This book chapter aims to discuss the 3D ultrasound reconstruction and visualization. First, the various types of 3D ultrasound system are reviewed, such as mechanical, 2D array, position tracking-based freehand, and untrackedbased freehand. Second, the 3D ultrasound reconstruction technique or pipeline used by the current existing system, which includes the data acquisition, data preprocessing, reconstruction method and 3D visualization, is discussed. The reconstruction method and 3D visualization will be emphasized. The reconstruction method includes the pixel-based method, volume-based method, and function-based method, accompanied with their benefits and drawbacks. In the 3D visualization, methods such as multiplanar reformatting, volume rendering, and surface rendering are presented. Lastly, its application in the medical field is reviewed as well.