A review on microalgal growth stress parameters for sustainable biofuel production

Polat, Ece; Altınbaş, Mahmut

doi:10.1007/s10098-023-02535-x

Cited by 3 publications

(1 citation statement)

References 65 publications

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“…This interest intensified following the discovery of starchless mutants in C. reinhardtii, which, unlike their wild type (WT) counterparts, accumulate significant amounts of lipids, primarily triacylglycerols (TAGs), instead of starch. In addition, it has been found that microalgae produce lots of lipids under various stress conditions, such as nutrient depletion or osmotic stress (Maltsev et al, 2023;Polat and Altinbas, 2023;Singh et al, 2023). Based on these results, there has been a growing interest in metabolic and cultivation engineering to enhance carbon flux towards lipids from CO 2 (Dasan et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase

Shin,

Koh,

Park

et al. 2024

Front. Bioeng. Biotechnol.

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Microalgae, valued for their sustainability and CO2 fixation capabilities, are emerging as promising sources of biofuels and high-value compounds. This study aimed to boost lipid production in C. reinhardtii by overexpressing chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme in the Calvin cycle and glycolysis, under the control of a nitrogen-inducible NIT1 promoter, to positively impact overall carbon metabolism. The standout transformant, PNG#7, exhibited significantly increased lipid production under nitrogen starvation, with biomass rising by 44% and 76% on days 4 and 16, respectively. Fatty acid methyl ester (FAME) content in PNG#7 surged by 2.4-fold and 2.1-fold, notably surpassing the wild type (WT) in lipid productivity by 3.4 and 3.7 times on days 4 and 16, respectively. Transcriptome analysis revealed a tenfold increase in transgenic GAPDH expression and significant upregulation of genes involved in fatty acid and triacylglycerol synthesis, especially the gene encoding acyl-carrier protein gene (ACP, Cre13. g577100. t1.2). In contrast, genes related to cellulose synthesis were downregulated. Single Nucleotide Polymorphism (SNP)/Indel analysis indicated substantial DNA modifications, which likely contributed to the observed extensive transcriptomic and phenotypic changes. These findings suggest that overexpressing chloroplast GAPDH, coupled with genetic modifications, effectively enhances lipid synthesis in C. reinhardtii. This study not only underscores the potential of chloroplast GAPDH overexpression in microalgal lipid synthesis but also highlights the expansive potential of metabolic engineering in microalgae for biofuel production.

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Section: Introductionmentioning

confidence: 99%

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase

Shin,

Koh,

Park

et al. 2024

Front. Bioeng. Biotechnol.

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Hydrothermal liquefaction of wet microalgal biomass for biofuels and platform chemicals: advances and future prospects

Deepika,

Mrinal,

Pohrmen

et al. 2024

Discov Appl Sci

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Recent advances in hydrothermal liquefaction (HTL) have established this biomass conversion technology as a potent tool for the effective valorization and energy densification of varied feedstocks, ranging from lignocelluloses to microalgae and organic wastes. Emphasizing its application across biomass types, this exploration delves into the evolving landscape of HTL. Microalgae, recognized as a promising feedstock, offer a rich source of biomolecules, including lipids, carbohydrates, and proteins, making them particularly attractive for biofuel production. The comprehensive review explores the biofuel products and platform chemicals obtained through HTL of microalgae, delving into biodiesel production, bio-oil composition, characteristics, and to produce high-valued by-products. Challenges and limitations, such as reactor design, scalability issues, and the impact of microalgal composition on yields, are critically analyzed. The future prospects and research directions section envision advancements in HTL technology, integration with biorefinery processes, and the exploration of hybrid approaches for enhanced biofuel production. Overall, the paper emphasizes the promising potential of HTL for wet microalgal biomass and underscores the need for continued research to overcome existing challenges and unlock further opportunities in sustainable biofuel and platform chemical production.

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Enhanced lipid accumulation in microalgae Scenedesmus sp. under nitrogen limitation

Abrha,

Makaranga,

Jutur

2025

Enzyme and Microbial Technology

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A review on microalgal growth stress parameters for sustainable biofuel production

Cited by 3 publications

References 65 publications

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase

Hydrothermal liquefaction of wet microalgal biomass for biofuels and platform chemicals: advances and future prospects

Enhanced lipid accumulation in microalgae Scenedesmus sp. under nitrogen limitation

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