A review on recent developments for biomolecule separation at analytical scale using microfluidic devices

Tetala, Kishore K.R.; Vijayalakshmi, M.A.

doi:10.1016/j.aca.2015.11.037

Cited by 82 publications

(49 citation statements)

References 112 publications

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“…Recent examples include environmental analysis [1], biomolecular separations [2, 3], and mobile heath diagnostics [4]. …”

Section: Introductionmentioning

confidence: 99%

“…By replacing conventional analysis techniques with lab-on-a-chip technologies, it may be possible to achieve significant reductions in the size, cost, and complexity of automated QC testing platforms, and potentially to increase sensitivity [2, 23]. Commercial microscale devices already exist for testing of endotoxins [24], and there have been recent efforts to miniaturize some of the other tests, including radioactivity measurement [25], radioisotope identity (half-life) test [25], pH test [26], color and clarity test [26], and Kryptofix 2.2.2 test [27].…”

Section: Introductionmentioning

confidence: 99%

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Toward miniaturized analysis of chemical identity and purity of radiopharmaceuticals via microchip electrophoresis

Cheung

et al. 2018

Anal Bioanal Chem

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Miniaturized synthesis of positron emission tomography (PET) tracers is poised to offer numerous advantages including reduced tracer production costs and increased availability of diverse tracers. While many steps of the tracer production process have been miniaturized, there has been relatively little development of microscale systems for the quality control (QC) testing process that is required by regulatory agencies to ensure purity, identity, and biological safety of the radiotracer before use in human subjects. Every batch must be tested, and in contrast with ordinary pharmaceuticals, the whole set of tests of radiopharmaceuticals must be completed within a short-period of time to minimize losses due to radioactive decay. By replacing conventional techniques with microscale analytical ones, it may be possible to significantly reduce instrument cost, conserve lab space, shorten analysis times, and streamline this aspect of PET tracer production. We focus in this work on miniaturizing the subset of QC tests for chemical identity and purity. These tests generally require high-resolution chromatographic separation prior to detection to enable the approach to be applied to many different tracers (and their impurities), and have not yet, to the best of our knowledge, been tackled in microfluidic systems. Toward this end, we previously explored the feasibility of using the technique of capillary electrophoresis (CE) as a replacement for the "gold standard" approach of using high-performance liquid chromatography (HPLC) since CE offers similar separating power, flexibility, and sensitivity, but can readily be implemented in a microchip format. Using a conventional CE system, we previously demonstrated the successful separation of non-radioactive version of a clinical PET tracer, 3'-deoxy-3'-fluorothymidine (FLT), from its known by-products, and the separation of the PET tracer 1-(2'-deoxy-2'-fluoro-β-D-arabinofuranosyl)-cytosine (D-FAC) from its α-isomer, with sensitivity nearly as good as HPLC. Building on this feasibility study, in this paper, we describe the first effort to miniaturize the chemical identity and purity tests by using microchip electrophoresis (MCE). The fully automated proof-of-concept system comprises a chip for sample injection, a separation capillary, and an optical detection chip. Using the same model compound (FLT and its known by-products), we demonstrate that samples can be injected, separated, and detected, and show the potential to match the performance of HPLC. Addition of a radiation detector in the future would enable analysis of radiochemical identity and purity in the same device. We envision that eventually this MCE method could be combined with other miniaturized QC tests into a compact integrated system for automated routine QC testing of radiopharmaceuticals in the future. Graphical abstract Miniaturized quality control (QC) testing of batches of radiopharmaceuticals via microfluidic analysis. The proof-of-concept hybrid microchip electrophoresis (MCE) device demonstrated the fea...

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“…Recent examples include environmental analysis [1], biomolecular separations [2, 3], and mobile heath diagnostics [4]. …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toward miniaturized analysis of chemical identity and purity of radiopharmaceuticals via microchip electrophoresis

Cheung

et al. 2018

Anal Bioanal Chem

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“…Various separation techniques have been developed and explored in microfluidic systems for analysis of biomarkers [3, 4]. Molecular separation techniques in microfluidics discussed in this section are summarized in Table III.…”

Section: Molecular Separation Techniquesmentioning

confidence: 99%

“…Biomarker analysis has been one of the most actively pursued applications in miniaturization of chemical analyzers. A number of microfluidic systems have been reported recently that can perform sample preparation steps like purification, preconcentration and labeling on a chip prior to quantitation [1–3]. Separation techniques have also advanced for the analysis of molecular biomarkers in a microfluidic setup.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

142

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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“…7 Articles involving only channel wall coatings, packed columns or pillar structures are not included here, as other recent reviews cover these areas. [20][21][22] Advances in monolith material development, and use for SPE, separations, and reactors are reviewed, as are selected papers on the use of soft porous materials in analyzing for subcellular and larger biostructures.…”

Section: Introductionmentioning

confidence: 99%

Advances in monoliths and related porous materials for microfluidics

2016

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In recent years, the use of monolithic porous polymers has seen significant growth. These materials present a highly useful support for various analytical and biochemical applications. Since their introduction, various approaches have been introduced to produce monoliths in a broad range of materials. Simple preparation has enabled their easy implementation in microchannels, extending the range of applications where microfluidics can be successfully utilized. This review summarizes progress regarding monoliths and related porous materials in the field of microfluidics between 2010 and 2015. Recent developments in monolith preparation, solid-phase extraction, separations, and catalysis are critically discussed. Finally, a brief overview of the use of these porous materials for analysis of subcellular and larger structures is given.

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A review on recent developments for biomolecule separation at analytical scale using microfluidic devices

Cited by 82 publications

References 112 publications

Toward miniaturized analysis of chemical identity and purity of radiopharmaceuticals via microchip electrophoresis

Toward miniaturized analysis of chemical identity and purity of radiopharmaceuticals via microchip electrophoresis

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Advances in monoliths and related porous materials for microfluidics

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