further, more focused research using MIMS technique in breath analysis.

show abstract

“…The C 5 isoprene unit is the basic building block for terpenoid including steroid hormones biosynthesis that has been biologically essential for ubiquitous terrestrial life forms since billions of years in the course of evolution 9 . It is the second most abundant and endogenous volatile organic compound (VOC) in our exhaled breath 10 . Exhaled concentrations range between 100-300 ppbV in healthy adults.…”

Section: Introductionmentioning

confidence: 99%

Origin of breath isoprene in humans is revealed via multi-omic investigations

Sukul

Richter

Junghanß

et al. 2023

Preprint

View full text Add to dashboard Cite

Isoprene is amongst the most abundant volatile metabolites produced by plants and animals. Human exhaled isoprene is related to various pathophysiology but the origin was yet uncertain. Among 2000 individuals screened within consecutive mass-spectrometric studies, five healthy adults without breath isoprene were identified. Whole exome sequencing in these individuals revealed a shared homozygous (< 1% prevalent) IDI2 stop-gain mutation preventing conversion of isopentenyl diphosphate to dimethylallyl diphosphate (DMAPP) in cholesterol metabolism. Targeted sequencing depicted that the IDI2 variant is heterozygous in isoprene deficient blood-relatives and absent in unrelated isoprene normal adults. Wildtype IDI1 and cholesterol metabolism related serological parameters were normal in everyone. IDI2 determines isoprene production as DMAPP is the only source of isoprene and unlike plants, humans lack isoprene synthase and its homologue. Human IDI2 is only expressed in skeletal-myocyte peroxisomes and instant spikes in isoprene exhalation during muscle activity confirm the origin from muscular lipolytic cholesterol metabolism. Well-defined endogenous origin translated isoprene as a clinically interpretable breath biomarker.

show abstract

A review on isoprene in human breath

Abstract: We review the literature on isoprene in exhaled breath and discuss the current evidence and models that describe its endogenous origin and consequence for understanding isoprene levels and their variations in exhaled breath.

Cited by 30 publications

References 131 publications

Mixed Potential Type Isoprene Sensor for the Application in Real-Time Monitoring of Biomarker Gases

Mixed Potential Type Isoprene Sensor for the Application in Real-Time Monitoring of Biomarker Gases

Food and lifestyle impact on breath VOCs using portable mass spectrometer—pilot study across European countries

Origin of breath isoprene in humans is revealed via multi-omic investigations

Contact Info

Product

Resources

About