A Review on Magnetophoretic Immunoseparation

Wang, Yuhe; Chen, Qi; Gan, Chengqi; Yan, Bo; Han, Yaquan; Lin, Jianhan

doi:10.1166/jnn.2016.10930

Cited by 17 publications

(4 citation statements)

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“…The growing demand for immunoseparation methods and their market implementation is mainly due to the growing demand on the part of medicine and industrial biotechnology. Molecular tests are used in the analysis of cancer markers, diagnosis of bacterial diseases such as borreliosis and viral diseases such as hepatitis HCV, HBV or analysis of potential sources of contamination such as analysis of water samples [5].…”

Section: Legionella -Testing Methodology By Immunoseparation and Flow Cytometrymentioning

confidence: 99%

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Dabrowiecki¹,

Dąbrowiecka²,

Olszański³

et al. 2019

Polish Hyperbaric Research

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In the event of an epidemic of Legionnaires’ disease, prompt and unambiguous identification of the source of infection and immediate undertaking of repair actions is a necessary condition to limit and minimise the effects of the developing epidemic. In the classical method for determining the level of Legionella bacteria in water samples, the effectiveness of the reparative action (increase of the water temperature in the water supply system to 600C, additional chlorination) can only be confirmed after 14 days!!! Only by using the IMMS&FCM method can Legionella’s determination time be reduced to 2-4 hours, which is the most important factor in limiting the development of an epidemic.

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Section: Legionella -Testing Methodology By Immunoseparation and Flow Cytometrymentioning

confidence: 99%

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Dabrowiecki¹,

Dąbrowiecka²,

Olszański³

et al. 2019

Polish Hyperbaric Research

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“…During the magnetophoretic separation, there are gravity force, drag force, magnetic force, buoyancy force and inertia force acted on the MNPs [22] . The magnetic force (F m ) and the drag force (F d ) are much larger than the other forces, and they are considered to balance immediately [23] , i.e.…”

Section: Simulation Of the Magnetophoretic Separationmentioning

confidence: 99%

Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing

Wang

Cai

Wang

et al. 2019

International Journal of Agricultural and Biological Engineering

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In recent years, the outbreaks of foodborne diseases caused by pathogenic bacteria have made considerable economic losses and shown a threat to public health. The key to prevent and control these diseases is fast screening of pathogenic bacteria, which is usually performed with three procedures: sample collection, bacteria separation and bacteria detection. For sample collection, the national standard methods are often employed. For bacteria detection, currently available methods such as Polymerase Chain Reaction and Enzyme Linked Immuno-Sorbent Assay are often used. For bacteria separation, traditional methods such as filtration and centrifugation are not capable to specifically separate the target bacteria. However, food samples are very complicated and require efficient pretreatment for bacteria separation and concentration to achieve accurate and reliable results. The conventional immune magnetic separation method can be used to specifically separate the bacteria, but it still cannot meet the requirements for food sample pretreatment due to very low concentration of target bacteria in food. Therefore, this study developed an automatic and efficient immuno-separator of foodborne bacteria based on magnetophoresis and magnetic mixing, and E. coli O157:H7 was used as research model. A magnetic mixer was applied to facilitate the immunoreaction between the immune magnetic nanoparticles and the target bacteria cells, and a magnetophoretic separation tubing was utilized for magnetophoretic separation of the magnetic bacteria. Under the optimal mixing time of 20 min and the optimal flow rate of 50 µL/min, the separation efficiency of E. coli O157:H7 could be more than 90%, showing that the developed immuno-separator is promising to be applied for efficient separation of foodborne bacteria and can be easily extended for separation of other biological targets by using their specific antibodies.

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“…Sink/float magnetic immunoassays are based on two very simple techniques: immunomagnetic separations and density‐based sink/float tests. Immunomagnetic separation is a widely used technique for isolation and purification of bioreagents [2] but it has also been used for detection purposes in microfluidic setups [3] and biosensors [4] . Sink/float tests are one of the oldest testing platforms; e.g., sink/float tests for checking eggs’ freshness or fragmentation processes date back to Medieval Times [5] and the copper sulfate gravimetric test for measuring blood hemoglobin was first introduced in the 1940s [6] .…”

Section: Introductionmentioning

confidence: 99%

Sink/Float Magnetic Immunoassays for In‐Field Bioassays

Chorti

Christodouleas

2021

Angewandte Chemie

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Analytical tests/devices that are used outside laboratory settings are required to have a very simple analytical protocol to get clearance by regulatory authorities. This study describes sink/float magnetic immunoassays, a new type of rapid, mix‐and‐observe, instrument‐free tests for the detection of biomarkers in untreated biological samples that are very simple and might meet the simple‐to‐use criterion of authorities to be used in the field. These tests can tell whether an analyte is above or below a predetermined level within 25–45 minutes based on the sinking or floating of a mm‐sized sphere on the surface of which an immunoassay that uses reporter antibodies conjugated to superparamagnetic nanoparticles is performed. This manuscript describes the theory and proof‐of‐concept applications of sink/float magnetic immunoassays for the detection of C‐Reactive Protein, anti‐Treponema pallidum antibodies and E. coli bacteria.

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A Review on Magnetophoretic Immunoseparation

Cited by 17 publications

References 0 publications

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Development of automatic and efficient immuno-separator of foodborne pathogenic bacteria using magnetophoresis and magnetic mixing

Sink/Float Magnetic Immunoassays for In‐Field Bioassays

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